杆菌的

The invention discloses a preparing method of bacillus alcaligenes strain Alcaligenes sp., CCTCC No.M206121 of degraded o-nitrophenol, which comprises the following steps: allocating inorganic salt culture medium with dibasic sodium phosphate, monobasic potassium phosphate, manganese sulfate, copperas, addex-magnesium, calcii chloridum, blue copperas, white copperas and hydrogen dioxide solution; adjusting pH value; sterilizing under high temperature; enriching bacterial; sampling from sludge; allocating suspending liquid with soil sample and inorganic salt; seeding into ortho-nitrophenol fresh organic salt culture medium with finite volume seeding quantity; keeping the temperature; culturing on shaker; getting enriched culture; separating and purifying enriched culture in LB solid culture medium.

本发明公开了一种降解邻硝基酚的产碱杆菌菌株及制备方法，产碱杆菌为Alcaligenes sp。，CCTCC No.M206121。其制备步骤是：首先是配制无机盐培养基，成分为磷酸氢二钠、磷酸二氢钾、硫酸锰、硫酸亚铁、硫酸镁、氯化钙、硫酸铜、硫酸锌和双蒸水，调pH值高压灭菌；其次是菌种的富集，从污泥中取样，将土样与无机盐培养基配制成悬浮液，将悬浮液以一定体积接种量接种到含邻硝基酚的新鲜无机盐培养基中，恒温，摇床培养，获富集培养物；第三是将富集培养物在LB固体培养基中分离和纯化。

Agrobacterium A genus of soil bacteria, the species A. tumefaciens being the causative agent of CROWN GALL, a type of tumor in plants.

根癌农杆菌：土壤中存在的一种农杆菌，可以感染双子叶植物的受伤组织诱发一种植物的肿瘤－冠瘿瘤。

Using the cooperations between sporulation and competence-forming related genes both from other pleitropic regulatory genes of Bacillus, hpr, degQa, degU/S 32and degR and from the Bacillus subtilis Ki-2-132. The series high-expressive genetical engineering host strains were constructed.

利用芽孢杆菌多效调控基因hpr、deqQa、degR、degU／532等和枯草杆菌Ki－2－132自身的积生孢、感受态相关基因的协调作用，构建了系列的高表达遗传工程宿主。

With the competent germinating epicotyl of cotton as receptor in exogenous gene transformation, NPTII (neomycin phosphotransferase Ⅱ) gene carrying selection marker and GFP reporter gene were mediated into cotton by agrobacterium-mediated transformation. The pre-culture time, bacterial-immersing time, co-culture time and antibiotics concn., etc of using agrobacterium-mediated transformation in the transformation of competent germinating epicotyl of cotton were studied.

方法］以棉花感受态萌动的上胚轴作为外源基因转化的受体，用农杆菌介导法将携带有筛选标记NPTII基因及GFP报告基因导入棉花，研究了农杆菌介导法用于棉花感受态萌动上胚轴转化的预培养时间，浸菌时间，共培养时间及抗生素处理浓度等。

MethodWiththe competent germinating epicotyl of cotton as receptor in exogenous gene transformation,NPTIIgene carrying selection marker and GFPreporter gene were mediated into cotton by agrobacterium-mediated transformation.The pre-culture time,bacterial-i mmersing ti me,co-culture ti me and antibiotics concn.,etc of using agrobacterium-mediated transformationinthe transformation of competent germinating epicotyl of cotton were studied.

方法以棉花感受态萌动的上胚轴作为外源基因转化的受体，用农杆菌介导法将携带有筛选标记NPTII基因及GFP报告基因导入棉花，研究了农杆菌介导法用于棉花感受态萌动上胚轴转化的预培养时间，浸菌时间，共培养时间及抗生素处理浓度等。

Objective To explore the effect of Lactobacillus fermentation filtrate and its components on the cell cycle and apoptosis of Hela cell as well as its possible mechanism.

目的 观察乳酸杆菌DM9811发酵滤液对宫颈癌细胞株Hela细胞的细胞周期和细胞凋亡的影响，探索乳酸杆菌发酵滤液对宫颈癌细胞作用的可能机制。

The plantlets of soybean, cucumber and garden balsam were inoculated by wild-type Agrobacterium rhizogenes K599, and hairy root was induced on inoculated sites in vivo. The frequencies of hairy root induction from wound cotyledons of soybean, cucumber and garden balsam were 100%, 65% and 91%, respectively.

利用野生型的发根农杆菌K599对属于不同科属的大豆、黄瓜和凤仙花进行活体感染实验，结果表明，发根农杆菌K599可使切割后的大豆、黄瓜和凤仙花子叶形成不定根，生根频率分别为100%、65%和91%。

Because of the characteristics of tiny ecoenvironment under the gums, gums next stir up an in great quantities poisonous dint's bigger tooth week to the germ in the germ spot, like gum rod bacteria, in the center type rod bacteria, helicoid etc., make the burning disease of gum aggravated and expand to postpone, cause tooth for week bag formation and the tooth slot bone absorption, make tooth burning for week.

由于龈下微生态环境的特点，龈下菌斑中滋生着大量毒力较大的牙周致病菌，如牙龈类杆菌，中间类杆菌，螺旋体等，使牙龈的炎症加重并扩延，导致牙周袋形成和牙槽骨吸收，造成牙周炎。

Haemophilus parasuis disease caused by the HPS is also designated as Haemophilus pig disease, Glaser Glaser's disease or Gram's disease (Glasser's disease) and characterized by multiple serositis and arthritis.

副猪嗜血杆菌（ Haemophilus parasuis ， HPS ）病也又称猪副嗜血杆菌病、格拉泽氏病或革拉泽氏病(Glasser's disease)，是由HPS引起的猪的以多发性浆膜炎和关节炎为主要特征的一类疾病。

With metabolic pathway analysis method, the biosynthesis pathway of guanosine by Bacillus subtillis in steady state was analysed in this paper, and three basic models were established, and th.

利用途径分析方法对枯草芽孢杆菌生产鸟苷的途径进行了分析，建立了 3种基础模型，鸟苷理论摩尔产率分别是 0 6 2 5、0 75和 0 6 6 7，确定了枯草芽孢杆菌生产鸟苷的最佳途径的通量分布。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。