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The results showed: the Berberis heteropoda pigment could effectively inhibit the growth of theE scherichiac oli, the calyx pigment ofP hysalis alkekengi coulde ffectively inhibit the growth of the Bacterium proteus, the concentrated liquid of Rhodiola sachalinensis could effectively inhibit the growth of the Bac.

结果发现:小檗色素对大肠杆菌抑菌作用最强,酸浆花萼色素对变形杆菌抑菌作用最强,红景天浓缩液对枯草芽孢杆菌抑菌作用最强,小茴香浓缩液对四种待试菌无抑菌作用;小檗色素对四种待试菌的最低抑菌浓度和最低杀菌浓度分别为:枯草芽孢杆菌MIC2。

The results showed the population of endophytic bacteria from oilseed rape was very abundant.These bacteria species is the first reported in oilseed rape, and Mycoplana bullata、Acinetobacter calcoaceticus、Pantoea dispersa、Pantoea ananas、Enterobacter cowanii、Pseudomonas fulva、Pseudomonas parafulva are new record species of endophytic bactria..

通过16S rDNA 序列系统发育分析对PCR-RFLP 划分的主要类群进行了鉴定,共鉴定出内生细菌16 种,分别是,分别为:蜡状芽孢杆菌、泡状枝动菌、醋酸钙不动杆菌、鲍氏不动杆菌A。

Results:The level of total anaerobic population and total aerobic population of excrement specimens of three kinds of IBS sufferers were different significantly, particularly the level of bifidobacterium and cillobacterium(P.01);the level of bacteroid population was higher significantly in diarrhea-predominant and mixed type of IBS sufferers (P.01);the level of enterococcus population was higher significantly in diarrhea-predominant IBS sufferers(P.05);among the aerobes, the number of Escherichia coli and Staphylococcus aureus colonies were increased by different level(P.01or0.05) in the IBS suffers, in particular, the number of Escherichia coli colonies in diarrhea-predominant IBS sufferers increased most significantly.

结果:三种类型的IBS患者粪便中总厌氧菌群和需氧菌群数目与健康人差异明显,特别是双歧杆菌属和乳酸杆菌属明显减少(P.01;拟杆菌菌落数在腹泻型和混合型IBS患者中明显增加(P.01);肠球菌菌落数在腹泻型IBS患者中明显增加(P.05);需氧菌中患者组大肠杆菌和金黄色葡萄球菌菌落数均不同程度增加(P.01或0.05),大肠杆菌菌落数在腹泻型IBS患者中增加最明显。

Results: The proportion of Gram-negative bacilli rose obviously, so did the opportunistic pathogen's and Yeasts detected rate. Most drug sensitive tests showed the drug-fast rate turned to be high. Escherichia coli, Salmonella and Citrobacter resistant and so on to fluoroquinolones was 57%~97%. These bacteria to imipenem showed excellent activity. Meticillin-resistant-staphylococcus resistant to ciprofloxacin, norfloxacin, clindamycin, tetracycline, erythromycin, cefazolin and ampicillin were 40.9%, 42.4%, 63.6%, 72.2%, 85.9%, 100.0% and 100.0%, respectively Streptococcus and Enterococcus resistant to major β-lactams-penicillin, aminoglycosides, macrolides, tetracyclines and sulfonamides were between 36.0% to 100.0%.

结果:革兰阴性杆菌比例显著升高,条件致病菌和酵母样真菌检出机会显著增多;药敏结果显示多数临床细菌对常用抗生素的耐药趋势在不断上升;大肠埃希菌、沙门菌和枸橼酸杆菌等革兰阴性杆菌对喹诺酮类药物的耐药率达57%~97%,亚胺培南则对阴性杆菌有较高的敏感覆盖率;耐甲氧西林葡萄球菌对环丙沙星、诺氟沙星、氯洁霉素、四环素、红霉素、头孢唑啉和氨苄西林的耐药率分别为40.9%、42.4%、63.6%、70.2%、85.9%、100.0%和100.0%;链球菌和肠球菌对多数β内酰胺类青霉素、氨基糖苷类、大环内酯类、四环素类以及磺胺类药物均呈高度耐药,耐药率在36%~100%。

In the air common pathogenic bacteria, including Streptococcus, Staphylococcus aureus, meningococcus, Mycobacterium tuberculosis, pertussis bacteria, Legionella bacteria, Bacillus anthracis, diphtheria bacteria, Mycoplasma pneumoniae, Rickettsia, and so on.

空气中常见的致病菌包括溶血性链球菌、金黄色葡萄球菌、脑膜炎双球菌、结核杆菌、百日咳杆菌、军团菌、炭疽杆菌、白喉杆菌、肺炎支原体、立克次氏体等。

Pasteurization, which is often used for milk or waste milk, can be used to reduce bacteria levels and has been shown to be very effective at killing a variety of pathogenic bacteria including Salmonella, E. coli, Mycobacterium avium subspecies paratuberculosis, Mycobacterium californicum, Mycobacterium bovis, and Listeria monocytogenes.

巴氏灭菌常使用于牛奶或牛奶废料,它可以用来降低细菌水平,并已被证明能有效的杀死多种致病细菌,其中包括沙门氏菌、大肠杆菌、副结核分枝杆菌亚种、californicum分枝杆菌、牛分枝杆菌和李斯特菌。

Respectively use chelex 100 resinate,boiling extraction,proteinase K digest/isopropanol precipitation,proteinase K digest/phenol/chloroformextra ction,alkaline lysis,guanidine thiocyanate lysis,guanidine hydrochloride lysis,spin column method to extract Mycobacterium tuberculosis DNA for multiplepolymerase chain eraction.

选择3份含结核分枝杆菌分别为4.325×103、6.857×104、5.356×105 Copies/ml的痰液,分别用Chelex 100树脂法、煮沸法、蛋白酶K消化异丙醇沉淀法、蛋白酶K消化酚/氯仿抽提法、碱裂解法、异硫氰酸胍裂解法、盐酸胍裂解法、离心柱法抽提结核分枝杆菌DNA作多重聚合酶链反应,显色法芯片检测结核分枝杆菌RFP和INH耐药基因。

And Citrobacter sp., respectively. Both of them were γ-proteobacteria. 3 IPB strains closely matched to Bacillus megaterium, Terrabacter tumescen and Janthinobacteriwn lividum. respectively, and 6 OPB strains were Azospirillum sp., Enterobacter aerogenes. Blastobacter aggregates, Staphylococcus epidermidis, Acinetobacter haemolyticus and Cryptococcus sp., respectively.

对3株有代表性的无机磷分解菌和6株占优势的有机磷分解菌的鉴定显示,3株无机磷分解菌与巨大芽孢杆菌、大地杆菌和Janthinobacterium lividum最相近,而6株有机磷分解菌分别为固氮螺菌,产气肠杆菌,Blastobacter aggregatus,表皮葡萄球菌,溶血不动杆菌和念珠菌。

Unsurprisely,These three microorganisms decreased as the storage time increases.84 strains lactobacillus were isolated during the detection period in the pickled gingers stored in low temperature.By physiology and biochemistry methods,32 strains L.plantarums,6 strains L.brevis,2 strains Lacto...

低温保存泡姜在整个检测期内,共分离到84株典型乳酸菌,经生理生化实验鉴定出植物乳杆菌32株、弯曲乳杆菌7株、短乳杆菌6株、肠膜明串珠菌属5株、乳球菌属2株、乳杆菌属32株;酵母菌分离鉴定出3个属,主要为酵母属以及少量的假丝酵母属和汉逊氏酵母属;只在保存的前2d检测到个别的青霉属和毛霉属;在整个检测期内,没有分离到大肠杆菌。

The results of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L,there existed some stable hydrophobic regions,which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid,in the partially folded intermediate of Bacillus amyloliquefaciensα-amylase;with the denaturation concentration increasing,the stable hydrophobic regions disappered.the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed that using acrylamide as quenchers,with the protein denaturation extent increasing,the number of Trp that can be quenched increased untill all the Trp residues were quenched;Using potassium iodide as quenchers,with the maximum number(8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciensα-amylase molecule could be quenched by potassium iodide;with the denaturation concentration increasing,the number of Trp that can be quenched decreased to 5.the results of their protein electrophoreses and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciensα-amylase formed during the whole unfolding/refolding procedure of Bacillus amyloliquefaciensα-amylase induced by guanidine hydrochloride or urea.

ANS外源荧光探针结果表明:盐酸胍诱导的芽孢杆菌α-淀粉酶分子去折叠过程中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域;而随着芽孢杆菌α-淀粉酶分子在盐酸胍溶液中变性程度的加深,这一疏水区域逐步被瓦解。丙烯酰胺和碘化钾猝灭结果表明:在盐酸胍溶液中,随着芽孢杆菌α-淀粉酶分子变性程度的进一步加深,其分子内能够被丙烯酰胺接近的色氨酸残基逐渐增多,直至全部被猝灭。但位于芽孢杆菌α-淀粉酶分子表面的能够被碘化钾猝灭的色氨酸残基,在中间态芽孢杆菌α-淀粉酶分子中数目达到最大的8个,而随着其分子变性程度的进一步加深,反而减少至5个。

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