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The homology of nucleotides and amino-acid residue with the sequence in GenBank was 95.88% and 99.07%, 293 cells transfected with the recombinated plasmid express relucent green fluorescence.

转染293细胞后24-72h,可观察到明亮的绿色荧光,RT—PCR和Western blot分别从mRNA和蛋白水平上检测到frc基因在真核细胞中的表达。

Retouch:. The process of coloring the new growth on tinted or bleached hair.

润饰: 对染过或漂过的头发上新长出的头发进行补色的过程。

Rare elements and REE are mainly enriched in gray-black trachyte and less in other rocks. In addition, schistosity, pyritization, silicification and carbonation in gray-black trachyte are contributed to enrich rare element and REE. It is suggested that alkaline rocks in Hongyang area have the characteristics of subvocanics, and alkaline rocks in Hongyang and Miaoya areas all came from upper mantle and mixed with some substances of crust. Intrusion of alkaline rocks is related to the occurrence, development and evolvement of the deep rupture zone in the areas.

稀土、稀有元素主要富集于灰黑色粗面岩中,而在其他岩石类型中相对较少,同时岩石的片理化、黄铁矿化、硅化和碳酸盐化对稀土、稀有元素有进一步的富集作用;提出了本区的碱性岩组合具有次火山岩相的特征,讨论了本区碱性岩的成因,认为其与湖北庙垭地区的碱性岩可能均来自上地幔,并不同程度地混染了大陆壳的物质成分,其形成时代与断裂构造带的发生、发展和演化基本同步。

Used in one bath scouring and bleaching of chemical fiber yarn and fabric.

用在化纤纱线及织物的炼染一浴法上。

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明:①在适宜的浓度体外培养条件下,NSCs能形成典型的神经干细胞克隆球,Vimentin免疫荧光染色阳性,单靠丝裂原刺激或NSCs自我调节和分化诱导,不会产生典型的终末分化的成熟神经细胞;②海马源性的NSCs维持干细胞特性的时间更长,生物学特性更优;③传至第2~3代的NSCs尚未分化时移植最佳;④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞;⑤各种子代克隆球和细胞存在广泛的突触联系;⑥NSCs与OECs联合培养时,不需丝裂原刺激即能形成克隆球,获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞;⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化,但并不出现终末分化;⑧本研究培养条件不利于成纤维细胞、O4生长。

Objective To investigate the role of oxidative stress on renal injury induced by fluoride and the expression of Bcl-2 on the oxidative stress of renal tubular cells exposed to sodium fluoride at different concentration.

摘要目的观察不同染氟条件下大鼠肾小管上皮细胞氧化应激能力变化及Bcl-2 在氧化应激时的表达。

It shows that the rate of dye - uptake was good and the fastness to soap and solarization were comparable with black direct dyestuff L - 3BG.

结果表明,染料的上染率较高,染料的耐皂洗牢度和耐日晒牢度与直接黑L-3BG相当。

Results (1) The objective compound was synthesized, and its structure was confirmed by mass spectrography, infrared spectrography and nuclear magnetic resonancespectroscopy.

4用重组真核表达载体pLXSN-hBD2通过新型阳离子脂质体对大鼠气道上皮细胞进行转染,用PCR、Western blot检测hBD-2基因的整合与表达。

Sell your soul for that, do, dyed rags pinned round a squaw

为了那些用别针别在婆娘腰身上的染了色的节片,出卖你的灵魂吧。

Sell your soul for that, do, dyed rags pinned round a squaw.

为了那些用别针别在婆娘腰身上的染了色的节片,出卖你的灵魂吧。务必这么做。

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And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47:5 法老对约瑟说,你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法,同时,采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。

Hey Big Raven, The individual lies dont matter anymore - its ALL a tissue of lies in support of...

嘿大乌鸦,个别谎言的事不要再-其所有的组织的谎言,在支持。