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Experiments were carried out to study minimum bactericidal concentration of 5 kinds of disinfectants (double fouthdiamine sait-iodine solution, compound aldehyde, chlorine-containing disinfectant; sodium hydroxide and potassium permanganate) against 5 kinds of pathogenic bacteria, and the above MBCs of disinfectants were 1: 600-1: 800,1:1000-1: 2500,1: 30000-1: 40000,0.01%-0.03% and 0.0008%-0.004%, respectively. It emphatically evaluated the sterilized efficacy of each kind of disinfectant to salmonella at different temperatures.

为了解目前猪场常用消毒剂对猪场常见肠道致病菌的杀菌作用,开展了5种猪场常用消毒剂(特碘、复合醛、消特灵、氢氧化纳和高锰酸钾)杀灭5种猪场常见肠道致病菌(沙门氏菌、大肠杆菌、奇异变形杆菌、弗氏拘橼酸杆菌和绿脓杆菌)最低浓度的试验,依次为l:600~l:800,1:1000~l:2500,l:30000~l:40000,0.01%~0.03%,

Any of various gram-negative, rod-shaped bacteria of the genus Proteus, certain species of which are associated with human enteritis and urinary tract infections.

变形杆菌任何一种革兰氏阴性的杆状变形杆菌属细菌,某些变形杆菌与人类的肠类和尿道感染有关注意,注意力集中

The optimum pH value was 9.5, cane sugar of carbon source, yeast extract of nitrogen source, and sodium nitrate were conducive to enzyme production.

目前用于碱性蛋白酶的生产菌和研究对象主要是枯草芽孢杆菌和地衣芽孢杆菌及短小芽孢杆菌,新的产酶菌株目前

Seven major bacterial isolates were obtained and purified. Their 16S rRNA genes were amplified by PCR for sequencing and their identities were determined with homology comparisons. Five of the seven isolates belong to Actinomycetales including Kocuria KD139, Rhodococcus KD140, Rhodococcus KD142, Arthrobacter KD230, and Arthrobacter KD232; one is classified as Bacillus d KD178; and another one as Stenotrophomonas KD237. The phylogenetic tree was also constructed based on the analysis of 16S rRNA gene sequences.

通过对分离菌株的16S rRNA基因序列进行分析,发现其中5株细菌分别属于放线菌目的考克氏菌属(KD139)、红球菌属(KD140和KD142)和节杆菌属(KD230和KD232), 1株细菌属于杆菌目的芽胞杆菌d属(KD178),另外1株细菌属于黄色单孢菌目的寡食单胞菌属(KD237);同时我们构建了系统进化树,确定分离菌株的相对进化地位。

According to the identification, Bacillus subtilis CGMCC No.1982 is a typical Bacillus subtilis. Compared with other Bacillus subtilises, Bacillus subtilis CGMCC No.1982 has broad-spectrum antipathogen fungal activity; the fermentation broth and spore have sound effect in preservation of fresh fruits such as peach, grapes, etc, and also have sound antiviral effect in production of cucumber, tomato and other greenhouse vegetables.

经鉴定枯草芽孢杆菌CGMCC No.1982属于典型的枯草芽孢杆菌,与其它枯草芽孢杆菌相比其具有广谱抗病原真菌活性,其发酵液和芽孢对鲜桃、葡萄等水果保鲜效果较好;用于黄瓜、番茄等大棚蔬菜的生产抗病效果较好。

In order to investigate new methods to the detection of Mycobacterium tuberculosis and diagnostic value of FQ-PCR in the detection of DNA in Mycobacterium tuberculosis, FQ-PCR was used to detect DNA of Mycobacterium tuberculosis in body fluid from 68 patients with tuberculosis and 68 patients without tuberculosis and it was also compared with the two methods of smear acid-fast stainning and antituberculotic antibody.

为探讨结核分枝杆菌检测的新途径及其FQ-PCR技术在结核分枝杆菌DNA诊断中的实用价值,运用FQ-PCR技术对68例结核病患者和68例非结核病患者体液进行结核分枝杆菌DNA测定,并与涂片抗酸染色及ELISA—抗结核抗体法进行比较。

In order to analyze the specificity of multiple PCR, multiple PCR detection system was used to detect Blastomyces albicans, Chlamydiae trachomatis, Escherichia coli, hemophilies influenzae, Klebsiella pneumoniae, Staphylococcus aureus, Streptococcus pneumoniae, Human cytomegalovirus, duovirus, adenovirus and Henrietta Lacks strain of cancer cells.

用此多重PCR体系分别检测分离的白色念珠菌、沙眼衣原体、大肠埃希氏杆菌、流感嗜血杆菌、肺炎克雷伯杆菌、金黄色葡萄球菌、肺炎链球菌、人巨细胞病毒、轮状病毒、腺病毒和Hela细胞,分析此多重PCR体系的特异性。

The main results are illustrated as following: 1. With the probe of the argB gene from Corynebacterium glutacium ATCC13032, it was made sure that the argB genes between C. crenatum A. S 1. 542 and C.

主要研究结果如下: 1 通过Southern Blotting实验,以谷氨酸棒杆菌ATCC 13032的argB基因为探针,确定钝齿棒杆菌A.S.1.542与谷氨酸棒杆菌ATCC 13032有较高的同源性。

Bacteriological examination, Pasteurellosis dye concentration polarization can be detected in the bacteria, but this disease can be detected in a similar kind of Brevibacterium sp.

细菌学检查,巴氏杆菌病可检出两极浓染的杆菌,而本病可检出类似球菌样的短杆菌。

And the transformation efficiency of Enterococcus faecalis is obviously higher than that of Lactobacillus plantarium by pulse electroporation with different voltages, which suggests that the two strains of LAB have different usage in the research of genetic engineering of LAB.

经过16SRNA全长序列分析及配合生理生化实验鉴定,结果显示球菌为肠球菌属中的粪肠球菌,杆菌为乳酸杆菌属中的植物乳杆菌,是两株胃肠道内正常定植菌。

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