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The gene of ESAT-6 from genome of bovine mycobacterium tuberculosis strain that from cervine tuberculosis was amplified by polymerase chain reaction and inserted into pGEM-TE vector. After sequencing, we analyzed the gene encoding ESAT-6 protein with molecular biologic software.

从患结核病鹿的肺组织中粗分离牛分支杆菌制备DNA模板,用PCR对牛分支杆菌ESAT-6基因进行了扩增,将扩增的产物连接于测序载体pGEM-TE上克隆,经测序确定无误;并使用分子生物学软件对ESAT-6基因进行了分析。

Three kinds of plant expressing vector pC1304- CamV35S+ Cbcor15b, pC1304-cold-induced promotor+ Cbcor15b*, pC1304- CamV35S+ Cbcbf for transforming dicotyledon plants was constructed successfully by substituting the report gene of pC1304 with the coding sequence of our destination gene. The recombinant vector was introduced into Agrobacterium tumefaciens with freeze-thaw method. The leaf discs of tobacco plants infected by A. tumefaciens were selected with hygromycin. The regenerated plants were tested by PCR.

在成功的构建pC1304- CamV35S+Cbcor15b,pC1304-冷诱导启动子+Cbcor15b*,pC1304- CamV35S+ Cbcbf三种植物表达载体之后,采用冻融法成功地将重组质粒转化到了根癌农杆菌菌株EHA105中,采用农杆菌介导的烟草叶盘转化法,将荠菜Cbcor15b,Cbcor15b*,Cbcbf,转入模式植物烟草中,在诱导生根后对转基因苗进行了PCR检测。

The size of bacteria,characteristic of stain,move about under light microscope and types of endospore were observed.

通过观察实验菌株的形态、染色特征、动力、芽孢的形态与位置,结合葡萄糖、阿拉伯糖、乳糖、肌醇发酵、卵磷脂试验、尿素水解试验、溶血性试验、青霉素抗性试验、溶菌酶抗性试验等,结果3种微生物被鉴定为蜡样芽孢杆菌、克柔念珠菌、枯草芽孢杆菌。

objective to study the curative effect of fulguration combining mycobacterium injection in treat ment of condyloma acuminatum.methods the patients with ca were treated by fulguration in large range and local mycobacterium injection under fulguration wound.results the recurrence rates were 20.59% and 58.82% respectively in the observation group and in the control group.

目的 探讨电灼联合局部注射草分枝杆菌治疗尖锐湿疣的效果。方法治疗组应用电灼大范围炭化疣体,并在创面即疣体的基底部注射草分枝杆菌。对照组单纯采用高频电灼治疗。结果两组愈后复发率分别为20.59%、58.82%,前者明显较后者低,两组比较差异有非常显著性( p <0.01)。

All resonance peaks of gramicidin S were assigned.And then,the NMR assignments were used to determine the three dimensional structure of gramicidin S in DPPC reversed micelle.

用二维相关谱和二维NOE谱对反向脂囊泡中短杆菌肽S的共振峰进行了识别,短杆菌肽S所有的共振峰都被指定。

Nutrients are cultured in organic media that may contain: organic milled soy, organic yeast a natural source of PABA and SOD, organic maltodextrin, organic gum acacia, organic orange and lemon peel - natural sources of bioflavonoids, organic carrot powder, organic alfalfa powder, enzymes and Lactobacilli (L.acidophilus, L. bifidus, L.rhamnosus).

养分培养有机媒体可能包含:有机米酱油,有机酵母的天然来源,苯甲酸和超氧化物歧化酶,有机麦芽糊精,有机阿拉伯胶,有机橙和柠檬皮-天然来源的生物类黄酮,有机胡萝卜粉有机苜蓿粉,酵素和乳酸菌(嗜酸乳杆菌,双歧杆菌属, L.rhamnosus )。

Dagger; Nutrients are cultured in organic media that may contain: organic milled soy, organic yeast a natural source of PABA and SOD, organic maltodextrin, organic gum acacia, organic orange and lemon peel - natural sources of bioflavonoids, organic carrot powder, organic alfalfa powder, enzymes and Lactobacilli (L.acidophilus, L. bifidus, L.rhamnosus).

dagger;养分培养有机媒体可能包含:有机米酱油,有机酵母的天然来源,苯甲酸和超氧化物歧化酶,有机麦芽糊精,有机阿拉伯胶,有机橙和柠檬皮-天然来源的生物类黄酮,有机胡萝卜粉有机苜蓿粉,酵素和乳酸菌(嗜酸乳杆菌,双歧杆菌属, L.rhamnosus )。

Any of various rod-shaped, nonmotile aerobic bacteria of the genus Lactobacillus that ''.

乳酸'。''杆菌任一种杆状的乳杆菌属的不游动好氧菌,它能使糖''。

The genus Mycobacterium consists of nonmotile,nonspore - forming aerobic bacilli,(0.2~0.6) m m ×(1~10) m m in size.

分枝杆菌属是一类细长或稍弯的杆菌,因有分枝生长的趋势而得名。

Moreover. These results would be helpful for future research on pathogenicity of Shigella spp.

这些结果加深了我们对痢疾杆菌中H-NS蛋白的认识,有助于痢疾杆菌致病机理的研究。

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