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The main research achievements on oogenesis and embryogenesis in mammals are described in this review,including the resumption of meiotic division;activation of MⅡ oocyte;development of parthenogenetic,gynogenetic,androgenetic and reconstituted embryos;and the genetic and reproductive potentials of chromosomes within the first and the second polar body.

本研究将论述哺乳动物卵子发生和胚胎发生的最新研究进展,包括:减数分列的启动, MⅡ期卵母细胞的激活,孤雌胚、雌核胚、雄核胚和重组胚的发育以及第一与第二极体染色体的遗传和生殖潜力。

Results In dioestrous mice, many iNOS+ cells were found in the stroma of endometrium. There was no obvious differences between the immunized mice and the control mice. In the uterus on 3 day of pregnancy, however, there was obvious difference about the iNOS quantity in uterus between the immunized and the control mice. There was strong positive reaction on the epithelia of endometrium and the endometrial gland in the lamina propria in control mice, while the staining of iNOS in the uterus of SA-30 immunized mice was very weak, only a few of iNOS+ cells were observed in the myometrium.

结果 SA-30免疫后对间情期小鼠子宫内的iNOS没有明显影响,无论是免疫组还是对照组,在子宫内膜基质中均有大量阳性细胞分布,两者无明显差异;在妊娠第3d时,对照组的小鼠子宫中有较多iNOS表达,阳性标记主要集中在内膜上皮及固有膜中的子宫腺上,经SA-30免疫的小鼠子宫中,iNOS表达则显著减少,整个内膜标记极弱,只在子宫肌层内有少量iNOS阳性细胞分布。

Results In dioestrous mice, many iNOS+ cells were found in the stroma of endometrium. There was no obvious differences between the immunized mice and the control mice. In the uterus on 3 day of pregnancy, however, there was obvious difference about the iNOS quantity in uterus between the immunized and the control mice. There was strong positive reaction on the epithelia of endometrium and the endometrial gland in the lamina propria in control mice, while the staining of iNOS in the uterus of SA-30 immunized mice was very weak, only a few of iNOS+ cells were observed in the myometrium.

结果 SA-30免疫后对间情期小鼠子宫内的iNOS没有明显瘙痒影响,无论是免疫组还是对照组,在子宫内膜基质中均有大量阳性偏瘫细胞分布,两者无明显瘙痒差异;在妊娠第3d时,对照组的小鼠子宫中有较多iNOS表达,阳性标记主要集中在内膜上皮及固有膜中的子宫腺上,经SA-30免疫的小鼠子宫中,iNOS表达则显著减少,整个内膜标记极弱,只在子宫肌层内有少量iNOS阳性偏瘫细胞分布。

Results:(1) After 24 h action of negative electrets, electrophoretic mobility and cell number in S phase of 3T3 cells were significantly increased compared with those in control group.

结果:(1)负极性驻极体作用成纤维细胞使细胞的电泳率和S期内的细胞数显著增加。

Breed can be used sow law, cuttage law and Qiegenfa to breed, sow ℃ of 20-25 of gemmiparous comfortable Wen Wei to control, spring, summer, Qiu Jun but, left and right sides of a month after sowing is gemmiparous, sow although the law is OK short time acquires more plant amount, but from the time that sows to blossom longer, still be much commonly so with cuttage breed is given priority to, cuttage with spring, summer two Ji Wei is comfortable period, insert with terminal bud for beautiful, cuttage sends a root extremely easily, cuttage hind can become a young plant about 4 weeks.

繁殖可利用播种法。扦插法及切根法繁殖,播种发芽适温为20-25℃左右,春。夏。秋均可,播种后约1个月左右发芽,播种法虽然可以短时间获得较多的植株数量,但是从播种到开花的时间较长,所以一般还是多以扦插繁殖为主,扦插以春。夏两季为适期,以顶芽插为佳,扦插极轻易发根,扦插后约4周即可成苗。

Under the condition of indoor storage the storage period of one month had not significant effect on germinative rate, but the storage period beyond two months, could have very significant effect.

在一般室内贮藏条件下,贮藏期1个月对小麦包衣种子的发芽率影响不显着,2个月以上,对包衣种子发芽率影响极显著。

It can be concluded that: First,primary deposit and placer deposit are two main deposits in this origin;Second,the primary deposit formed at hydrothermal stage and mesothermal stage between Presinian and Cretaceous;Third,the feature of ore body is wide and scattered on distributing,small on scale;Four,the storage capacity of the crystal is very abundant,its quality is fine,depth of burial is s.

通过对江苏东海区域成矿地质条件以及含晶石英脉成群成带分布规律的研究,得出以下结论:①该区水晶矿床为原生矿、砂矿两种类型;②水晶原生矿形成于前震旦纪以后到白垩纪以前的高、中温热液期;③矿体具分布广、规模小、分布散等特点;④东海水晶储量丰富、品位极高、埋藏浅,具有很好的开发利用前景。

Result Richards Model and Monodic Quadratic Equation could properly describe grain dry matter growing process and the change process of grain filling rate with days after anthesis. There was big difference in average grain filling rate, maximum grain filling rate under different planting density. Average grain filling rate 1.26 mg/ and maximum grain filling rate 2.44 mg/ of T7 were the quickest, and the average grain filling rate 0.94 mg/ and maximum grain filling rate 1.99 mg/ of T12 were the slowest, and the maximum difference percentage of the average grain filling rate and maximum grain filling rate among different density were 33.98%, 22.61%. There was significant correlation between average grain filling rate, maximum grain filling rate, grain filling active duration, rapid increasing stage and thousand grain weight, and the correlation coefficients were 0.628*, 0.630*, 0.849**, 0.739**. Active grain filling duration contributed mostly to TGW.

结果不同密度处理间千粒重、单位面积穗数、穗粒数、单株穗数、单位面积籽粒产量均存在显著差异;不同密度处理冬小麦的籽粒灌浆均符合慢-快-慢的&S&型生长特性,用Richards模型能很好地模拟冬小麦籽粒增重过程,用一元二次抛物线方程能较好地模拟冬小麦灌浆速率随花后时间变化过程;不同密度处理间平均灌浆速率、最大灌浆速率有较大差异,最大差异率分别为33.98%、22.61%,T7的平均灌浆速率1.26mg/及最大灌浆速率2.44mg/均最大,T12的平均灌浆速率0.94mg/及最大灌浆速率1.99mg/均最小;平均灌浆速率、最大灌浆速率、灌浆活跃期、灌浆快增期与千粒重显著或极显著正相关,相关系数分别为0.628*、0.630*、0.849**、0.739**;通径分析表明,灌浆活跃期对千粒重的贡献最大。

Basal leaves 2-6, long petiolate; stipules over 1/2 adnate to petioles, broadly lanceolate, membranous, margin sparsely denticulate and ciliate or entire, apex acuminate; petioles 3-9 cm at anthesis, usually green, sometimes purplish, glabrous, nitid, elongated at fruiting to more than 20 cm; leaf blade glabrous on both surfaces or pubescent along veins, 3-sect, segments shortly stipitate, lateral segments deeply 2-fid, central segment deeply 2- or 3-fid, terminal lobes highly variable in shape and size, ovate-lanceolate, lanceolate, oblong, or linear-lanceolate, margin irregularly dentate or shallowly divided, sometimes triparted to pinnatiparted or 3-lobed, apex obtuse or acute.

基生叶2-6,具长叶柄;托叶超过1/2的贴生至叶柄,宽披针形,膜,边缘疏生细齿和具缘毛或全缘,先端渐尖;叶柄3-9厘米在花期,通常绿色,有时略带紫色,无毛,有光泽的,在果期拉长到超过20厘米;叶片两面无毛沿脉具短柔毛,3全裂,裂片具短柄,侧裂片2深裂,中裂片2或3深裂,顶裂片形状和大小极易变,卵状披针形,披针形,长圆形,或线状披针形,边缘不规则牙齿或浅裂,有时3深裂到羽状深裂或3浅裂,先端钝或锐尖。

In situ hybridization using biotinylated POMC cRNA probe made it possible to localize POMC mRNA in the rat ovary. POMC mRNA predominantly existed in corpora lutea of diesturs, pregnant and pseudopregnant rat ovaries. POMC containing cells scattered in the ovarian intertitium were seen. The amount of POMC mRNA in granulosa cells was negligible.

采用生物素标记的POMCcRNA作为探针,进行原位杂文,研究POMCmRNA在大鼠卵巢中的分布,实验表明,POMCmRNA主要分布于间期,妊娠和假孕大鼠卵巢黄体中,间质细胞也可见少量POMCmRNA,而卵泡颗粒细胞中POMCmRNA极少。

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