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Results Causing the pathogen of the infection regard the Green's surname feminine gender rod bacteria as principle,occupying 90.2%,the escherichia coli is the most familiar to occupy 65% the next in order for transform the rod bactenia,viceescherichia coli,sewerage intestine rod bacteria,etc.of aerobacter aerogenes.

结果:引起感染的病原体以革兰阴性杆菌为主,占90.2%,大肠埃希菌最常见占65%,其次为变形杆菌、副大肠杆菌、阴沟肠杆菌、产气杆菌等。

From 1983 since, american researcher already produced toxin of an anthrax bacili, A flesh successfully with contemporary biology technology family name of congratulate of bacillus, choleraic vibrio, annals is dysenteric the gene of bacillus and toxin of buccal larynx bacili.

从1983年以来,美国探究人员已用现代生物技术成功生产出炭疽杆菌、A 型肉毒杆菌、霍乱弧菌、志贺氏痢疾杆菌和口喉杆菌毒素的基因。

This paper studies the effects of Jerusalem artichoke on the growth of Bifidobacterium in skim milk in order to provide theoritical foundation for the utilization of Jerusalem artichoke resource and development of bifidus yogurt, using Jerusalem artichoke powder as experimental material and B.

以菊芋粉为材料,以长双歧杆菌和两歧双歧杆菌为实验菌株,研究菊芋在脱脂乳中对双歧杆菌生长的影响,以期为进一步开发菊芋资源和研制双歧杆菌酸奶提供理论依据。

Results:The results showed that the extraction of Milpa Alta can obviously inhibit the growth of the Staphylococcus aureaus、 Hemolytic B Streptococcus、 Proteus species、Bacillus subtilis、Bacillus cereus、Staph.Epidermidis、Escherrichia coli andP.aeruginosa.

结果:"米邦塔"仙人掌汁对金黄色葡萄球菌、大肠杆菌、绿脓杆菌、乙型溶血性链球菌、变形杆菌、枯草牙孢杆菌、蜡状芽孢杆菌、表皮葡萄球菌均有明显的抑制作用。

Responsible for constructional planning of clinical application research, and its implementation, along with the examination studies on intestinal tract micro ecology and hematological attributes of AIDS patients, which including the quantitative tests of Bifidobacterium, Lactobacillus, Bacteroides, Enterobacter, Escherichia coli and cl.perfringen in feces.

负责临床应用总体设计和临床应用研究,负责艾滋病人血液指标及肠道微生态学检测研究,其中包括:粪便中双歧杆菌、乳杆菌、拟杆菌、肠杆菌、肠球菌、产气荚膜梭菌数量的检测。

After avilamycin 8mg/kg was fed to Cyprinus carpio, the quantity of the intestinal aerobic and the facultative anaerobic bacteria, the percent of Enterobacteriaceae, and the quantity of Lactobacillus decreased significantly on the 14~ d, at the same time , the percent of Flavobacterium increased evidently, the quantity and percent of Bacteriodes decreased evidently during the first 28 d of the experiment.3 After Cyprinus carpio was fed with narasin, the percent of Corynebacterium increased.

投喂阿维拉霉素8mg/kg在试验第14d使鲤肠道好氧及兼性厌氧菌总数、肠杆菌科所占百分比、乳杆菌属数量显著降低,黄杆菌属所占百分比显著升高;在试验的前28d使拟杆菌属数量及其百分比显著下降。

TBA analysis showed 0.01% of TP had evident antioxidation on lipids from fish meat, and TBA increased with the TP concentration accretion presenting an unlinear relation. The effects of antioxidation of 0.03% and 0.07% of TP were close, thus, the best choice is 0.03% in practical application.5. 93 bacteria strains were isolated and categorized into several spoilage bacteria classes: Aeromonas, Vibrio, Pseudomonas, Enterobacteriaceae, Flavobacterium, Acinetobacter, Photobacteriaceae, Brevibacterium, and Micrococcus. In the early stage of storage (1~3 days), Gram-positive bacteria were the major strains and accounted for 70%, most of them belonged to Brevibacterium and Micrococcus.

TBA试验结果显示,添加00.1%以上茶多酚对草鱼脂肪都有明显的抗氧化作用,随浓度增加TBA值增加,但不呈线性关系,0.03%~0.07%抗氧化效果比较接近,从经济、有效的角度考虑在实际应用中可采用0.03%的茶多酚。5、从草鱼中分离出93株菌株,鉴定出以下几类主要腐败细菌:气单胞菌属、弧菌属、假单胞菌属、肠道杆菌科、黄杆菌属、邻单胞菌属、发光杆菌属、产碱杆菌属、短杆菌属、微球菌属、不动杆菌属、无色菌属。

The biotransformations of isoeugenol to vanillin with various strains available in our lab were tested. It was found that several baterica including Bacillus subtilus 7658, Alcaligenes AS1.7617, Brevibacterium flavum 1.7a and Brevibacterium flavum AS1.013 could convert isoeugenol to vanillin.

对实验室所保藏的细菌、放线菌、真菌进行初步筛选发现细菌中枯草芽孢杆菌7658、粪产碱杆菌AS1.7617、黄色短杆菌1.7a、黄色短杆菌AS1.013能产生少量香草醛,最高浓度为0.31 g/L;放线菌中诺卡氏菌Nocardia sp。

The fusion DNA fragments of ag85b-mpb64 and ag85b-mpb64-esat-6 were obtained by PCR andSOE technique. Various DNA vaccines were constructed with the pcDNA3.1: fusion of two genes, and of three genes, bivalent combinations and trivalent combinations(pCA+pCM+pCE6). BALB/c mice were vaccinated with this DNA vaccines.The mice injected withBCG were positive control and the mice injected with pCDNA3.1 and PBS were negative control.The mice were immunized 3 times with 2-wk intervals. The animals in group BCG were only inoculatedsubcutaneously with 1×10~6 CFU BCG at initial vaccination. The serum IgG titers and IgG isotype weredetermined using iELISA coated with M. bovis PPD and rMAE protein expressed and depurated inprokaryotic expression system every week.

同样,利用PCR和SOE技术,获得牛分枝杆菌mpb64-ag85b和mpb64-ag85b-esat-6融合基因,以pCDNA3.1为载体构建了牛分枝杆菌多价组合和多基因融合DNA疫苗:二基因融合(pCDNA3.1-MPB64-Ag85B,简称pCMA)和三基因融合(pCDNA3.1-MPB64-Ag85B-ESAT-6,简称pCMAE)DNA疫苗;二价组合和三价组合(pCA+pCM+pCE6)DNA疫苗,免疫BALB/c小鼠,以牛分枝杆菌BCG免疫组为阳性对照,以pCDNA3.1及PBS免疫组为阴性对照,共免疫3次,每次间隔2周,BCG组仅初免时皮下免疫1次。1免后每周,以原核表达纯化的重组MPB64-Ag85B-ESAT-6蛋白和牛分枝杆菌PPD为包被抗原,以间接ELISA方法检测血清IgG水平及lgG亚类。

Objective To survey the prevalence of Helicobacter pylori in gastrointestinal out-patient department in Han group in Hainan. Methods There 2 395 patients from ten counties or cities in Han group in Hainan at gastrointestinal out-patient department of the hospital were detected by rapid urease test of H.pylori and gastroscope. The posititives of rapid urease test of H.pylori were determined with 14C-urea breath test (14C-UBT).Two positive tests were confirmed to be infected with H.pylori. Biopsy was performed on 1 004 patients positive for H.pylori. 113 healthy people were enrolled in control group.

目的 调查海南汉区在消化内科就诊患者中幽门螺杆菌(Helicobacter pylori,H.pylori)感染患病情况,以及幽门螺杆菌感染与胃黏膜病理的关系方法选择海南汉区10 县市2 395例具有上消化道症状就诊患者,经胃镜检查并取胃窦黏膜做H.pylori快速尿素酶试验,阳性患者做14C呼气试验,两者阳性确定幽门螺杆菌感染。1 004例幽门螺杆菌阳性患者取胃窦及病变部位胃黏膜做病理组织检查。

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