枯草杆菌

Using the cooperations between sporulation and competence-forming related genes both from other pleitropic regulatory genes of Bacillus, hpr, degQa, degU/S 32and degR and from the Bacillus subtilis Ki-2-132. The series high-expressive genetical engineering host strains were constructed.

利用芽孢杆菌多效调控基因hpr、deqQa、degR、degU／532等和枯草杆菌Ki－2－132自身的积生孢、感受态相关基因的协调作用，构建了系列的高表达遗传工程宿主。

RESULTS The average bacteriacolony forming unit per milliliter in dialysis fluid from the dialyzer inlet , outlet and reverse osmosis was 486 CFU/ ml ,490 CFU/ ml and 103 CFU/ ml , respectively. The common isolated bacteria were Bacillus subtilis , diphtheroid bacilliand some Gram2negative bacilli.

结果 透析器入口处透析液的平均菌落数为486 CFU/ ml ，透析器出口处透析液的平均菌落数为490 CFU/ ml ，反渗水的平均菌落数为103 CFU/ ml ，常见细菌为枯草杆菌、类白喉杆菌及某些革兰阴性杆菌。

Both 50% boiling water extract and 50% alcohol extract of Zingiber Coral- linum Hance do not inhibtte the five tested bactreial species:Staphy.aureus, Strept.hemolytieus,B.subtilis,E.coli and S.typhus.

本文报告对珊瑚姜抑制细菌和真菌作用的研究。结果表明珊瑚姜50%水煎剂和50%酒精浸液对金黄色葡萄球菌、乙型溶血性链球菌、枯草杆菌、大肠杆菌和伤寒杆菌都无抑制作用。

The optimum hydrolytic conditions of razor clam for neutral protease were : enzyme concentration 2.4%, hydrolysis temperature 50癈, substrate concentration 1:3, pH 7 and reaction time 6 hrs with the degree of hydrolysis and extraction rate of total nitrogen 42.46% and 83.04% respectively; and foracid protease they were: en/yme concentration 6.0%, hydrolysis temperature 50癈, substrate concentration 1:4, pH3.5 and reaction time 5 hrs with the degree of hydrolysis and extraction rate of total nitrogen 46.37% and 87.94% respectively, the hydrolysate being clear light yellow. For the combination of two kinds of proteases they were: first hydrolyzing with 2.6% f 1 avouryme for 3 hrs, then hydrolyzing with 2.4% neutral protease for 3 hrs with the degree of hydrolysis and extraction rtiie of total nitrogen 4-1.57% and 86.95% respectively.

最后综合考虑了水解率、总氮回收率和水解液的色泽、澄清度等指标，得出了较适宜的酶种为单酶以枯草杆菌中性蛋白酶和酸性蛋白酶效果较好，双酶以风味蛋白酶与枯草杆菌中性蛋白酶双酶组合水解效果较好，其适宜的水解工艺条件分别为：枯草杆菌中性蛋白酶加酶量为2.4%，水解温度50℃，料水比1：3，pH值中性条件下水解6h，绕蛙蛋白质的水解率和总氮回收率分别为42.46%和83.04%；酸性蛋白酶水解编蛙肉的适宜的工艺条件为加酶量6.0%、水解温度50℃，料水比l：4，pH值3.5条件下水解sh，水解率为46.37%，总氮回收率为87.94%，水解液呈较清澈的淡黄色；双酶采用先用2.6%风味酶水解3h后，再加入2.4%中性蛋白酶水解3h，水解率达44.57%，总氮回收率为86.95%。

ABSTRACT Bacillus subtilis tryptophanyl-tRNA synthetase was expressed and purified by the aid of the high-level expr...

利用枯草杆菌色氨酰tRNA合成酶的高表达质粒pKSW1表达和纯化了枯草杆菌色氨酰tRNA合成酶。

C, 200rpm for 5d then placed statically for more than 7d, and addition more than 0.01% Chitosan to Bacillus subtilis suspension provided significant UV protection. The survival spore rate is 90% about 2-2.5 fold better than control for spore exposed to UV radiation for 30 min. Bioassay in against Glomerella cingulata, and the inhibition activity is still 60%.

枯草杆菌於MIY液态培养基中，30°C，200rpm震荡培养五天后静置培养七天以上，以甲壳素添加浓度大於0.01％作为最适宜的紫外保护剂之作用最好，於UVB灯下暴晒30 min，对枯草杆菌孢子的保护效果最好，存活率达90％以上，抗紫外线活性是不添加紫外线保护剂的CK组的2.5-3倍，对檬果炭疽真菌仍有60%的抑菌活性。

Re-sults:it was shown that Nano MgO had excellent germicidal efficacy to the Escherichia coli and Staphylococcus aureus.To such spore as Bacillus subtilis var.niger spore,Nano MgO also produced good germicidal effect.It was proposed that abrasiveness,alka-linity,electrostatic attraction,and oxidizing power of nano MgO all combined to promote these biocidal properties.

结果： ①纳米氧化镁能有效杀灭大肠杆菌，对枯草杆菌黑色变种芽孢也有显著的杀灭效果；②纳米氧化镁对金黄色葡萄球菌和枯草杆菌黑色变种芽孢有很好的抑菌作用；③纳米氧化物的磨蚀性、碱性、氧化性以及对微生物的静电吸附使它具有抑菌和杀菌作用。

Results Synergized by microwave under the power of 210W, chlorhexidine and benzalkonium bromide represented weak activity for killing Bacillus subtilis var. niger, glutaraldehyde and ortho-phthalaldehyde presented obvious enhanced activity.

结果 在微波输出功率为210W的协同作用下，低效消毒剂显现微弱杀枯草杆菌黑色变种芽胞活性，高效消毒剂杀枯草杆菌黑色变种芽胞的活性则显著增强。

Subtilis chromosomal DNA. To the best of our Knowledge, this report is the first time to clone and express promotors from BCG in B.subtilis, and proved that the signal of transcription from BCG can be recognized by B.

就我们所知，我们的实验是首次将卡介苗的启动子在枯草杆菌进行克隆和表达，证明了卡介苗的启动信号也能为枯草杆菌所识别。

In view of these facts, We tried to find something in the L-form induction, plasmid extraction and their function, and the cloning and expression of promotors from BCG in Bacillus subtilis. Our aim is to provide a basis for the expression of HBsAg gene in BCG and to add something to the molecuiar biology of mycobacteria.

正是有鉴于此，我们试图在分枝杆菌在液体培养基中的L型实验诱导、质粒的提取及其功能分析、卡介苗启动子在枯草杆菌的克隆和表达等方面有所发现，为利用卡介苗表达乙型肝炎表面抗原基因提供基础，也为我国分枝杆菌的分子生物学研究增加内容。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失