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The gas-liquid separator was constructed from a 30-mL borosilicate Midget Bubbler and a Teflon stopper with three holes.

气相离析器用30ml小型引水机和特氟隆三孔塞改造成。

Always use buttermilk, milk or sour cream at room temperature to prevent curdling and separation of the mixture.

Y( D5 Z 白脱奶、牛奶或酸奶油均应在室温下使用,以防止里面出现结块或离析。。

But all these test methods can not satisfy the requirements of the rapid development of highway construction due to their slow test speed, strong subjectivity, probable error, and destructivity to pavements.

这种状况与我国当前公路建设的发展很不适应,为此亟需发展连续、快速、无损的沥青混凝土路面离析检测技术。

Recently, the test and estimation of segregation in asphalt pavements mainly rely on traditional methods as visual identification, sand patch testing, ground-penetrating radar and coring. Due to some disadvantages of these test methods metioned, such as slow test speed, subjectivity, deviation and some destructivity.

目前,对沥青路面离析的判定和检测方法主要依赖于观察法、铺沙法、路面雷达法和取芯法,这些方法存在主观性强、测速慢和误差大,且具有一定的破坏性。

Essential branches or theories are composed of ethnomethodology,Bernstein's code theories,the sociology of language,the ethnography of speaking,discourse analysis,the social psychology of language,urban dialectology,social netw...

文章认为,今后一段时期内,西方社会语言学仍将是多元论和多样性的理论格局,离析论和综合论都将得到独自发展,但是,综合论将成为理论发展的总趋势。

Methods Paraffin and epidermis isolation methods were used.

方法采用石蜡制片和表皮离析方法。

But some kind of detects such as gapping, broaching, break, separation were usually found in bored cast-in-place pile, which maybe reduces bearing value of pile and does harm to the superstructure.

但是许多机械成孔的灌注桩常出现缩颈、扩颈、断裂、离析等缺陷,影响桩基承载力及上部结构的安全,严重者甚至使桩失去承载力。

It is a organic hexahydric weak acid. In different acidity, the dissociation degree and the color of reagent solvent were different also. Determined and draw the absorption curve of reagent in varies acidity, and figured out the dissociation constant by Perisic-Janjic method; the complex ratio of the reagent and bismuth was determinated by mole ratio method and continuous variation method; The apparent stability constant of the complex was calculated, the mechanism of dissociation and coordination were discussed.

2,6—二溴4—硝基偶氮氟胂是本实验室合成的测定铋的一个较好的试剂,对其进行了进一步的研究,在用柱层析法对其进行了提纯后,测定了试剂在不同酸华东师范大学硕:l:学位论文摘要度下的吸光度和最大吸收波长,绘制了其吸收曲线,用Perisic一Janjic法计算了试剂的六级离解常数;用摩尔比法和连续变化法测定了试剂与秘形成的配合物的络合比:计算了配合物的表观稳定常数,研究了其离解和配合机理,为筛选高效有机显色剂提供了理论依据。

Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.

针对本实验目的,我们设计了以下的实验步骤﹔(1)二次代谢物回收及检测样本处理:我们将发酵所得的培养上清液,利用疏水吸附性树酯Amberlite XAD-2吸附回收,并以甲醇溶离及真空减压浓缩脱水等方式处理,以取得提供抗氧化活性筛选之检测样本;(2)抗氧化活性检测及菌种筛选:以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用;经此筛选程序,我们选获了具有抗氧化物质高生产力的菌株,命名为AMBL-029C;(3)抗氧化物质的分离纯化:针对生产菌株的发酵回收处理液,以矽胶薄层色层分离法经物质层析纯化后,并以高效能液相层析法(High performance liquid chromatography;HPLC)分析物质可得一初级纯化物质,命名为AMBL-029C-TS;(4)抗氧化物质的物理化学性质分析:由酸碱转溶(acid-base fractionation)实验得知,此抗氧化物质属於中低极性的强酸性物质,对温度(37℃-100℃)及酸碱度(pH3.0-13.0)均表现出高稳定性;(5)在抗氧化机制探讨方面,我们针对数种不同的抗氧化机制进行探讨,即: 1。

The invention uses the fermentation liquor of one Escherichia coli generating polysialic acid as material, filters and removes thallus, adds some solvents as sodium chloride and alcohol into supernatant to deposit polysialic acid, dissolves the polysialic acid deposits via deionized water, filters to remove modified protein, uses alkali prolease to treat filtrate, complexes deposits via cetyl pyridinium chloride or cetyltrimethylammonium bromide, and dissociates, uses over alcohol to deposit polysialic acid, dissolves the deposits via ultra-pure water, grades and purifies via chromatography column, collects and dialyzes eluent, cools and dries to obtain polysialic acid.

本发明以一株产聚唾液酸大肠杆菌的发酵液为原料,经过过滤去除菌体,上清液加入适量氯化钠和乙醇等溶剂沉淀出聚唾液酸,聚唾液酸沉淀用去离子水溶解,再用过滤去除变性蛋白质,滤液经过碱性蛋白酶处理、氯代十六烷基吡啶或十六烷基三甲基溴化铵络合沉淀并解离后,再用过量乙醇沉淀出聚唾液酸,聚唾液酸沉淀用超纯水溶解,用层析柱进行分级纯化,收集洗脱液经过透析,冷冻干燥得聚唾液酸产品。

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