染色于

Brains were removed cut on a freezing microtome at a thickness of 30 m. Immunofluorescence technique was used to detect BrdU and Nestin, NSE and GFAP were detected by immunohistochemistry.

取10一巧代的细胞，BrdU标记后在立体定向下通过细针移植入成年鼠的纹状体和大脑皮层，分别于移植后1周和6周取脑行冰冻切片，行BrdU和Nestin免疫荧光检测，以及NSE和GFAP免疫组化染色。

Among the 20 samples of IGF- 1 group, a 8-cell stage embryo, a morula and two blastula were found as positive by the red colour appearance .

IGF上的表达 2～8细胞胚胎 18个，桑堪胚 1个，囊胚 2个，共计 ZI个胚胎用于＊F2 检测，阳性表达14个，其中强阳性表达7个，弱阳性表达7个，分布于各阶段胚胎，在胚胎细胞中呈现深、浅程度不同的橘红色染色。l级和 2级胚胎 IGFZmRNA的阳性表达差异有显著性。

While the day the mice in non-pregnant group sacrificed,the mice in pregnant group were put in a cage to cohabit with male mice.The pregnant group were executed after conception 6 days to observe nidation condition of pregnant mice,and the uterus were taken to make paraffin sections,HE staining,and the sectiond were observed under ...更多 light microscope and image was analyzed.

在非受孕组雌鼠处死取材的当天，受孕组雌鼠与雄鼠合笼，于受孕后6 d处死受孕组小鼠，观察孕鼠着床情况，取子宫做成石蜡切片，HE染色，光镜观察并进行图像分析。

They are often morphologically distinct from A chromosomes, being smallerand more highly heterochromatic in most cases. B chromosomes do not pair with Achromosomes, and they are inherited in a non-Mendelian way, exhibiting meiotic andmitotic instability and nondisjunction. However, B chromosome DNA is quiteidentical to the corresponding sequence on the A chromosome complement. So far afew B chromosome specific DNA sequence have been identified. Specific DNAsequences on B chromosome have been the attractive research area on Bchromosomes.

现已在千余种植物和近三百种动物中被发现。B染色体与物种中正常染色体不同：独立于整倍体基因组之外，减数分裂时不与A染色体发生联会和配对，细胞分裂后期不分离，非孟德尔遗传，富含异染色质，不含对宿主主要性状有影响的基因等。B染色体DNA的分子组成既与A染色体极为相似，具有A染色体DNA分子组成的一般特征：富含重复序列和转座成分，染色体三大功能组件DNA高度同源；又与之相区别，含有B染色体特异的DNA序列，这些DNA序列可以为探讨B染色体的起源和进化提供有价值的信息。

Results:Typical apoptotic cellular changes were found after As2O3 treatment,transmission electron microscopy showed nucleole disappeared,chromatin condensated under nucleonic membrane.

结果：经As2O3作用后VSMCs出现典型的凋亡变化，透射电镜下可见细胞核仁消失，染色质浓缩聚集于核膜下。

Daxx can be localized in promyleocytic leukaemia protein oncogenic domains, nucleoplasm. nucleolus, cytoplasm, and heterochromatin.

Daxx可定位于早幼粒细胞白血病蛋白核体、核质、核仁、胞浆和异染色质。

Conversely, the immunofluorescence of FUS, a DNA and RNA binding protein known to be localised exclusively in the nucleoplasm,[18] didnot co-localised with [Pt(L3)Cl]+ staining (Figure 3G and H).

相反，FUS（一种已知排他定位于核原生质中的DNA和RNA结合的蛋白质[18]）的免疫荧光与[Pt(L3)Cl]+染色不共定位(图3G和H)。

The spiny eel , belonging to Perciformes, Osteichthyes, is a kind of little freshwater fish distributed in Southern China. In 1998, Our lab detected XX/XY differentiated sex chromosomes in this specie with karyotypic analysis, and then with chromosomal binding and SC observation, we identified that its two largest metacentric chromosomes were X, Y sex chromosomes in 1999. X, Y chromosomes differ each other not only in the distribution of heterochromatin but also in their shapes, which make them could be recognized directly under the light-microscope without any banding treatment.

刺鳅，属鲈形目、刺鳅科(Perciformes，Mastacembelidae)，是广泛分布于中国南方的小型淡水鱼类。1998年，我室通过核型分析，首次发现该物种具有明显分化的异形性染色体，其性别决定类型为XX／XY型。1999年，本室通过染色体显带、精母细胞联会复合体观察，再次确认刺鳅染色体组中最大的一对中部着丝粒染色体为X、Y性染色体，两者不仅已经出现结构异染色质分布上的不同，同时在外部形态上也存在明显差异，识别特征鲜明，在光镜观察下无需显带即能清晰辨认性染色体。

MAIN OUTCOME MEASURES: The seeded cells were observed under inverted microscope; at 1, 2, 3 weeks after seeding, the BMSCs were treated with 4% paraformaldehyde and stained with hematoxylin-eosin; The protein content in seeded cells was determined by bicinchoninic acid assay, and the content of DNA was quantified using Hoechst33258 assay at 5, 10, 14 days after culture.

主要观察指标：倒置显微镜下观察细胞形态；于培养1，2，3周采用40 g/L多聚甲醛固定，常规组织切片，苏木精-伊红染色；在培养5，10，14 d用Hoechs33258荧光法定量测定细胞内DNA含量，BCA法测定蛋白质含量。

Results Amyloid polyneuropathy is chronic progressive peripheral neuropathy,can be accompanied by injured of cardiovascular system, gastrointestinal tract and kidneys;pathological changes showed:Kongorot staining positive,amyloid deposited in endoneurium,perineurium and vascular adventitia,a marked reduction in the number of myelinated fibers and unmyelinated fibers,segmental demylinated.

结果 本病为慢性进行性周围神经病，可伴有心、胃肠、肾等损伤。病理改变示刚果红染色阳性，可见类淀粉物沉积于神经内膜、束膜及血管壁外膜，有髓及无髓纤维减少，节段性脱髓鞘。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐