染色于

The equipment is applicable for a variety of fabrics in pre-and post-treatment and dyeing process at a constant temperature.

本机适用于各种织物在常温条件下的前后处理及进行染色等工艺。

RESULTS: The AF cells cultured in different substrates were morphologically undistinguishable. Toluidine blue staining showed that there was also no difference between AF cells cultured on flexible silicone membranes and in plastic plates. The cells growing in different substrates expressed the same levels of collagen typeⅠ,Ⅱ and aggrecan mRNA and integrin β1. AF cells grew well on silicone membranes.

结果：种植于不同基质的纤维环细胞形态学观察无明显差别；在两种基质上的纤维环细胞甲苯胺蓝染色无差异；Ⅰ，Ⅱ型胶原及聚集蛋白聚糖mRNA及细胞膜整合素β1的表达亦无明显差异；纤维环细胞可以黏附于硅橡胶膜上生长。

Methods The skeletal muscle stem cells were enzymatically isolated with collagen and dispase, and then purified by different adhesion time from 6 female SD rats. The purified cells were transinfected with adenovirus carrying lac-Z gene, and injected into the bladder neck of 6 autologous and 4 allogeneic rats. Tissues of blader neck and ureter were obtained in sacrified rats after 5 and 15 days, histological examination and X-gal staining were performed.

采用混合酶消化及反复差速贴壁法分离、纯化培养SD大鼠骨骼肌干细胞，然后用携带lac-Z基因的腺病毒载体转染肌干细胞，待转染成功后将携带lac-Z基因的骨骼肌干细胞注射于自体和同种异体的膀胱颈及后尿道周围，分别于注射后5、15d，处死大鼠取出膀胱颈及后尿道，行组织学检查及X-gal染色。

Removed veins research demonstrate the main reason of transplanted vessel stenosis is the hyperplasic of endothelium. Which is a popular reaction when set vein into arteral circulation. It is caused by the transmission of smooth muscle cell to endothelium and proliferate and release extracellular matrix. These are two characteristics in pathology erg middle layer of sue proliferates and migrate to endothelium in early and excel of end thulium aggregate in cater stage. The colic and the motivation of end thulium hyper is the hyper of sac.

在mRNA水平，随着移植术后时间的推移，ECE的表达逐渐增高，手术后1W-2W达到高峰2W后表达逐渐下降，于8W左右趋于平衡，ET-1的表达与ECE的动态变化过程相似，亦于术手1W-2W达到高峰，在8W左右趋于平稳在蛋白质水平，以ET-1抗体免疫组织化学染色结果显示ET-1主要在血管内收细胞及平滑细胞胞浆中表达，尤其是中膜平滑肌细胞更为明显，呈棕黄色颗粒。

The results showed that in the control and experimental groups, HMGB1 immunoreactivity was all diffusely distributed in the cytoplasm of neuroepithelial cells and mesenchymal cells surrounded the neural tube at 8,16 and 24 hours after water bath treatment.

将孕鼠随机分为实验组和对照组，分别于水浴处理后8、16、24、40、64 h（相当于胚龄第8、8.5、9、9.5、10.5 d）处死孕鼠，剖腹取鼠胚，制备石蜡切片，应用免疫荧光染色技术检测NTDs发生过程中HMGB1在神经上皮细胞中的表达变化。

Results:①The amount of human colon carcinoma cell line SW480 treated by quercetin decreased. The morphology of partial SW480 cells was shrunk volume, integrated cell membrane, condensed cytoplasm, pyknotic chromatin, nuclear fragmentation. Apoptotic Corpuscles were found by electron microscope.②MTT colorimetric assay showed quercetin inhibited the growth of human colon carcinoma cell line SW480 in a time- and dose-dependent manner when the concentration of quercetin was 30、60、90μmol/L.③Flow cytometry analysis showed the cell cycle of SW480 cell was restricted in G1/S. G0/G1 phase rate increased and S phase rate decreased with increasing concentration of quercetin and time lasting.④ Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased. With increasing concentration of quercetin, the secretion of MMP-2 and MMP-9 decreased.⑤Immunohistochemistry method demonstrated the position expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time- and dose-dependent manner.

研究结果：经槲皮素处理的人结肠癌SW480细胞数量减少，部分细胞体积缩小，细胞膜完整，胞浆浓缩，核染色质固缩，细胞核碎裂，形成凋亡小体；MTT法检测显示当作用浓度为30μmol/L~90μmol/L时，槲皮素对人结肠癌SW480细胞的生长有抑制作用，其抑制作用随着作用浓度的增加和作用时间的延长而增强；流式细胞学发现槲皮素主要作用于人结肠癌SW480细胞周期的G1/S期，大部分细胞被阻断于S期，随药物浓度的升高和作用时间的延长，G0/G1期细胞比例逐渐增加，S期细胞比例逐渐减少；酶谱分析法检测显示不同浓度的槲皮素能够抑制人结肠癌SW480细胞分泌MMP-2及MMP-9，随浓度的升高，MMP-2及MMP-9的分泌量减少；免疫组织化学法显示不同浓度的槲皮素处理人结肠癌SW480细胞后，Cathepsin-D的表达随药物浓度的升高和作用时间的延长而降低。

Objective To investigate the difference in the distribution, amount and morphology of immunocytes in fetal skin, normal adult skin and hypertrophic scar, and to probe into their roles in fetal scarless wound healing from the dermatological and immunological point of view.

发育各期胎儿皮肤中均未见CD3；成人皮肤中可见少量CD3[(24±8)个/400倍视野]，主要分布于表皮基底层；HS组织中CD3较多[(69±25)个/400倍视野]，常聚集成片状，主要分布于真皮乳头层，在小血管周围呈袖套状分布，数量和染色强度大于成人皮肤(P.01)。

The Etest is an altenmative to agar dilution method for gonococci susceptibility testing to ceftriaxone,penicillin and ciprofloxacin due to its rapid,effectiveness and significant relationship with agar dilution method.

菌株来源实验菌株均从我所STD门诊患者的泌尿生殖道分泌物中分离得到，所有菌株经革兰染色、氧化酶试验和糖发酵试验证实为淋球菌传代一次，洗于脱脂牛奶中，于- 78℃低温冰箱冻存备用。2 。

The left legs were taken for the biomechanics test on the EnduraTEC ELF3200, the biggest vertical deformation and stiffness were calculated, the number of empty osteocyte lacunas was calculated, and the cumulative absorbance value was compared between two groups.

分别于2，4，6，8周处死大鼠，取右侧股骨头标本，于EnduraTEC ELF3200生物力学材料动态力学性能测试系统行压力测定，计算样本纵向最大位移、刚度吸收等生物力学参数，计算空骨陷窝数，并行免疫组织化学法Bcl-2染色，比较不同组之间的累计吸光度值。

Plant in 2000, the same year in April a formal trial production run success, has now developed into an internationally advanced level in Japan and some woolen three sets of Italian textile machinery equipment and advanced casual matching wool equipment, enterprise management personnel have 20 years old wool and transferred to a certain extent the production of management experience and skilled personnel, the company has continued to expand the scale of operation in June 2004 will be Lianyi Shandong Oriental Wool Company Limited acquisition, is now known as the; Linyi Lianxing Textiles Ltd., equipment for Japan in Beijing and 90 inches carding machine and three sets of two mule spinning machine and associated auxiliary equipment, while at 206 State Road, Linyi new levy of land 40 acres, purchased the domestic advanced level of the day and hair ray machine, high-speed twisting machine, the new joint-stock factory.

于2000年建厂，同年4月正式试产运行成功，现已发展到具有国际先进水平的日本粗纺三台套及部分意大利纺机设备和相配套的先进散毛染色设备，企业管理人员是有20多年的老毛纺调入且具有一定的管理经验和生产技术的人员，公司近年继续扩大了经营规模，于2004年6月将山东东方联益毛纺有限公司收购，现已更名为；临沂联兴纺织有限公司，设备为日本京和90英寸梳毛机两台和三台套的走锭纺纱机及配套辅助设备，同时在临沂206国道新征土地40亩，购进国内先进水平的日发并线机、高速倍捻机，新建合股分厂。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。