柏木脑

The fir essential oil was extracted from fir root by steam distillation method, from which cedrol was extracted, and then, cedrol methyl ether was synthesized based on cedrol.

以杉木根为原料通过水蒸气蒸馏法提取杉木精油，从杉木根精油中精制提取柏木脑，再以柏木脑为原料合成甲基柏木醚。

Two steps were included in this reaction, first, sodium alkoxide was synthesized by th reaction between sodamide and cedrol, then cedrol methyl ether was synthesized by methylation reaction between sodium alkoxide and dimethyl sulfate.

反应分两步，首先氨基钠与柏木脑反应生成醇钠，然后醇钠与硫酸二甲酯进行甲基化反应生成甲基柏木醚。

Based on groups of single factor experiments, the yield could reached to 88.8% with the conditions: sodamide: cedrol :dimethyl sulfate = 1.6:1:0.9, refluxed 2h in first step reaction, and reacted at room temperature 1h after instillation of dimethyl sulfate, followed refluxed 1h in the second steps.

通过多组单因素实验发现氨基钠：柏木脑：硫酸二甲酯的摩尔比为1.6：1：0.9，第一步反应条件控制在回流2h，第二步反应先室温（25℃）滴加硫酸二甲酯后反应1h，再回流反应1h，得率可达88.8%。

The relative contents of thujopsene and cedrol were higher in P.orientalis extracted by SFE and MSD methods.No monoterpenes was found in the compounds extracted by SFE and SDE,and the wood resin acids were the major compounds in the compounds extracted by SE.

改装简易蒸馏样品和超临界CO_2萃取物以及并时蒸馏萃取物中含量最高的组分是罗汉柏烯和柏木脑，但是超临界CO_2萃取物中未检测出单萜；索氏提取法样品中含量最高的组分为非挥发性的树脂酸类物质。

ASES is 59% identical to Artemisia cyclase cDNA clone cASC125, 50% identical to epi-cedrol synthase from A. annua, 48% identical to amorpha-4, 11-diene synthase from A. Annua, 39% identical to the 5-epi-aristolechene synthase from tabacco, 38% identical to vetispiradiene synthase from H. Muticus, 41% identical to the δ-cadinene synthase from cotton. The coding region of cDNA was cloned into procaryotic expression vector pET30a and overexpressed in E. coli BL21 (DE3). The cyclase proteins extracted from bacterial culture were found largely in the insoluble protein fraction. The tissue specific expression patterns were analyzed by RT/PCR.

克隆的倍半萜合酶氨基酸序列与烟草马兜铃烯合酶、莨菪岩兰螺旋二烯合酶、棉花杜松烯合酶的一致性分别为39％，38％和41％；与青蒿柏木脑合酶、紫穗槐二烯合酶和一个推测的倍半萜合酶克隆cASC125的一致性为50％，48％和59％。cDNA编码区序列被克隆进原核表达载体pET-30a，并在大肠杆菌BL21（DE3）中诱导表达，但过量表达的蛋白主要是以不溶性蛋白形式存在。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。