杜什曼

To evaluate the specificity of the PCR, genomic DNA of Theileria annulata,Babesia bovis,Toxoplasma gondii,Leishmania donovani and standard strain of N. caninum were used as a template in the PCR. For determining the detection limit of amplification procedure, PCR was run on a dilution series of genomic DNA from N. caninum（1.562 5-200 ng/ml）. Brain tissue samples of 32 aborted fetuses were detected by PCR-based assay, and 23 blood samples from mothers were tested by ELISA. Results The amplified DNA fragment （350 bp）had a high identity of 98% with the Nc-5 gene sequence of N.

以环形泰勒虫、牛巴贝斯虫、刚地弓形虫、杜氏利什曼原虫以及犬新孢子虫标准株DNA为模板进行扩增以验证PCR的特异性，采用紫外分光光度计测定犬新孢子虫标准株DNA浓度和纯度，取高纯度的DNA样品用灭菌水稀释，分别取不同量的DNA进行PCR扩增，确定PCR方法的敏感性；利用该方法对32份奶牛流产的胎牛脑组织样品进行检测，同时，对其中23份流产的母牛血样进行ELISA血清学检测，以评价犬新孢子虫PCR方法的检测效果。

Therapontigonus Miles could walk arm in arm with Vadeboncoeur the grenadier, Damasippus the second-hand dealer would be happy among bric-a-brac merchants, Vincennes could grasp Socrates in its fist as just as Agora could imprison Diderot, Grimod de la Reyniere discovered larded roast beef, as Curtillus invented roast hedgehog, we see the trapeze which figures in Plautus reappear under the vault of the Arc of l'Etoile, the sword-eater of Poecilus encountered by Apuleius is a sword-swallower on the PontNeuf, the nephew of Rameau and Curculio the parasite make a pair, Ergasilus could get himself presented to Cambaceres by d'Aigrefeuille; the four dandies of Rome: Alcesimarchus, Phoedromus, Diabolus, and Argyrippus, descend from Courtille in Labatut's posting-chaise; Aulus Gellius would halt no longer in front of Congrio than would Charles Nodier in front of Punchinello; Marto is not a tigress, but Pardalisca was not a dragon; Pantolabus the wag jeers in the Cafe Anglais at Nomentanus the fast liver, Hermogenus is a tenor in the Champs-Elysees, and round him, Thracius the beggar, clad like Bobeche, takes up a collection; the bore who stops you by the button of your coat in the Tuileries makes you repeat after a lapse of two thousand years Thesprion's apostrophe: Quis properantem me prehendit pallio?

米勒会挽着侍卫华德朋克尔的胳膊，达马西普会在旧货店里流连忘返，万森刺杀苏格拉底正如阿戈拉囚禁狄德罗，格利木·德·拉雷尼埃尔会做油脂牛排正如古尔第吕斯发明烤刺猬。我们见到普劳图斯著作中的高架秋千重现在明星门的气球下面，阿普列乌斯在普西勒遇见的吞剑人便是新桥上的吞刀人，拉穆的侄儿和寄生虫古尔古里翁是一对，埃尔加齐尔请爱格尔弗依把他介绍给康巴色勒斯，罗马的四个纨袴子弟阿尔色西马尔古斯、费德洛木斯、狄阿波吕斯和阿尔吉里帕乘着拉巴突的邮车从拉古尔第①出发，奥吕·热尔在孔格利奥面前没有比查理·诺缔埃在波里希内儿面前待得更长久，马尔东不是母老虎，但是巴尔达里斯卡也绝不是一条龙，滑稽人潘多拉布斯在英格兰咖啡馆里嘲弄享乐人诺曼达纽斯，埃尔摩仁是爱丽舍广场的男高音，并且在他周围有无赖特拉西乌斯扮成波白什②向人募捐，在杜伊勒里广场上掐住你的衣扣、不让你走的那个讨厌人让你在两千年以后还重复着忒斯卜利翁的那句话："在我有急事时谁突然抓住了我的衣襟？"

AIM: To construct recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani anddetectexpressionofthegeneinNIH3T3cells.

目的： 构建杜氏利什曼原虫无鞭毛体蛋白编码基因的真核表达重组质粒pcDNA31amastin，并研究其在NIH3T3细胞中的表达。

METHODS: Amastin gene was amplified from nuclear DNA of Leishmania Donovani isolates and cloned into an eukaryotic expression vector pcDNA31.

目的： 构建杜氏利什曼原虫无鞭毛体蛋白编码基因的真核表达重组质粒pcDNA31amastin，并研究其在NIH3T3细胞中的表达。

Visceral leishmaniasis caused by Leishmania donovani is a serious disease andthe mortality is over 90 percent by estimation.

杜氏利什曼原虫(Leishmania donovani，L.d)引起的内脏利什曼病(visceral leishmaniasis，VL，又称黑热病)是利什曼病中症状最严重的一种类型，如不经治疗，病死率达90％以上。

This stuty consists of five parts: In the first part, amastin gene from DNA of two Leishmania donovani isolateswas amplified respectively by PCR. Amastin gene then was cloned into prokaryoticexpression plasmid pET-32a.

本研究共分为五部分：第一部分，以两株杜氏利什曼原虫基因组DNA为模板，通过PCR扩增获得了与预期大小一致、约552bp的杜氏利什曼原虫amastin基因。

Objective To explore the protein profile and identify developmentally regulated proteins of the promastigotes and axenic amastigotes with comparative proteomics technique.

摘要目的应用蛋白质组学技术分析杜氏利什曼原虫前鞭毛体和无鞭毛体蛋白质表达状况。

Therapontigonus Miles could walk arm in arm with Vadeboncoeur the grenadier, Damasippus the second-hand dealer would be happy among bric-a-brac merchants, Vincennes could grasp Socrates in its fist as just as Agora could imprison Diderot, Grimod de la Reyniere discovered larded roast beef, as Curtillus invented roast hedgehog, we see the trapeze which figures in Plautus reappear under the vault of the Arc of l'Etoile, the sword-eater of Poecilus encountered by Apuleius is a sword-swallower on the PontNeuf, the nephew of Rameau and Curculio the parasite make a pair, Ergasilus could get himself presented to Cambaceres by d'Aigrefeuille; the four dandies of Rome: Alcesimarchus, Phoedromus, Diabolus, and Argyrippus, descend from Courtille in Labatut's posting-chaise; Aulus Gellius would halt no longer in front of Congrio than would Charles Nodier in front of Punchinello; Marto is not a tigress, but Pardalisca was not a dragon; Pantolabus the wag jeers in the Cafe Anglais at Nomentanus the fast liver, Hermogenus is a tenor in the Champs-Elysees, and round him, Thracius the beggar, clad like Bobeche, takes up a collection; the bore who stops you by the button of your coat in the Tuileries makes you repeat after a lapse of two thousand years Thesprion's apostrophe: Quis properantem me prehendit pallio?

米勒会挽着侍卫华德朋克尔的胳膊，达马西普会在旧货店里流连忘返，万森刺杀苏格拉底正如阿戈拉囚禁狄德罗，格利木·德·拉雷尼埃尔会做油脂牛排正如古尔第吕斯发明烤刺猬。我们见到普劳图斯著作中的高架秋千重现在明星门的气球下面，阿普列乌斯在普西勒遇见的吞剑人便是新桥上的吞刀人，拉穆的侄儿和寄生虫古尔古里翁是一对，埃尔加齐尔请爱格尔弗依把他介绍给康巴色勒斯，罗马的四个纨袴子弟阿尔色西马尔古斯、费德洛木斯、狄阿波吕斯和阿尔吉里帕乘着拉巴突的邮车从拉古尔第①出发，奥吕·热尔在孔格利奥面前没有比查理·诺缔埃在波里希内儿面前待得更长久，马尔东不是母老虎，但是巴尔达里斯卡也绝不是一条龙，滑稽人潘多拉布斯在英格兰咖啡馆里嘲弄享乐人诺曼达纽斯，埃尔摩仁是爱丽舍广场的男高音，并且在他周围有无赖特拉西乌斯扮成波白什②向人募捐，在杜伊勒里广场上掐住你的衣扣、不让你走的那个讨厌人让你在两千年以后还重复着忒斯卜利翁的那句话：&在我有急事时谁突然抓住了我的衣襟？&

The Naqshbandiyya's role in the Panthay rebellion remains to be studied, but there are indications that Tu Wen-hsiu, who published the first complete Chinese translation of the Koran and adopted the title Sultan Sulayman, trying to create an independent sultanate at Tali in western Yunnan, may have been an initiate of Ma Ming-hsin's branch of the Naqshbandi mystical path.

纳格什班底耶在这次班赛起义中的作用还有待研究，但是杜文秀有可能是马明心的神秘主义道路分支的倡导者。杜文秀曾出版了第一本完整的汉译《古兰经》，而且使用的名字是&苏丹·苏来曼&。他曾试图在云南西部的大理建立一个独立的苏丹国。

Controverting the third or Roman theory of origin, he lays some stress upon the fact that Pope St. Innocent I (416) in his letter to Decentius of Gubbio spoke of usages which Mgr Duchesne recognizes as Gallican (eg the position of the Diptychs and the Pax), as "foreign importations" and did not recognize in them the ancient usage of his own Church, and he thinks it hard to explain why the African Church should have accepted the Roman reforms, while St. Ambrose himself a Roman.

Controverting第三或罗马起源的理论，他规定了一些压力的事实，教皇圣无辜口（ 416 ）在他的信中，以Decentius的古比奥以惯例的主教杜申承认高卢圣（例如的立场和Diptychs协会），作为&外国进口&，并没有认识到他们古老的使用自己的教会，他认为这很难解释为什麼非洲教会应该已经接受了罗曼改革，而他张永森圣罗马。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐