杜松萜烯

ASES is 59% identical to Artemisia cyclase cDNA clone cASC125, 50% identical to epi-cedrol synthase from A. annua, 48% identical to amorpha-4, 11-diene synthase from A. Annua, 39% identical to the 5-epi-aristolechene synthase from tabacco, 38% identical to vetispiradiene synthase from H. Muticus, 41% identical to the δ-cadinene synthase from cotton. The coding region of cDNA was cloned into procaryotic expression vector pET30a and overexpressed in E. coli BL21 (DE3). The cyclase proteins extracted from bacterial culture were found largely in the insoluble protein fraction. The tissue specific expression patterns were analyzed by RT/PCR.

克隆的倍半萜合酶氨基酸序列与烟草马兜铃烯合酶、莨菪岩兰螺旋二烯合酶、棉花杜松烯合酶的一致性分别为39％，38％和41％；与青蒿柏木脑合酶、紫穗槐二烯合酶和一个推测的倍半萜合酶克隆cASC125的一致性为50％，48％和59％。cDNA编码区序列被克隆进原核表达载体pET-30a，并在大肠杆菌BL21（DE3）中诱导表达，但过量表达的蛋白主要是以不溶性蛋白形式存在。

The main volatile compounds were recorded, such as à-Cubebene (1.470%), Copaene (3.686%), C-Muurolene (3.152%, Eremophila-1(10), 11-diene (30.145%), Longifolene-(V4)(5.653%), Cadina-3, 9-diene (15.152%), Copaene (1.232%). The SPME fiber shows better selectivity and sensitivity toward some fragrance compounds comparing with other method.

主要挥发组分如：荜澄茄à-Cubebene (1.470%)、胡椒烯Copaene (3.686%)、衣兰油烯C-Muurolene (3.152%)、雅槛兰－1(10)，11-二烯Eremophila-1(10)，11-diene (30.145%)、长叶松萜烯Longifolene-(V4)(5.653%)、杜松－3，9-二烯Cadina-3，9-diene (15.152%)、古巴烯Copaene (1.232%)等。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。