枯草

The first method adopts different kind microbes to ferment buckwheatextraction. The method can increase DCI and its derivation content in buckwheat seed solution, while itreduces sugar content in extraction. The research adopts Saccharomyces cerevisia, Mucor, Bacterium coli, Torulopsis apicola, Aspergillus oryzae Bacillus subtilis, Candida mycoderma, Black mold that inoculate inbuckwheat extraction. Determine content change of various compotent with tracing.

方法一采用不同种类微生物发酵荞麦提取物，增加荞麦籽粒水溶液中D-手性肌醇及其衍生物的含量，同时减少提取物中总糖含量，本研究采用酿酒酵母、毛霉、大肠杆菌、蜜蜂生球拟酵母、米曲霉、枯草芽孢杆菌、解酯假丝酵母、黑曲霉接种于荞麦提取物中进行培养，并跟踪测定提取物中各种成分的含量变化。

LIFE CYCLE: Overwinter in thatch, behind leaf sheaths, or in stems.

生命周期：在枯草层，叶梢背面或者茎上面越冬。

Coli. The antimicrobial activity of the recombinant fusion protein was evaluated in vitro, which exhibited high antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, and Pasteurella multocida, but low antimicrobial activity against Escherichia coil, and no antimicrobial activity against Salmonell choleraesuis.

对该重组蛋白进行纯化与体外抗菌活性的测定。结果表明，重组鸡AvBD6融合蛋白对多杀性巴氏杆菌、金黄色葡萄球菌、枯草芽胞杆菌有较高抗菌活性，对大肠杆菌的抗菌活性较弱，对猪霍乱沙门氏菌无抗菌活性。

Coil and B. subtilis using the β-lactamase distribution assay. The results showed that the β-lactamase activities of E.coli are mainly in periplasm while the enzyme produced by B.

内酰胺酶活力测定表明，大肠杆菌产生的酶主要积累在周质空间，而枯草芽孢杆菌产生的酶主要分泌在胞外。

In the scorch stage, average yield of withered grass of second and third year growth prematurity type of Phleum pratense L. is 10672.5 kg/hm2, by cradling one time per year, average yield of wind hay grass is 7575 kg/hm2, the plant average height of selective colony is 160.0cm, the plant height of colony is rather equality. In the course of general nutrition composition analysis, discover that nutrition value of late-maturity type is higher than nutrition value ofprematurity type, coarse fibre and coarse protein and coarse fat of late-maturity type are 10.7%, 3.58%, 24.82% respectively, coarse fibre and coarse protein and coarse fat of prematurity type are 9.84%, 2.61%, 27.59% respectively, dry matter content of prematurity type is 92.63%, dry matter content of late-maturity type is 92.22%.

在枯黄期，第二、第三年生的早熟类猫尾草一年收割一次，枯草平均产量为10672.5 kg/hm~2，风干草平均产量为7575 kg/hm~2，选育出的群体平均高度为160.0cm，群体内植株株高较均一；在常规营养成分的分析中发现晚熟类的营养价值稍高于早熟类，晚熟类的粗蛋白是10.7％，粗脂肪是3.58％，粗纤维是24.82％，早熟类的粗蛋白是9.84％，粗脂肪是2.61％，粗纤维是27.59％，早熟类干物质含量92.63％，晚熟类干物质含量92.22％。

Objective: To explore the DNA sequence of the subtilisin-like protease (Pr1) gene of Pythium carolinianum, a mosquito-killing fungus.

目的：分离和克隆灭蚊真菌卡地腐霉类枯草杆菌蛋白酶基因已知片段的3'端未知序列。

The result expressed that under the concentration of 0.2mg/mL, the 6 compounds are all active to Sarcina lutea, ME-1, MT-6, MT-land MT-7 are active to Bacillus megaterium, to Escherichia coli, only MT-2 is active. The dimeric natural products are intensively existed in botany, most of them have the obvious biological activities. They have been concentrated on in recent years, and they are one of the compounds to which are enjoyed great popularity in natural product.

结果表明，在浓度为0.2mg/mL的条件下，六种化合物对藤黄八叠球菌均具有明显抑制作用，ME-1、MT-6、MT-1、MT-7对巨大芽孢杆菌有抑制作用，MT-2对大肠杆菌有抑制作用，对绿脓杆菌和枯草芽孢杆菌六种成分均无抑制作用；二聚体化合物在植物界广泛存在，多数二聚体天然产物具有显著的生物活性，引起人们的重视，是天然产物化学界研究的热门化合物之一。

Additionally, 75 volatile chemical constituents have been identified from the petroleum ether extracted part by capillary GC-MS, and the relative percentages of them are determined by GC method. The result showed that the volatile constituents of Musella lasiocarpa mainly includes advanced alkane, aliphatic acid and ester, aldehyde, ketone, aromatic and its heterocyclic compounds. With the compounds that have been isolated and identified from Musella lasiocarpa, the biogenensis pathway of new compound MT-3 is approached. Furthermore, the bacteriostasis of the 6 compounds to Escherichia coil, Pseudomonas aerusinosa, Bacillus megaterium, Sarcina lutea and Bacillus subtilic are determined by flat dilution method.

此外，从其石油醚萃取部分用毛细管色谱法分析鉴定出75种挥发性化学成分，并测定了各组分的相对百分含量，结果表明，地涌金莲挥发性化学成分以高级烷烃、高级脂肪酸酯及醛、酮、芳香和芳香杂环化合物为主；根据从地涌金莲中鉴出和分离得到的化合物，探讨了新结构化合物MT-3的生源代谢途径；采用平板稀释法测定了地涌金莲中六种化学成分对大肠杆菌、绿脓杆菌、巨大芽孢杆菌、藤黄八叠球菌和枯草芽孢杆菌的抑菌作用。

Strain NX-2 which can produceγ-glutamyltranspeptidase was isolated from the soils and identified taxonomically as Bacillus subtilis.

从土壤中筛选分离到一株产γ谷氨酰转肽酶的菌株，经生理生化特性鉴定为枯草芽孢杆菌NX 2。

The gene of transglutaminase was amplified from the chromosome of Bacillus subtilis DB403 by PCR and translationally fused with thioredoxin gene in the plasmid pET32a to achieve the expression vector pET32-BTGase.

利用PCR技术，从枯草杆菌DB403染色体上扩增出谷氨酰胺转胺酶基因，将其克隆到大肠杆菌载体pET32a中，成功构建谷氨酰胺转胺酶表达载体pET32-BTGase，并转化大肠杆菌BL21 (DE3)。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失