杆菌的

Different plant expression vectors had been respectively transferred into upland cotton cultivars Jinman12, Jinmian7, Xinluzao1 and Jihe321 via Agrobacterium tumfaciens transformation. These vectors carried cryIAc3 gene, which encodes Bacillus thuringiensis δ-endotoxin, under the control of chimeric OM promoter, CaMV 35S promoter, and cotton leaf curl virus RP promoter respectively; snowdrop lectin gene (Galanthus nivals agglutinin, gna) trans-regulated by rolC promoter controlled TGAla factor; and multivalent expression construct of both cryIAc3-cpti fusion gene and gna gene. A large number of transgenic plants and their progenies had been obtained.

为了提高外源基因的表达量，延缓害虫产生耐受性，本文通过将携带有分别在高效复合OM启动子，CaMV35S启动子及棉花曲叶病毒RP启动子控制下的苏云金杆菌杀虫毒蛋白基因cryIAc3；反式调节因子增强韧皮部表达的雪花莲外源凝集素(Galanthus nivals agglutinin，GNA)基因；以及同时含有cryIAc3、豇豆胰蛋白酶抑制剂基因和gna三价抗虫基因的植物表达载体，以根农杆菌介导法分别将这些表达载体导入了陆地棉新陆早1号、晋棉7号、晋棉12号和冀合321等我国西北棉花主要栽培品种，经体细胞胚再生获得了大批转基因再生植株。

The fungicidal activity of methanol extract was higher than that of ethylacetate extract. The bioassay showed that the methanol extracts of strain As fermentation products had certain degree fungicidical activity against Botrytis cinerea, Alternaria longipes, Glomerella cingulata, Curvulavia lunata, Gibberella zeae, Valsa malt under the concentration of 4000μg/mL, the inhibiting ratio against pathogen growth was ranging from 62.9%~85.1%; The results of the inhibition of spore germination indicated that the fermentation products exhibited obvious inhibition rate against Alternaria longipes, the EC50 values was 62.5328μg/mL; The bioassay showed that the methanol extracts of strain As fermentation products had certain degree fungicidical activity against Bacillus cereus , Bacillus subtilis.

其中甲醇提取物的抑菌活性较乙酸乙酯好，在4000μg/mL浓度下对番茄灰霉病菌、烟草赤星病菌、苹果炭疽病菌、玉米弯孢叫斑病菌、小麦赤霉病菌、苹果腐烂病菌6种植物病原真菌均有抑制作用，抑制率在62.9%~85.1%；抑制孢子萌发试验表明：菌丝体甲醇提取物对烟草赤星病菌的毒力较好，其EC50仅为62.5328μg/mL；抑制细菌活性表明菌丝体甲醇提取物对蜡状芽孢杆菌和枯草芽孢杆菌有一定的抑制作用，而对大肠杆菌无明显的抑制作用。

The pathogenic bacterium of prepartum and intrapartum infectious pneumonia mostly were G- bacilli,the pathogenic bacterium of postpartum infectious pneumonia mostly were G- bacilli and staphylococcus aureus.6.The drug resistant rates of G- bacteria to penicillins,cephalosporins,and monocyclicβ-lactam antibiotic were high,However,their compound of addingβ-lactamase inhibitor agents was relatively low resistance,and all G- bacteria were sensitive to carbapenem antibiotics.

产前及产时感染性肺炎的病原菌以G-杆菌多见，而产后感染性肺炎的病原菌主要为G-杆菌和金葡菌。6.G-菌对青霉素类、头孢菌素类及单环类的β内酰胺类抗生素耐药率高，但对其添加β内酰胺酶抑制剂的复合制剂耐药率较低，对碳青霉烯类抗生素均敏感；金葡菌对苯唑西林耐药率低，对万古霉素均敏感。7。

Secondly, Bifidobacterium medium was developed using JAJ as main material. The results showed that: Adding Tryptone,soy peptone.beef extract,com extract and Cys-HCl to JAJ could obviously promote the growth of Blm and Bbm;By the orthogonal experiment of three elements and three levels,a satisfying JAJ compound medium was acquired which included corn extract(0.3%), soy peptone(0.05%)and Cys-HCl(0.025%).Nextly,after establishing a selective Bifidobacterium medium, the effects of JAJ on the growth of Bifidobacteria in vivo were studied,using healthy mouse of Kunming species as experimental animal.

研究了以菊芋汁为主要原料的双歧杆菌培养基，大量试验结果表明，在菊芋汁中添加胰蛋白胨、牛肉膏、大豆蛋白胨、玉米浆和半胱氨酸盐酸盐等成分，对双歧杆菌有明显的促进生长作用；利用大豆蛋白胨、玉米浆和半胱氨酸盐酸盐设计了三因素三水平的正交试验，确定了菊芋汁复合培养基的优化配方：菊芋汁+0.3％玉米浆+0.05％大豆蛋白胨+0.025％半胱氨酸盐酸盐。

The specific PCR products of approximately 112bp in length was amplified from the other 10 strains of E.faecalis from lamb naturally infected with E.faecalis,lamb and mouse experimentally infected with E.faecalis by the PCR assay,but no any bands were amplified from Streptococcus equi subsp.equi,Streptococcus G,Streptococcus A,Staphylococcus aureus,Listeria monocytogenes from sheep,Mycoplasma ovipneumoniae and Brucella ovis.

应用该方法对分离的致羔羊脑炎粪肠球菌、由肠球菌导致发病或死亡羔羊脏器以及人工感染死亡小鼠的主要脏器进行扩增，均能得到特异性的DNA条带；对其他7种病原菌，马腺疫链球菌C群、G群链球菌、A群链球菌、金黄色葡萄球菌、羊源李氏杆菌、绵羊布氏杆菌、绵羊肺炎支原体不能扩增出特异的DNA条带。

Full enzymatic synthesis of N-protected Leu-enkephalin was successfully achieved via 3+2synthetic route by α-chymotrypsin and thermolysin, and the Z-protected Leu-enkephalin phenylhydrazine was obtained in tert-amyl alcohol by thermolysin for the first time.

以3+2的合成路线使用α-胰凝乳蛋白酶和嗜热杆菌蛋白酶完成了N-保护的亮-脑啡肽的酶法全合成，并在第三戊醇中首次以嗜热杆菌蛋白酶为催化剂得到了Z-保护的亮-脑啡肽酰苯肼衍生物。

The results indicate that (1) After acclimation and induction, Phosphorus Accumulating Organism not only has the characteristics of denitrification, but also has the characteristics of takeup phosphate in aerobic;(2) Denitrifying phosphate accumulating bacteria using NO3- as electron acceptor indeed existed in the conventional enhanced biological phosphorus removal system;(3) The facultative anaerobe seperated during the process of acclimation are Corynebacterium, Pseudodiphtheria corynebacterium, Staphylococcus and Moraxella; all of which can be functioned as denitrifying phosphorus accumulating to various degree.

试验结果表明：(1)好氧聚磷菌经驯化诱导后不但具有反硝化产氮特性，而且具有好氧聚磷特性；(2)在传统的A2O 工艺中存在有以NO3-为电子受体的反硝化聚磷菌；(3)在驯化过程中分离到的兼性厌氧微生物有棒状杆菌属、假白喉棒杆菌、葡萄球菌属及莫拉氏菌属，且都有不同程度的反硝化聚磷功能。

Bioleaching, by means of natural populations of Thiobacillus ferrooxidans and related Thiobacilli, for example, allows recovery of up to 70% of the copper in low-grade ores.

生物萃取，例如应用天然的氧化亚铁硫杆菌菌群和相关的硫杆菌，使低品级的矿石中铜的收率达到70％。

The main results are as follows:(1)In order to find out the catalysis character of pectinase and xylanse in bacillus circularise A_6 and exhibit their maxium catalysis rate,the optimum condition for determination of pectinase and xylanase activity in bacillus circularise A_6 was studied in aspect of pH,temperature,substance concentration,reaction time,anion concentration.

试验结果如下：(1)为探明环状芽孢杆菌果胶酶及木聚糖酶的催化特性，更好发挥它们的催化性能，本文从pH值、温度、底物浓度、反应时间、离子强度几个方面研究了环状芽孢杆菌A_6产生的果胶酶与小聚糖酶活性测定的最适方法。

Re-sults:it was shown that Nano MgO had excellent germicidal efficacy to the Escherichia coli and Staphylococcus aureus.To such spore as Bacillus subtilis var.niger spore,Nano MgO also produced good germicidal effect.It was proposed that abrasiveness,alka-linity,electrostatic attraction,and oxidizing power of nano MgO all combined to promote these biocidal properties.

结果： ①纳米氧化镁能有效杀灭大肠杆菌，对枯草杆菌黑色变种芽孢也有显著的杀灭效果；②纳米氧化镁对金黄色葡萄球菌和枯草杆菌黑色变种芽孢有很好的抑菌作用；③纳米氧化物的磨蚀性、碱性、氧化性以及对微生物的静电吸附使它具有抑菌和杀菌作用。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。