条件分布

We introduced improved primary mixed glial culture and different-attachment method to isolate and purify the OPCs, the cells were proliferated in serum-free medium, flow cytometry and immunohischemistry methods were employed to estimate the purity of cultured OPCs. Their abilities of differentiation and expression of trophic factors were identified by RT-PCR and immunostaining. Several methods including TUNEL and MTT were adopted to estimate the protective effects of conditioned culture medium from oligodendrocyte lineage cells on the primary cultured cerebellar granule neurons. Intravitreal transplant of OPCs, combined with retrograde fluorescent labeling the superior colliculus and intraorbital optic nerve transection, were used to investigate the protective effects of OPCs on the axotomized RGCs in vivo. Intravitreal transplantof OPCs or NSCs on the newborn rats, and retinal transplant of OPCs on the young rats were performed, to observe the myelin formation in the retina at different stages after cellular transplantation. Optic nerve transection was carried out on some rats with myelinated retinae, to study the influence of myelination on the injuried RGCs.

为此，本研究采用改良的胶质细胞混合培养与差速贴壁方法获得大鼠OPCs，使用无血清培养基进行扩增、培养，用免疫组织化学和流式细胞技术对培养细胞的纯度进行鉴定，对少突胶质系细胞表达部分营养因子的情况进行检测；采用TUNEL、MTT等方法对少突胶质系细胞条件培养基对原代培养小脑颗粒神经元的保护作用进行检测；将OPCs移植入成年SD大鼠玻璃体内，利用上丘逆行荧光标记技术，观察眼内移植的OPCs对眶内视神经切断时的视网膜神经节细胞的保护作用及其持续时间；将OPCs或NSCs移植入新生和幼年SD大鼠玻璃体或视网膜内，观察不同时期视网膜内髓鞘形成与分布特点，分析髓鞘的超微结构，并观察眼内髓鞘形成对损伤神经节细胞的保护作用。

On the basis of intrapair and interpair correlation model, we have calculated the pair correlation energies of different kinds of systems, analyzed the changing rules of correlation energies of various electron pairs, and developed a reasonable and simple scheme called "Separating Large System into Smaller Ones". The basic idea of this scheme is that the correlation energy contributions of actual components in the ionic compound can be obtained using the correlation energy of ions with integer charge as the boundary conditions and incorporating the HF wave function or the distribution of electron cloud, then the total correlation energy of the system can be estimated by linearly summarizing the correlation contributions of these components.

在对内对间电子相关能理论模型的基础上，进行各类型化学键分子体系电子相关能的计算，分析电子对相关能的变化规律，建立合理的、&化整为零&的电子相关能简捷计算方案，其基本思想是对于离子键体系以整价离子的电子相关能为边界条件，通过HF波函数或电子云分布求得大体系中实际离子的电子相关能后，进行线性加和，即&化整为零、聚零为整&的思想。

Performance prediction of the pump when transporting water and salt solution is also studied.Results are compared and analyzed with experimental data.6.The salt-out flow field is measured on three dimensions by Particle Image Velocimemter via radial and axial two test models.After image processing, velocity distribution of crystal particles in the pump is obtained.Particle flow field is visualized to reveal the particle motion law intuitionally.

此外对泵送清水和盐溶液时的性能也进行了预测，并将结果与外特性试验数据对比。6、运用PIV在径向和轴向两种测量模式下对泵内不同外部条件下盐析两相流场进行了准三维测量，经图像后处理，获得了盐析晶体颗粒在叶轮内、无叶腔中及轴面上的速度矢量分布，实现了颗粒流场的可视化，更为直观地揭示了泵内盐析颗粒的运动规律。

The regularities of magnetic field spatial distribution of ironless PM motor with Halbach array are generalized. Thirdly, magnetic fields of different topology Halbach array motor are investigated comparatively by a large number of calculations.

利用渐近边界条件法，定量地计算了在定转子均无铁心的情况下电机内部及周围磁场的大小，总结出了Halbach阵列无铁心永磁电机磁场的空间分布规律。

The catalytic isomerization of isoborneol in the presence or Zn laden or Cu laden catalysts,in liquid phase or gas phase,is reported.

研究异龙脑在常压下被含锌或含铜催化剂催化的液相和气相异构化反应，讨论了反应条件对产物分布的影

The results show that the material achieves quite high thermal conductivity of 116 W/, when the carbon black is round and isolatedly distributed in the base material, and the soaking time is 4-6 h.

结果表明，以球形孤岛状分布的炭黑使材料具有较高的热导率，达到116W/；保温4~6h为较佳的工艺条件。

The results show that a few cavitations distribute randomly and isolatedly in the gauge length in Mg-8.42Li alloy at 573 K and 1.67×103 s1. The transgranular fracture appears at 573 K and 5×104 s1 in Mg-8.42Li alloy and dimple fracture along grain boundary appears at 573 K and 1.67×103 s1 in Mg-7.83Li alloy.

结果表明：Mg-7.83Li和Mg-8.42Li合金分别获得850%和920%的最大超塑性；Mg-7.83Li合金在573 K时发生了显著的超塑性晶粒长大；在573 K和1.67×103 s1条件下制备的Mg-8.42Li合金中的空洞较少，且在变形区中随机而孤立地分布。

6Al10Ti5 bulk amorphous alloy with a diameter of 3 mm were annealed isothermally for 600 s at 673 K and 823 K, for 1500 s at 823 K, and for 3600 s at 723 K.

6Al10Ti5块体非晶在不同退火条件下的显微组织与力学性能，等温退火后可以得到尺寸约为 30—60nm金属间化合物相弥散分布在非晶基体上的混合结构、研究表明：块体非晶的压缩屈服强度为 1。

In this experiment, typical ectomycorhizal fungi strains(Lactarius delicious 01, 02 and 03) were isolated from Three-Gorges Reservoir where Pinus Massoniana was very popular. And Suillus placidu 504(sp 504) and Laccaria bicolor S238N (Lb 23 8N) typical in South China were also chosen to study the effects of ectomycorrhizal fungi on the growth and nutrient absorption of Pinus Massoniana. And the culture characteristics of three strains of Lactarius delicious were also studied. The object of this study is to supply techniques in culturing ectomycorrhizal seedlings for planting trees in Three-Gorges Reservoir.

试验从马尾松大面积分布的三峡库区，分离出代表当地生态条件的松乳菇(Lactarius deliciosus 1、2和3)，并选择中国南方具有代表性的华南牛肝菌(Suillus placidu 504)和双色蜡蘑(Laccaria bicolor Lb S238N)，研究了松乳菇3个菌株的培养特性，并利用Lb S238N、Sp 504、Ld01和Ld03苗床接种马尾松，观察了它们对幼苗的生长和养分吸收的影响，目的是为三峡库区马尾松的植树造林提供培育菌根化苗木技术。

Lateritic is develop ed extensively in South China and Palaeoweather of South China was similar to that of Western Australia.

我国华南地区广泛分布有风化红土，这里的古地理和古气候条件与西澳耶尔刚地块相似，很有能在该区发现博丁顿型金矿。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。