染料木黄酮

Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上，开展了如下未见文献报道的创新性的研究工作：(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究，建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法，与以前的报道相比，灵敏度和分析时间均有很大改善；(2)使用荧光素钠作为背景荧光试剂，建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法，扩大了激光诱导荧光检测的应用范围，对难衍生化合物的分离分析提供了一种新思路和新途径；(3)以荧光素钠作为背景荧光试剂，建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法；(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物，为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。

The proposed method surpassed other chromatographic alternatives in terms of limit of detection at least 10~3 folds with good reproducebility. Chapter 4 A capillary zone electrophoresis with indirect LIF detection method is developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside).

第四章建立了一种毛细管区带电泳—间接激光诱导荧光检测法同时测定6种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的新方法，扩大了LIF检测的应用范围。

The interaction between three small flavoind drug molecules, which are apigenin, galangin and genistein, and BSA is investigated with fluorescence spectroscopy using the drug's quenching on the endogenous fluorescence of serum albumin.

采用荧光光谱法，利用药物对牛血清白蛋白自身内源荧光的猝灭，研究了芹菜素、高良姜素和染料木素这三种黄酮药物小分子与牛血清白蛋白分子间的相互作用，研究发现药物分子与牛血清白蛋白分子之间发生了有效的静态猝灭。。。

The structures were elucidated by spectral analysis and chemical evidence.Result:Six compounds were isolated from the plant and identified as alpinumisoflavone,wighteone,daidzein,genistein,vittadinoside and stigmasterol.

结果 ：从该植物中分离得到6个化合物，分别鉴定为高山金链花素，4′，5，7 三羟基-6-异黄酮，大豆素，染料木素，维太菊苷和豆甾醇。

Genistein promotes osteoid mineralization,reduces the loss of Ca, P and Mg in the bone and prevent the generation of osteoporosis in unsexed rats

染料木黄酮促进去势大鼠类骨质矿化，减少骨中钙、磷、镁丢失，预防骨质疏松的发生。

My company's main products: Rhynchophylline Gouteng silymarin (ethanol extract And no residual acetone) silymarin (water-soluble) silybin resveratrol daidzein emodin Suanzaoren extract of soy isoflavones curcumin, artesunate - Song Shupi extract, Hypericum perforatum extract, wild chrysanthemum extract, camptothecin, hawthorn extract, ursolic acid, corn required to extract, Maidong extract, garlic extract, ginsenosides, Chinese wolfberry extract - And asked Jing extract, green tea extract, the extract of small BAI Ju, Kushen extract, valerian extract, Salicylaldehyde glycosides, podophyllotoxin, Monascus Abulimiti of admission, puerarin extract, extract of astragalus, bitter melon extract Of, the thorn Tribulus terrestris extract, the extract of Ginkgo biloba leaves, Zhishi extract, Icariin, grape seed extract, Salvia extract yam extract, blueberry extract, the extract of Andrographolide, white Liu admission of Piti , Filed on Schisandra, Gynostemma extract, the extract of Angelica, Trifolium pratense extract, the extract of Rhodiola, soil Fritillaria saponins, Huang Chunju extract, and Cimicifuga extract, Hypericum perforatum Extract, Polygonum cuspidatum glycosides, genistein, reserpine, synephrine, sinomenine, Ciwujia glycosides, yam saponin, puerarin, peeling hormones, Kawa Ti of admission, lutein, lycopene, pumpkin seeds extract, licorice extract, nettle extract, the extract baicalin, Osthol, Asarum, saw Mr Brown extract, water Zaojiao extract, rutin extract, purple cabbage extract , Motherwort extract, Yanhusuo extract, hops extract, the extract of Magnolia officinalis, Cyclovirobuxine wood extract, Ban Langen extract, ginger extract, dandelion extract, senna extract, aloe extract , Dangshen extract, the extract of honeysuckle, quercetin, etc.

我公司主营产品：钩藤 Gouteng ·水飞蓟素·水飞蓟素·水飞蓟宾·白藜芦醇·大豆甙元·大黄素·大豆异黄酮·酸枣仁提取物·姜黄素，青蒿素松树皮提取物，连翘提取物，野菊花提取物，喜树碱，山楂提取物，熊果酸，玉米须提取物，麦冬提取物，大蒜提取物，人参总皂甙，枸杞提取物，问荆提取物，绿茶提取物，小白菊提取物，苦参提取物，缬草提取物，水杨甙，鬼臼毒素，红曲米提取物，葛根提取物，黄芪提取物，苦瓜提取物，刺蒺藜提取物，银杏叶提取物，枳实提取物，淫羊藿甙，葡萄籽提取物，丹参提取物，山药提取物，越橘提取物，穿心莲提取物，白柳皮提取物，五味子提起物，绞股蓝提取物，当归提取物，红车轴草提取物，红景天提取物，土贝母总皂甙，黄春菊提取物，黑升麻提取物，贯叶连翘提取物，虎杖甙，染料木素，利血平，辛弗林，青藤碱，刺五加甙，薯蓣皂素，葛根素，脱皮激素，卡瓦提取物，叶黄素，番茄红素，南瓜籽提取物，甘草提取物，荨麻提取物，黄芩提取物，蛇床子，细辛，锯叶棕提取物，水皂角提取物，芦丁提取物，紫甘蓝提取物，益母草提取物，延胡索提取物，啤酒花提取物，厚朴提取物，黄杨木提取物，板兰根提取物，生姜提取物，蒲公英提取物，番泻叶提取物，芦荟提取物，党参提取物，金银花提取物，槲皮素，等等

These changes were prevented by genistein (a protein tyrosine kinase inhibitor) and antioxidant N-acetyl-L-cysteine, but promoted by sodium orthovanadate (a protein phosphatase inhibitor), which were administered to the SD rats 20 min before ischemia.

进一步的研究表明，缺血前20 min腹腔注射给药，然后缺血30 min，发现蛋白酪氨酸激酶抑制剂染料木黄酮和抗氧化剂N-乙酰半胱氨酸能显著地抑制核内STAT3的磷酸化水平及DNA结合活性的增加(磷酸化水平从2.3和2.5倍分别降为1.2和1.4倍， DNA结合活性则从2.8和3.7倍分别降为1.1和1.5倍)，而蛋白酪氨酸磷酸酶抑制剂矾酸钠则能明显地促进他们的增高(磷酸化水平从2.0倍增到3.4倍， DNA结合活性从3.1倍增为5.1倍)。

Genistein can stimulate human osteoblastic cell proliferation and differentiation in a dose and timedependent manner.

染料木黄酮能够促进人成骨细胞的增殖和分化，其促进作用存在时效和量效关系。

Objective To observed the effects of 5 hydroxyl group of genistein on recombinant human protein kinase CK2 holoenzyme activity.

目的观察染料木黄酮C5位上的羟基对其抑制重组人蛋白激酶CK2全酶活性的影响。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。