杆菌的

There are several distinct pathogens: Brucella abortus, B. canis, B. melitensis, B. suis, whose identifications correlate with their reservoir s, respectively, cattle, dogs, goats and sheep, and pigs.

有若干不同的病原体：流产布鲁氏杆菌、犬布鲁氏杆菌、山羊布鲁氏杆菌、猪布鲁氏杆菌等，其识别与对应的贮藏宿主有关，即分别为牛、犬、山羊和绵羊、猪。

There are several distinct pathogens: Brucella abortus, B. canis, B. melitensis, B. suis, whose identifications correlate with their reservoirs, respectively, cattle , dogs, goats and sheep, and pigs.

有若干不同的病原体：流产布鲁氏杆菌、犬布鲁氏杆菌、山羊布鲁氏杆菌、猪布鲁氏杆菌等，其识别与对应的贮藏宿主有关，即分别为牛、犬、山羊和绵羊、猪。

There are several distinct pathogen s: Brucella abortus, B. canis, B. melitensis, B. suis, whose identifications correlate with their reservoirs, respectively, cattle, dogs, goats and sheep, and pigs.

有若干不同的病原体：流产布鲁氏杆菌、犬布鲁氏杆菌、山羊布鲁氏杆菌、猪布鲁氏杆菌等，其识别与对应的贮藏宿主有关，即分别为牛、犬、山羊和绵羊、猪。

There are several distinct pathogens: Brucella abortus, B. canis, B. melitensis, B. suis, whose identifications correlate with their reservoirs, respectively, cattle, dogs, goat s and sheep, and pigs.

有若干不同的病原体：流产布鲁氏杆菌、犬布鲁氏杆菌、山羊布鲁氏杆菌、猪布鲁氏杆菌等，其识别与对应的贮藏宿主有关，即分别为牛、犬、山羊和绵羊、猪。

Besivance is indicated for the treatment of bacterial conjunctivitis in patients aged ≥1 year whose infection is caused by susceptible isolates of the following bacteria: CDC coryneform group G, Corynebacterium pseudodiphtheriticum, Corynebacterium striatum, Haemophilus influenzae, Moraxella lacunata, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus hominis, Staphylococcus lugdunensis, Streptococcus mitis group, Streptococcus oralis, Streptococcus pneumoniae, and Streptococcus salivarius.

Besivance适用于治疗年龄≥1 岁、由下列细菌的致病分离物所致的细菌性结膜炎患者：CDC棒状杆菌G群，假白喉棒杆菌，纹带棒杆菌，流感嗜血杆菌，腔隙莫拉菌，金黄色葡萄球菌，表皮葡萄球菌，人葡萄球菌，路邓葡萄球菌，缓症链球菌群，口腔链球菌，肺炎链球菌以及唾液链球菌。

The low temperature biofilm was composed of physchrotrophs, Through the experiments of isolation and identification, 8 strains of physchrotrophs were obtained from biofilm, they are Comamonas、Flavobacterium、Arthrobacter、Bacillus、Micrococcus、Pseudomonas、Achromobacter and Vibrio.

低温生物膜的活性主要来自其上面的低温微生物，分离鉴定试验结果表明，组成低温生物膜的微生物主要有8种，发现它们分别属于丛毛单胞菌属、黄杆菌属、节杆菌属、芽孢杆菌属、微球菌属、假单胞杆菌属、无色菌属和弧菌属。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

Fluorescent instruments.the colonies of the specimens yielding growth were identified by the vitek32 instruments and kb method was used for the drug sensitivity tests.results three hundred and thirtyone bacteria strains were isolated from a total of 3104 blood culture specimens,with a positive rate of 10.7%.of the identified bacteria,g+ cocci accounted for 50.3%,g-bacilli accounted for 44.7%,fungi accounted for 3.0%,and anaerobian accounted for 1.1%.the susceptibility rates of g-bacilli to imipenem,amikacin and cefoperazone/sulbactam were high.the susceptibility rate of g+ cocci to vancomycin and imipenem were also high.conclusion g+ cocci prevail over g-bacilli in the blood specimens.the staphylococci are the chief bacteria in the childrens blood specimens.both g+ cocci and g-bacilli are sensitive to imipenem.

结果 在3 104份血液培养标本中分离出病原菌331株，阳性检出率为10.7%。病原菌以革兰阳性需氧球菌居首位（50.3%），革兰阴性需氧杆菌次之（44.7%），真菌占3.0%，厌氧菌占1.1%。血液培养中的g+球菌对万古霉素和亚胺培南较为敏感，g-杆菌对亚胺培南、舒普深、丁胺卡那较为敏感。结论血液培养病原菌以g+球菌为主，g-杆菌次之；儿童血液培养病原菌以葡萄球菌属为主；亚胺培南对g+球菌和g-杆菌均具有较高的敏感率。

Methods From Feb 2006 to Jun 2006,188 hospitalized children in Shenzhen children s hospital, were collected deep tracheal aspirate at the time of hospitalization. The respiratory tract secretions were immediately sent for bacterial culture with 3 kinds of medium:ordinary medium, Hemophilus influenzae selective medium, Streptococcus penumoniae selective medium. Then we extracted the total nucleic acids from secretions, and detected Mycoplasma pneumoniae by single fluorescent quantitation PCR. Simultaneously, 14 respiratory tract pathogenic bacterium and Mycoplasma pneumoniae were detected by Target Enriched multiplex PCR. Amplification products were identified by the Luminex100 suspension array.

确诊为社区获得性肺炎的患儿188例，在入院当天采集深部呼吸道吸引物，用普通培养基和肺炎链球菌、流感嗜血杆菌选择性培养基进行细菌培养，然后提取深部呼吸道吸引物中病原体的DNA，采用荧光定量单PCR的方法检测肺炎支原体，并对同一标本采用靶序列富集多重PCR技术同时扩增肺炎链球菌、流感嗜血杆菌、金黄色葡萄球菌、肺炎克雷伯菌、大肠杆菌、嗜肺军团菌、铜绿假单胞菌、鲍曼氏不动杆菌、脑膜炎奈瑟氏菌、阴沟肠杆菌、奇异变形杆菌、化脓链球菌、粪肠球菌及屎肠球菌14种呼吸道病原菌和肺炎支原体的靶基因，扩增产物用Luminex100多功能悬浮点阵仪检测。

The results of its intrinsic fluorescence spectroscopy and fluorescence phase diagram showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed a partially folded intermediate of Bacillus amyloliquefaciens a-amylase during its unfolding procedure, which followed a three-state model; the result of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed some stable hydrophobic regions, which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid, in the partially folded intermediate of Bacillus amyloliquefaciens a-amylase; and the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed the distribution of Trp residues in Bacillus amyloliquefaciens a-amylase in different denaturation solution, with the maximum number (8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciens a-amylase molecule could be quenched by potassium iodide; and the results of their protein electrophoresis and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciens a-amylase formed during the whole unfolding procedure of Bacillus amyloliquefaciens a-amylase induced by guanidine hydrochloride.

内源荧光光谱和荧光相图结果表明，当变性液中盐酸胍浓度约为1.0 mol/L时，芽孢杆菌a-淀粉酶的去折叠过程中出现一个部分折叠中间体，其去折叠过程符合&三态模型&；荧光探针结果表明，在溶液中盐酸胍浓度约为1.0 mol/L时，中间态芽孢杆菌a-淀粉酶分子中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域；荧光猝灭研究给出了不同程度变性的淀粉液化芽孢杆菌a-淀粉酶中的Trp的分布情况，结果表明中间态芽孢杆菌a-淀粉酶分子中能够被碘化钾猝灭的位于分子表面的色氨酸残基数目达到最大的8个；蛋白电泳和体积排阻色谱结果表明，在盐酸胍诱导的芽孢杆菌a-淀粉酶分子的整个去折叠过程中，不会以共价键或非共价键形式形成芽孢杆菌a-淀粉酶分子之间的集聚体或集聚体沉淀。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。