染色

RESULTS: After differentiation of human adherent myoblasts by neural induction medium, no cells with a neural cell morphology (ie., small, refractile cell body with dendritic cell extensions) were seen. All remaining myoblasts cultured with neural induction medium, myoblasts with proliferation medium and myotubes with differentiation medium containing 20 mL/L HS were positive for β Tubulin Ⅲ. C2C12 myoblasts were negative for β Tubulin Ⅲ. In contrast, all the above cells were negative for the markers Neurofilament Mr 68×103 and GFAP.

结果：用诱导分化液作用后，未见小的、伴有突起的放射状形态的神经细胞；抗β Tubulin Ⅲ对经神经元胶质细胞诱导分化液作用后的人成肌细胞、增殖培养液培养后的各代人成肌细胞及仅含20 mL/L HS分化液分化形成的肌管细胞染色均为阳性； C2C12细胞β Tubulin Ⅲ抗体染色阴性；上述所有细胞抗Neurofilament Mr 68×103和抗GFAP染色均为阴性。

The denervated extensor digitorum brevis and the extensor hallucis brevis of the rhesus monkey were autotransplanted in the sacrospinal muscle,and they were biopsied at intervals and stained with HE,Bielshowsky and enzyme.

将恒河猴的双足踇、趾短伸肌8块分2组去神经后游离移植于骶棘肌内，定期活检行光镜(HE染色、银染色、酶染色)及电镜观察肌纤维及神经纤维。

Immunohistochemical assay were performed with HGV NS5 McAb. Results One of four samples showed positive staining, some of which located in cytoplasm and some of which in cytonucleus and cytomembrane. The number of positive staining cells are not more. More of them disrtibuted scatteredly, some of them located.

结果 1例呈阳性染色，光镜观察显示：特异染色大部分定位于肝细胞胞浆中，部分有核着色和膜型染色，呈黄色或棕黄色颗粒状，阳性细胞数量少，大多散在分布，部分呈灶性分布；阳性细胞周围可见较多淋巴细胞浸润。

The characteristics of package dyeing of scutching tow are analyzed and possible defects associated with it as well as preventive measures are also given.

分析了油丝筒子纱染色的特点及在染色中可能产生的疵点和预防措施，并着重论述了油丝筒子纱染色的工艺及应用。

Describes real silk package dyeing principle and analyses the pump lift and delivery, controlsystem, tensionless winding and degumming/dyeing mechanism, with a systematic presentation ofthe development and application of the dyeing technology.

论述了真丝筒子染色的原理，并对染色机的水泵扬程及流量，控制系统，松式卷绕和脱胶染色机理进行了分析。

Results: Irradiated part of liver showed radiation-induced liver injury tested by H. E.,trichrome and TGF-β1 immunohistochemical staining.

结果：H.E。染色、TGF-β1免疫组化染色和三色染色检测出了照射区肝脏的肝放射性损伤。

Methods: Fresh stools of the patients suffered from diarrhea were examined with direct wet-mount, iodine staining and trichrome staining.

收集腹泻患者当日新鲜大便，用生理盐水直接涂片、碘液染色及三色酸染色法染色镜检。

In this study, we found that the positive immunostaining for αSMA was increased in group DN from week 4 onward, mainly located in dilated tubuli, which was more evident at week 8. The double staining for CTGF and p27kip1 showed that the increased positive staining for both antibodies was more evident in tubuli and some glomeruli.

正常大鼠肾脏可以有少量细胞P27kip1染色阳性，本研究发现，在DN模型组，第1周起肾小球和肾小管P27kip1染色阳性细胞即显著增加（P＜0.01），且随着病程进展，P27kip1染色阳性细胞比例显著增加。

Using CCA, karyotype abnormalities were found in 18 (37.5%) of 48 cases, among which complex karyotypes were found in 4 (8.3%) cases, a deletion of chromosome 5/a deletion of the long arm of chromosome 5 (-5/5q-) in 5 (10.4%) cases,-7/7q- in 5 (10.4%) cases, trisomy 8 in 8 (16.6%) cases, 20q- in 2 (4.6%) cases, unconsistent translocations in 3 (6.2%) cases.

细胞遗传学分析检出染色体异常18例(37.5%)，其中复杂异常4例(8.3%)、5号染色体缺失或5号染色体长臂部分缺失5例(10.4%)、7号染色体缺失或7号染色体长臂部分缺失5例(10.4%)、8号染色体增加8例（16.6%）、20号染色体长臂部分缺失2例（4.6%）、不一致的易位3例（6.2%）。

The findings of the newly identified goose paramyxovirus strain GPMV/HBexperimental infection were as follows: The challenge caused high morbidity and highmortality.The clinical signs included severe depression, decreased appetite or anorexia,white,yellow and green diarrhea, with neural symptoms;pathological changes showed thathaemorrhage and ulceration of intestine mucosa, necrosis of spleen and pancreas,andedema of liver and kidney, uratic deposition in kidney.Eosinophilic inclusion bodies werefound in the cytoplasm of pancreas and a large quantities of fiber protein were found inintestine. Lots of hemosidefin was found in some area of liver and kidney. A number ofT、B lymphocytes reduced sharply in spleen,lymph nodes in dead geese. Transmissionalelectron microscope showed that some cells had the morphological characteristics ofmitochondria swelling, morphological changes were condensation of nuclear chromatininto dense masses,followed by its marginating against the nuclear envelope, andespecially concentration of cytoplasm association with lots of lipid droplet.

人工感染雏鹅实验中，雏鹅临床表现为高发病率和高死亡率，食欲和饮水减少，拉白色、黄绿色稀粪，伴有神经症状；常规组织学观察，脾脏和胰腺肿大，有白色坏死斑点，肠道出血和坏死，肝脏肿大变性，肾脏肿大变性有尿酸盐沉着等；用特殊染色和组织化学方法首次对人工感染鹅副粘病毒的雏鹅的重要脏器进行研究，结果表明：胰腺中发现病毒包涵体，肠道中大量纤维素以及胶原纤维增生，肾脏、肺脏大量含铁血黄素沉着，免疫器官如脾脏和淋巴结中T、B淋巴细胞大量减少；电镜下多种细胞呈现线粒体水肿，多种实质细胞的染色质固缩，染色质边集，特别是细胞内容物如脂肪滴增多的病理变化特征。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。