杆菌的

Strains containing cry1 genes were the most abundant in our collection (66%), and 21 different cry1-type gene combinations were found. Furthermore, several novel holotype cry genes were found and the full-length sequences of 3 novel cry genes were designated as cry54Aa1, cry30Fa1, and cry30Ga1 by B.

PCR-RFLP鉴定结果表明：此地区的苏云金芽胞杆菌主要含有cry1，cry2，cry3，cry4/10，cry9，cry30和 cry40 等7种cry基因类型；含cry1基因的菌株最丰富，共有21种不同cry1型基因组合；从中发现了新型模式基因，并采用Tail-PCR技术获得了其中3个基因的全长序列，被国际苏云金芽胞杆菌杀虫晶体蛋白基因命名委员会命名为cry54Aa1、cry30Fa1和cry30Ga1。

In the experiment, two partly modified insect-resistant genes were transferred to the triploid hybrid of Chinese white poplar (Populus tomentosa Carr.譖opulus bolleana Louche譖opulus tomentosa Carr.

本项目研究了用部分改造的 BtCry1Ac基因与慈菇蛋白酶抑制剂基因构建的双抗虫基因表达载体通过农杆菌介导法对三倍体毛白杨的转化及对转化后植株体内残存农杆菌在继代培养和移栽过程中进行了跟踪检测。

Diphtheroids were found on 80% of keyboards, Micrococcus species on 72%, Bacillus species on 64%, nonfermentative Gram-negative rods on 36%, vancomycin-sensitive Enterococcus on 12%, Staphylococcus aureus on 4%, and oxacillin-susceptible S aureus on 4%.

在所有键盘中都发现了最常见的致病菌凝固酶阴性葡萄球菌。80％的键盘带有类白喉菌，72%带有微球菌属，64%带有芽胞杆菌属，36%带有非发酵的革兰氏阴性杆菌，12%带有万古霉素敏感肠球菌，4%带有金黄色葡萄球菌，4%带有甲苯异恶唑青霉素易感链球菌。

Any of various rod-shaped, nonmotile aerobic bacteria of the genus Lactobacillus that ferment lactic acid from sugars and are the causative agents in the souring of milk.

乳酸杆菌任一种杆状的乳杆菌属的不游动好氧菌，它能使糖发酵产生乳酸，也是牛奶发酸的导因

Any of various rod - shaped,nonmotile aerobic bacteria of the genus Lactobacillus that ferment lactic acid from sugars and are the causative agents in the souring of milk.

乳酸杆菌任一种杆状的乳杆菌属的不游动好氧菌，它能使糖发酵产生乳酸，也是牛奶发酸的导因非生□，抗热□，非机体酵素

We also compare the correlation between normal PCR bifidobacteria quantification and traditional diluting bifidobacteria quantification.

并且比较普通PCR定量的双歧杆菌结果和传统稀释滴种法定量的双歧杆菌结果的相关性。

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

Compared with 11 other isolates reported in GenBank, there was slightly variation in the nucleotide sequence of Omp25 gene of wapiti's isolate. The identities of nucleotide sequence and deduced amino acid sequences among 11 strains were 99.1%-99.4% could 99.2%-99.5% respectively.

与GenBank中已经登录的布氏杆菌其他11个分离株相比，马鹿布氏杆菌分离株Omp25基因变异较小，以散在的点突变为主，Omp25基因核苷酸和推导氨基酸序列的同源性分别为99.1%-99.4%和99.2%-99.5%之间。

OBJECTIVE To detect the effects of DNA from actinobacillus actinomycetemcomitans associated with periodontal disease for the immune response to other bacterial antigens in the oral cavity, and to study the feasibility of DNA from actinobacillus actinomycetemcomitans associated with periodontal disease as adjuvant of oral vaccine.

［目的］测定牙周病相关细菌伴放线放线杆菌DNA对口腔内其他细菌性抗原的免疫应答的影响，并探讨牙周病相关细菌伴放线放线杆菌DNA作为口腔疫苗佐剂的可行性。

It is investigated that include autolytic activity in LB-3 culture medium,the relationship between cell dead and autolytic activity as well as the effect of temperature.

结果表明，乳酸杆菌LB-3的自溶酶系在SDS-PAGE上呈现出两个区域，即乳酸杆菌LB-3的自溶酶系至少由两种分子量不同的酶系组成。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。