染色于

Milk fiber.The influence of pH value,dyeing temperature and neutral electrolytes on percent exhaustion were studied.The dyeing rates and buildingup properties of these dyes were also discussed.The effect of pH value was prominent on percent exhaustion,showing that the electrovalent bond between the dyes and the casein component of the fiber played an important role in the uptake of dyes.The ...

染液pH值、染色温度、元明粉等对上染率的影响，以及这两类染料在牛奶丝上的染色速度和提升性能的研究表明，牛奶丝染色受pH值影响很大，说明染料与酪素蛋白之间的离子键结合对染料上染起重要作用；元明粉在各pH值下对上染率的影响显示，牛奶丝的等电点约为4；酸性染料对牛奶丝的初染率高、初染速度快，但80℃恒温下的上染速度却慢于蚕丝和羊绒；牛奶丝染色对pH值的敏感性大于蚕丝和羊绒，且其酸性染料上的提升力较蚕丝和羊绒差。

In the experimental tectum, the expression of GFAP in the right SO was higher than the expression of GFAP in the left SO during 1d-60d after after optic nerve damage. The expression of GFAP in the right SO was familiar with the expression of GFAP in the left SO during 85d after optic nerve damage, but the expression of GFAP in SO in the experimental tectum was higher than that in the normal tectum. The result of GFAP expression in the experimental retina and tectum showed that AStrocyte took part in the optic nerve regeneration and it might play an important role in optic nerve regeneration accidents.

在损伤1d后视网膜的神经纤维层和外核层外缘可见较为深的GFAP染色，在损伤3d后视网膜从内网层经内核层至外网层出现稀疏的垂直于视网膜长轴分布的线条状GFAP染色，随时间推移，伤后5d、7d、14d、28d上述染色进一步加深密集，到伤后60d和85d上述染色减弱，阴性对照的视网膜各层未见GFAP阳性染色；(5)正常鲫鱼视顶盖中在SO层内有较浅的黄棕色线条状GFAP染色，在SFGC层

NGFR positive nerve fibres were markedly reduced or absent in muscular layer and submucous layer while the hypertrophic nerve trunks in myenteric and submucosal plexuses showed a sheath of intense staining in the aganglionic colon from patients with HD.

结果 丰富的NGFR染色阳性神经纤维分布于正常结肠环肌层及粘膜下层，少量分布于纵肌层，NGFR染色阳性神经元分布于肌间神经丛及粘膜下神经丛；NGFR 染色阳性神经纤维在HD无肌间神经节细胞肠段肌层及粘膜下层内明显减少或缺如，而肌间肥大的神经干及粘膜下神经干为NGFR染色阳性，并且在其外周可见一强阳性染色带。

Staining extent and intensity were evaluated semiquantitatively and mean values for each parameter were calculated. Immunostaining with D2-40 showed positivity in 100% of skeletal myxoid chondrosarcomas, 96% of enchondromas, 95% of low-grade chondrosarcomas, 80% of chordoid meningiomas, and 75% of chordoid gliomas. Staining with S100 demonstrated diffuse, strong positivity in all (100%) chordoid gliomas, skeletal myxoid chondrosarcomas, low-grade chondrosarcomas, and enchondromas, 94% of chordomas, and 81% of extraskeletal myxoid chondrosarcomas, with focal, moderate staining in 40% of chordoid meningiomas.

我们半定量地评估了这些免疫染色的广度和强度，并且计算了它们各自的平均值。D2-40阳性表达于100%例骨的黏液样软骨肉瘤、96%例内生性软骨瘤、95%例低级别软骨肉瘤、80%例脊索样脑膜瘤和75%例脊索样胶质瘤。S100染色弥漫且强烈地表达于所有的（100%）脊索样胶质瘤、骨的黏液样软骨肉瘤、低级别软骨肉瘤和内生性软骨瘤，94%例脊索瘤，81%例骨外黏液样软骨肉瘤，还有，局灶性、中度表达于40%例黏液样脑膜瘤。

All tumors were histologically high grade (6 grade III and 10 grade IV). Three tumors showed heterologous elements, 2 osseous, and 1 rhabdomyoblastic. More often scattered than diffuse, S-100 protein staining was noted in 11 of 16 tumors and variable collagen IV staining in 10 of the 16. Immunoreactivity for p53 protein was diffuse and strong in 7 of 11 tumors. Twelve patients died within 17 months to 3 years of diagnosis, 1 was lost to follow-up, 2 are very recent cases, and 2 patients are currently alive, 1 after 2 recurrences, and another with spinal leptomeningeal metastases.

所有病例在组织学上都是高级别（6例III级和10例IV级）。3例肿瘤含有其他成分：2例含骨和1例含 rhabdomyoblastic.S-100蛋白染色显色于16例肿瘤中的11例，IV型胶原染色显色于16例中的10例，这些染色通常分散而不弥散。11例肿瘤中7例的p53蛋白免疫反应弥散且强烈。12例病人死于确诊后的17个月至3年，1例随访失败，2例是最近的病例，还有2例现在还活着，其中1例复发，另1例发生脊髓脑膜转移。

The general situations of the eye were observed and the corneal thickness were measured with ultrasonic corneal pachymeter after the animal models was established. After a week, the corneas were removed after the experimental animals are put to death. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution , HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

术后观察眼球大体情况、测量角膜厚度。1周后处死实验动物取角膜，用茜素红-台盼蓝染色染色行角膜内皮细胞计数；用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；实时荧光定量PCR检测AQP-1mRNA在角膜内皮细胞表达的改变；并于正常对照组角膜比较。

Compared to the silk, moisture regains, level dyeing and thermal behavior of raw silk grafted by MA was better. The K/S value increased a little, the fastnesses decreased a little. But the level dyeing of raw silk grafted by EMA declined severely. Compared to the grafted degummed silk, the moisture regains and level dyeing improved a little.

MA生丝增重丝相对于熟丝吸湿性提高、酸性染料染色的K/S值略有上升、匀染性提高、各种染色牢度变化不明显、热稳定性有所提高；而EMA生丝增重丝吸湿性下降、酸性染料染色的K/S略有上升、匀染性变差、各种染色牢度略有下降、热稳定性有所提升。

Results There was a similar distributive pattern of Neul, PPCA and β-gal in the inner ear. Neul intense staining was observed in the cochlear spiral ganglion cells, spiral limbus, spiral ligament, vestibular ganglion cells, cristae, maculae hair cells, and weak staining in inner hair cells, outer hair cells, supplying cells of the organ of Corti and stria vascularis. The intense staining of PPCA and β-gal were observed in the spiral ganglion and vestibular ganglion cells, and weak staining in the spiral limbus, spiral ligament, stria vascularis and organ of Corti. The inner ear exhibited no staining when Neul, PPCA and β-gal were deficient, respectively.

Neul最强的染色主要在螺旋神经节细胞、螺旋韧带、螺旋缘、前庭神经节细胞及壶腹嵴、球囊和椭园囊感觉毛细胞，较弱的染色分布于血管纹和Corti器内、外毛细胞及支持细胞；PPCA和β-gal在螺旋神经节和前庭神经节细胞有较强的染色，血管纹、螺旋韧带、螺旋缘和Corti器内、外毛细胞及支持细胞呈较弱的染色反应；各自酶缺乏时内耳免疫染色消失。

Finally,when the ultrasonic wave was applied in the fixation procedure,the fastness to rubbing and soaping were improved.

结果表明，超声波施加于阳离子改性阶段，可以缩短改性时间15 m in左右；超声波施加于染色阶段，可以明显提高涂料上染百分率及染色速率；超声波施加于固色阶段，可以提高湿摩擦牢度半级左右。

The ultrasonic wave applied in the dyeing process with plants indigo dye can not only improve the rubbing and soaping fastness, but also shorten the dyeing time, increase uptake rate and lower dyeing temperature.

将超声波技术应用于植物靛蓝染料的染色工艺，不仅可使染色样品的耐摩擦色牢度和耐皂洗色牢度有所提高，而且可缩短染色时间，提高上染率，降低染色温度。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。