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And unrooted trees were established. The accession numbers in GenBank of the sequences of 16S rDNA of the strains were followed by PFK1(DQ295034), PFK2(DQ295035), PFK3(DQ295036), LZCL(DQ295040), ZCS1(DQ295037), MMA1(DQ295041), MMA2(DQ295038), JSQ1(DQ295042), JSQ2(DQ295039), JSQ3(DQ295043). The Blast and phylogenetic tree analysis showed that

3将分离菌株进行形态、生理和糖发酵实验,结果表明这些表型鉴定的结论与16 S rRNA基因序列同源性分析结论一致,分离纯化5种产品的10株菌中仍有相同的3株菌与标签标注的不一致,即其中标签上分别标注为青春双歧杆菌和长双歧杆菌的两株菌鉴定结果均为动物双歧杆菌

Consider to make clear in recent years, second acid is calcic - Bowman does not move bacillus clinical bacterium of single born of the same parents of the detached rate that go up and verdigris holiday comparatives, have the tendency that year after year rises, the fundamental condition pathogenic bacteria that already became the infection inside the courtyard [1] , have to a variety of antibacterial content be able to bear or endure higher property of a medicine [1, 2] , for this, we are monitored and statistic analysed my courtyard to came 1998 2004 7 years a second acid is calcic - the clinical infection condition that Bowman does not change bacillus, to 14 kinds of antibacterial content be able to bear or endure property of a medicine and be able to bear or endure medical tendency, sum up a report to be as follows now. 1 material and method all data all come from 1.1 basic materials to came on January 1, 1998 my courtyard outpatient service was mixed on December 31, 2004 the specimen sending check of inpatient, via clinical microbiology the lab is fostered and classics appraisal is second acerbity calcium - Bowman does not move bacillus to affect case of illness, with clinical and commonly used antibacterial at the same time content makes the kill that medical quick detects. 1.2 bacteria appraisal is mixed medical quick experiment uses live thing VITEK32 of Mei Li dust is full automatic the bacterial appraisal of bacterial analysis system and its form a complete set gets stuck and medical quick card undertakes detecting, 1998~2000 year use GNI appraisal gets stuck, 2001~2004 uses GNI+ card.

近年探究表明,乙酸钙-鲍曼不动杆菌临床上的分离率和铜绿假单胞菌相当,并有逐年上升的趋向,已成为院内感染的重要条件致病菌[1],对多种抗菌药物有较高耐药性[1,2],为此,我们监测并统计分析了我院1998年至2004年七年间乙酸钙-鲍曼不动杆菌的临床感染情况,对14种抗菌药物的耐药性及耐药趋向,现总结报道如下。1材料和方法1.1基本资料所有资料均来自于1998年1月1日至2004年12月31日我院门诊和住院病人的送检标本,经临床微生物学实验室培养并经鉴定为乙酸钙-鲍曼不动杆菌感染病例,同时以临床常用抗菌药物作药敏检测的结果。1.2细菌鉴定和药敏试验采用生物梅里埃VITEK32全自动细菌分析系统及其配套的细菌鉴定卡和药敏卡进行检测,1998~2000年使用GNI鉴定卡,2001~2004使用GNI+卡。

Background: Gas gangrene, also known as crepitant myonecrosis, is well-known to be most commonly caused by Clostridium species. Previous reports have revealed that nonclostridial involvement may be more prevalent.

背景: 气性坏疽,也称为产气性肌肉坏死,一般认为由梭状杆菌感染造成,但文献指出非梭状杆菌的感染更为大宗。

Difficile is not as strong as that between the infection and antibiotics.

然而,他强调,胃酸分泌抑制剂与难治梭胞杆菌之间的关系并不像感染与抗生素之间那样紧密;必须要重视的是,感染难治梭胞杆菌的最大风险是使用抗生素,而且住院老年病患的风险最高,我们必须了解难治梭胞杆菌引起的感染越来越常见,且越来越严重,因为新的抗药性菌种已经被发现,因为感染这些高抗药性细菌的病患对治疗反应不佳,这样的课题已经受到重视。

The study of morphological, physiological and biochemical characteristics showed that the strain 32-11-2-2 was a slight halophilic strain; it had the morphological characteristics of a continent Bocillus and could produce amylase, fluidify glutin; and it also showed a positive reaction in the litmus milk test. The 16S rDNA analysis showed the strain 32-11-2-2 had a 98% homology with Bacillus subtilis.

结果:分离得到421株细菌,包括活性菌株54株,其中活性菌株32-11-2-2是一株弱嗜盐菌,具有陆地芽孢杆菌的形态特点,能产生胞外淀粉酶、过氧化氢酶,液化明胶、石蕊牛奶反应阳性,16S rDNA序列分析该菌株与枯草芽孢杆菌同源性达到98%以上。

This strain was identified to be a new subspecies of Gluconacetobacter intermedius in the species of Gluconacetobacter.

初步鉴定该菌为葡糖醋杆菌属(Gluconacetobactersp。)一个新菌种或者中间葡糖醋杆菌的一个亚种。

The fermentation, which can require 10 to 12 days, involves the development of Leuconostoc mesenteroides, Enterococcus faecalis, pediococcus cerevisiae, Lactobacillus brevis, and L.

发酵要求10~12天,包括肠膜样明串珠菌,粪链球菌,啤酒片球菌,短乳杆菌和植物乳杆菌的生长

①The collected clinical Acinetobacter baumannii isolates were more seriously resistable to Ciprofloxacin than to Imipenem;②The antibiotic resistance of clinical Acinetobacter baumannii isolates may be associated with the decline of CarO protein expression;③There is some relationship between CarO protein and ciprofloxacin-resistant Acinetobacter baumannii strains,it proves that CarO protein supports bacteria on multi-drug resistance;④CarO protein may be related to cell signalling;⑤The difference in CarO protein functional sites and structure between the sensitive strains and the resistant strains may be related to bacterial resistance.

①收集的临床鲍曼不动杆菌对环丙沙星的耐药情况较亚胺培南严重;②CarO蛋白的表达下调可能与临床鲍曼不动杆菌的耐药性相关;③CarO蛋白与鲍曼不动杆菌耐环丙沙星有一定相关性,佐证了CarO蛋白可能与细菌多重耐药有关;④CarO蛋白可能与细胞信号传导有关;⑤敏感菌株与耐药菌株的CarO蛋白功能位点和内部结构有差异,可能与细菌耐药有关。

"The association between AF and H. pylori was very strong in patients with persistent AF," the authors write."In addition, we report high concentrations of CRP, which confirm the presence of systemic inflammation in patients with AF, which leads us to hypothesise that H pylori infection may be the substrate of this systemic inflammation manifesting in AF.??More data will be necessary from controlled studies to further identify how H. pylori can influence the pathogenesis of AF."

持续性心室纤维颤动患者的心室纤维颤动与幽门螺旋杆菌之间的关系非常强烈;Montenero表示,我们另外也提报了高浓度的C反应蛋白,这对心室纤维颤动患者的全身发炎现象作了证实;这个现象使得我们作出一项假设,亦即,心室纤维颤动患者之所以会全身发炎,是因为幽门螺旋杆菌的关系;至於幽门螺旋杆菌如何对心室纤维颤动发病原因作影响,我们需要更多的资料才能证实。

The isolated cells are able to orient and migrate to the magnet in artificial magnetic field but could not orient swimming only under the geomagnetic field. Magnetosomes produced by Acidithiobacillus ferrooxidans were range from 40nm to 90 nm according to the results of TEM. Energy dispersive X-ray analysis indicated that extracted magnetic particles consisted of oxygen and iron. The results show that some Acidithiobacillus ferrooxidans cells have weak magnetotaxis and they could be able to be separated by solid-plate magnetophoresis method from others. With the development of this new isolation method, it is possible that we could do deeper research to generate a comprehensive description of the mechanism that how Acidithiobacillus ferrooxidans synthesize the magnetic particles.

实验结果表明,氧化亚铁硫杆菌具有较弱的趋磁性,在人工磁场下会进行趋磁运动,但仅在地磁场作用下不能定向运动,利用固体平板磁泳筛选纯化含有磁性颗粒的氧化亚铁硫杆菌的方法是切实可行的,磁泳分离技术的进一步完善和改进为传统的微生物菌种分离提供了新的途径,为研究纯氧化亚铁硫杆菌菌株胞内磁性颗粒的形成条件及机理提供了前提条件,也为今后从浸矿细菌中分离筛选更多的含有磁性颗粒的菌株打下基础。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。