杆菌的

in order to establish a rapid, sensitive and specific detection method for identification of four kinds of food-borne pathogenic bacteria including salmonellla spp, listeria monocytogenes, staphylococcus aureus and bacillus cereus by using multiplex pcr, according to invasion protein a geneof salmonellla spp, transcriptional regulatory protein gene of listeria monocytogenes, autolysin gene ofstaphylococcus aureus and gyrase b subunit gene of bacillus cereus, four pairs of specific primers were designed for multiplex pcr amplification.

小 摘要：为建立一种利用多重pcr技术检测和鉴定沙门氏菌、单核细胞增生李斯特菌、金黄色葡萄球菌和蜡样芽孢杆菌的方法，根据沙门氏菌侵袭力蛋白a基因、单核细胞增生李斯特菌蛋白转录调控基因、金黄色葡萄球菌自溶素基因、蜡样芽孢杆菌的促旋酶b亚单位基因设计引物，进行多重pcr扩增，并对多重pcr反应体系进行优化。

The detection limits were 0.05μg/ml for cadaverine,histamine and spermidine, 0.1μg/ml for tyramine, 0.25μg/ml for spermine. The recoveries rate of the method were 96.6%,101.94%,101.90%,98.65%,107.4% for cadaverine, histamine, tyramine, spermidine and spermine, respectively.

通过在发酵香肠中强化接种弯曲乳杆菌6031和肠膜明串珠菌51-5 D单一和混合菌种，使用高效液相色谱法检测了7种生物胺含量的变化，同时进行乳酸菌、球菌、总细菌、肠杆菌的计数。

This finding does not support a significant role for Mycobacterium avium subspecies paratuberculosis in the pathogenesis of Crohn's disease in the majority of patients.

这个发现也不支持鸟分支杆菌的亚型副结核分支杆菌是大多数克隆氏病患者的病因。

This finding does not support a significant role for Mycobacterium aium subspecies paratuberculosis in the pathogenesis of Crohn's disease in the majority of patients.

这个发现也不支持鸟分支杆菌的亚型副结核分支杆菌是大多数克隆氏病患者的病因。

B-type was the main serotype of Pasteurella isolated from Zebra, Sambar and White-lipped deer. A-type was the main serotype of Pasteurella isolated from Red Kangaroo.

其中荚膜B型是斑马、黑鹿和白唇鹿源多杀性巴氏杆菌的主要血清群，荚膜A型是赤大袋鼠源多杀性巴氏杆菌的主要血清群。

Objective To investigate the feasibility of multiplex PCR for rapid identification of Mycobacterium tuberculosis and non-tuberculosis Mycobacteria.

目的 探讨应用多重PCR技术快速鉴定结核分枝杆菌与非结核分枝杆菌的可靠性。

To set up a quantitative suspension mycobactericidal test, and study the efficacy of 6 frequently used disinfectants against mycobacteria.

目的： 1。建立分枝杆菌定量杀灭试验方法，研究常用消毒剂对分枝杆菌的杀灭效果。

Objective To establish the PCR method for detection of Helicobacter pylori DNA in tissue embeded by paraffine and to investigate the relationship between Hp and recurrent oral ulcer.

目的 建立检测石蜡切片中幽门螺杆菌的PCR方法，明确复发性口腔溃疡患者口腔中是否存在幽门螺杆菌。

This method could amplify the specific fragment in the reference strains, but not in chicken E. coli, Haemophilus paragallinarum, Salmonella Pullorum and Avian pasteurella multocida, of ORT. A sensitivity test indicated that the assay's lowest detection limit was 90 pg DNA.

该方法能在ORT参考菌株中扩增到特异性片段，而鸡大肠杆菌、副鸡嗜血杆菌、鸡白痢沙门氏菌、禽多杀性巴氏杆菌的扩增结果均为阴性；敏感性试验表明该方法最低检出限量为90 pg DNA。

Meanwhile, a new biger genetic group containing 6 cilicate sWains was formed, and it was different from the reference strains. The phylogenic tree of the representative sWains HLJ01 (GenBank accession EF031269) and HB 16 (GenBank accession EF031270) was constructed by analysis of the 16S rDNA full sequence, the results of analysis of 16S rDNA sequence revealed that swain HLJ01, HB 16 and Bacillus mucilaginosus and Bacillus edaphicus were located in the same phylogenic branch. HLJ01 and HB16 had high homology with Bacillus mucilaginosus in the phylogenic tree, but the 16S rDNA sequence of HLJ01 only has the identity of 96.7% with Bacillus mucilaginosus, and of HB16 has the identity of 95.5% with Bacillus mucilaginosus.

根据16S rDNA-RFLP聚类结果，选取供试硅酸盐细菌菌株HLJ01和HB16作为代表测定了其16S rDNA全序列，其Genbank序列登录号分别为EF031269和EF031270.16SrDNA序列分析表明：代表菌株HLJ01与HB16虽然与胶质芽胞杆菌位于同一个系统发育分支，但相似性分别只有96.7％、95.5％；与土壤芽孢杆菌的相似性则分别为94.6％和93.3％。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。