杆菌的

In this paper, a new product of Ce/ TiO2 compound antibacterial agent has been made, comparing with naked nano－TiO2, it has better antimicrobial activity against E.coli ,S.aureus and scorch gemmule bacillus in the solar light, increasing the quantity of Ce4+, exhibiting stronger antibacterial property.

制备出稀土铈掺杂锐钛型纳米TiO2复合抗菌功能材料，该抗菌材料与不掺杂的纳米TiO2相比，在可见光下对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌的混合菌具有较好的抑菌效果，随着Ce4+的摩尔浓度增加，制备的试样的抗菌能力越强。

Among them strain MZ1 had been confirmed a lysogenic strain by indicator strain ZK1 after producing phage plaques induced by Mitomycin C.Two templates of overnight cultivation and its DNA from strain MZ1 were then amplified in the same time with five pairs of primers designed from the same phage,and the same products were obtained,suggesting that strain MZ1 was also confirmed a lysogenic strain by PCR method.

MZ1菌株的过夜培养液与指示菌ZK1(血清型H25)混匀并在双层琼脂上培养12 h后出现了滴度为2×107 pfu的噬菌斑，从而证明生产菌株MZ1确为溶原菌；又以GIL01同源性较高的其它四个基因设计引物，分别以MZ1的培养物及其溶原性噬菌体基因组为模板进行PCR扩增，结果两者得到几乎一致的带型，进一步证明了生产菌株MZ1为溶原菌，说明利用PCR法检测鉴定苏云金杆菌的溶原性是可行的。

The results showed that: the explants of strawberry leaves were rather sensitive to kanamycin, and the corresponding concentration of 20 mg/L could substantially inhibit the growth of non-transformed tisse and be used to screen the leaves of strawberry after transformation. Timentin at the concentration of 300 mg/L could be used to eliminate Agrobacterium tumefaciens and had minor impact on the growth of the explants. The preculture time of 2-4 days was beneficial to improve transformation frequency. Co-culture for 2-3 days not only had higher gus transient expression but also avoid the over-growth of agrobacterium. The bacterial concentration of OD(subscript 600nm) 0.4 was the best inoculum density and 10 minutes was the best inoculation time.

结果表明：草毒叶片外植体对卡那霉素较为敏感，适宜的筛选浓度为20mg/L，可明显抑制非转化组织的生长；300mg/L的羧吩青霉素可有效抑菌，对外植体生长影响也较小；预培养2-4d有利于转化；共培养2-3d有利于提高转化频率并避免了农杆菌的过度生长；OD(下标 600nm)值为0.4的菌液侵染10min效果最佳。

The preculture time of 2-4 days was beneficial to improve transformation frequency.Co-culture for 2-3 days not only had higher gus transient expression but also avoid the over-growth of agrobacterium.The bacterial concentration of OD_(600nm) 0.4 was the best inoculum density and 10 minutes was the best inoculation time.

结果表明：草莓叶片外植体对卡那霉素较为敏感，适宜的筛选浓度为20mg/L，可明显抑制非转化组织的生长；300mg/L的羧吩青霉素可有效抑菌，对外植体生长影响也较小；预培养2-4d有利于转化；共培养2-3d有利于提高转化频率并避免了农杆菌的过度生长；OD600nm值为0.4的菌液侵染10min效果最佳。

At last, the effects of JA on the bile salt resistance of Bifidobacteria were studied.The test proved that:Deoxycholic acid Na-Salt had intensely toxical action on Blm and Bbm;Adding glucose and fructose in media could decrease the lexical action on Bbm.but inulinand JAP had not apparent effect.

初步研究了菊芋对双歧杆菌的胆盐耐受性的影响，实验表明，脱氧胆酸钠盐对Blm和Bbm有一定毒性作用；在培养基中补充一定量的葡萄糖和果糖可以改善Bbm的胆盐耐受性，而菊糖和菊芋粉对提高Blm和Bbm的胆盐耐受性没有明显作用。

OBJECTIVE:Proton pump inhibitor is a new effective drugs for the treatment of peptic ulcer,it alternatively inhibits H+/K+-ATP coenzyme and secreetion of hydrochloric acid , sterilizes helicobacter pylori.This paper summarizes the aspect of clinical usage and evaluate their clinical efficacy and safety.

目的：质子泵抑制剂的问市开辟了药物对抗消化性溃疡的作用途径，其选择性地抑制体内H+/K+-ATP酶活性和酸的分泌，同时对胃幽门螺杆菌的感染也具良好的杀灭作用，本文对其进展和临床应用进一步评价。

To explore the effect of coculturing actinomycetes with Bacillus subtilis on the production of bioactive secondary metabolites, we studied the difference between fermentation products of monocultures and the corresponding cocultures of 22 actinomycetes by antimicrobial assay and HPLC-PDA analysis.

为探讨共培养对放线菌产生活性次生代谢产物的影响，结合抗菌活性测定及HPLC-PDA分析，研究了22株放线菌的单培养及其与枯草芽孢杆菌的共培养发酵代谢产物的差异，并选取抗菌活性较强的链霉菌FXJ2.014进一步研究其代谢产物。

To explore the effect of coculturing actinomycetes with Bacillus subtilis on the production of bioactive secondary metabolites, we studied the difference between fermentation products of monocultures and the corresponding cocultures of 22 actinomycetes by antimicrobial assay and HPLC-PDA analysis.

摘 要：为探讨共培养对放线菌产生活性次生代谢产物的影响，结合抗菌活性测定及HPLC-PDA分析，研究了22株放线菌的单培养及其与枯草芽孢杆菌的共培养发酵代谢产物的差异，并选取抗菌活性较强的链霉菌FXJ2.014进一步研究其代谢产物。

Methods: The minimum inhibitory concentrationof Xipayiguyiy on P.gingivalis,P.intermedia and F.nucleatum was observed by agar dilution in vitro.Also,the effects of Xipayiguyinye on oral malodour challenged by cysteine was investigated on 10 volunteers in vivo.Results: It showed in this study the MIC of Xipayiguyiy for Pg and Fn was 1mg/mL,and for Pi was 0.5mg/mL.It was also found the Xipayiguyinye achieved significant effects on oral malodour challenged by cysteine.

采用琼脂稀释法，测定西帕依固龈液对牙龈卟啉单胞菌（Pg、中间普雷沃菌、具核梭杆菌的最低抑菌浓度；对10例志愿者采用自身前后对照试验，观察四种干预措施（无干预、清水、西帕依固龈液、口泰）对半胱氨酸激发性口臭的最大值水平、各时间点下降百分比及口气值的下降曲线的影响，评价西帕依固龈液防治口臭的效果。

The apxⅢA gene of Actinobacillus pleuropneumonie was amplified by PCR. The amplified DNA fragment 3 466bp was cloned into pMD18-T. After R.E. analysis and sequencing, the apxⅢA gene in pMD18-T was ligated into pBluescripⅡSK, the recombinant expression plasmid pET-28b/apxⅢA was constructed and analysed with R.E., the protein of apxⅢA gene expressed in E.coli BL21 was detected by Western blotting.

因胸膜肺炎放线杆菌的致病性主要是由毒素决定的，故参照猪胸膜肺炎放线杆血清2型菌株的序列(GenBankL1 2 1 4 5)设计了一对特异性引物，用PCR的方法扩增apxⅢA基因并得到了长 3 466bp的片段，然后将其克隆到pMD 1 8T中，经酶切鉴定和序列分析表明克隆是成功的；再将apxⅢA插入到原核表达载体pET 2 8b后，转化BL2 1 (DE3)，在IPTG诱导下获得高效表达，经Westernblotting检测证实表达产物有活性。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。