杆菌的

Results: The MBCs of ethanol, glutaraldehyde disinfectant, ShiKang disinfectant and quaternary ammonium disinfectant against multidrug-resistant Acinetobacter baumannii were 45%, 10000 mg/L, 6.3~12.5 mg/L and 100 mg/L, and the MBCs were 40%, 5000 mg/L, 3.1~6.3 mg/L and 50 mg/L Conclusion: MBC and MIC could represent the sensitive dose of multidrug-resistant Acmetoborter baumannu to disinfectants, and eight strains of multidrug-resistant Acinetobacter baumannii have certain resistance to some common disinfectants.

结果：乙醇、戊二醛消毒液、施康消毒液、季铵盐消毒剂对多重耐药鲍曼不动杆菌的MBC值分别为45%、10000 mg/L、6.3~12.5 mg/L、100 mg/L；MIC值分别为40%、5000 mg/L、3.1~6.3 mg/L、50 mg/L、50 mg/L。结论：MBC值和MIC值能基本反应多重耐药鲍曼不动杆菌对消毒剂的敏感剂量，8株多重耐药鲍曼不动杆菌对部分消毒剂产生一定程度的耐药性。

Each embryo were inoculated by a dose of 1×104 pure sporozoites was injected with testing Bt crystal proteins before 24 hours. The varying oocyst inhibition rates of embryos brought about by Bt crystal proteins that were given at various doses were used as a criterion for determining the activity of Bt crystal proteins.

每一鸡胚接种1×104个子孢子，于感染前24h注入苏云金杆菌晶体蛋白，以不同苏云金杆菌晶体蛋白对鸡胚卵囊所产生的抑制率的差异，判定苏云金杆菌的活性。

The results demonstrated that 22 of 50 were positive by PCR-ELISA method while only 7/50 and 18/50 by antiacid staining and culture respectively, and no positive result was found in 20 sputum specimens without Mycobacterium tuberculosis by the methods; Conclusion The pCR-ELISA method can objectively detect Mycobacterium tuberculosis from clinical specimens with high specificity and sensitivity.

对50份来自结核病人高度怀疑有结核分枝杆菌的标本检测表明，PCR-ELISA检出22份阳性，比抗酸染色法(7/50)、培养法(13/50)和PCR-电泳法(18/50)检出率高，而20份证实无结核分枝杆菌的标本，几种方法检查均为阴性。结论该法可敏感、特异和客观地检测临床标本中的结核分枝杆菌。

Strains YL001 and YL002 formed a monophyletic clade with strains of X. nematophilus with sequence homology outweighing 99% and the sequence homology to genus Photorhabdus outweighing 94%. The lag, logarithmic, stationary and contabescence phase of the two bacteria were 0-6, 6-18, 18-66 and 66 h respectively; pH of strains YL001 and YL002 reduced to 5.70 and 5.56 at 12 h, respectively, and then rose gradually to 7.74 and 8.07 respectively when culture finished. Glucose content reduced quickly during 0-18 h, and then kept stable. Amido nitrogen content reached the lowest at 12 h and then rose slowly. YL001 exhibited the highest inhibitory effects at 54 h and YL002 exhibited highest inhibitory effects at 42 and 66 h respectively on B. cirerea and B. subtilis. Strains YL001 and YL002 belonged to X.

YL001和YL002菌株与嗜线虫致病杆菌种内菌株形成一个类群，序列同源性大于99%，与发光杆菌属内菌株的序列同源性大于94%。2菌株的延缓期、对数生长期、稳定期和衰亡期分别为0~6，6~18，18~66和66 h；培养12 h后，YL001和YL002菌株发酵液的pH值分别降低至5.70和5.56，此后逐渐上升，至发酵结束时其pH值分别为7.74和8.07；培养0~18 h时2菌株发酵液中还原糖含量迅速降低，此后保持稳定；12 h时氨基氮含量达到最低，此后开始缓慢上升；YL001菌株培养54 h后及YL002菌株培养42和66 h后，其对番茄灰霉病菌和枯草芽孢杆菌的抑制作用最强。

The determination of differential proteins between Mycobacterium tuberculosis and nontuberculosis mycobaeteria may he helpful to differentiation of mycobacteria.

结核分枝杆菌和非结核分枝杆菌差异蛋白的发现，可能有助于分枝杆菌的鉴别。

MBC value was 0.61, 0.304, and 0.00475 μgmL^(-1) respectively. Sterilization rate of ceftiofur suspension was the fastest to Actinobacillus, followed by Streptococcus. PAE of ceftiofur suspension was relatively short to E. coli, which illustrated that it was nonconcentration dependent. PAE was obvious longer to Actinobacillus, which showed that it was concentration dependent, however, it revealed part concentration dependent to streptococcus.

头孢噻呋混悬剂对大肠杆菌、放线杆菌及猪兽疫链球菌的MIC值分别为：0.076、0.304、0.00475μgmL^(-1)；MBC值分别为：0.61、0.304、0.00475μgmL^(-1)；头孢噻呋混悬剂对放线杆菌的杀菌速率最快，对链球菌次之；头孢噻呋混悬剂对大肠杆菌PAE较短，呈现非浓度依赖性，对放线杆菌的PAE较长，呈明显的浓度依赖性，而对猪兽疫链球菌呈现部分浓度依赖性。

Through comparison of 16S rRNA gene sequence of Riemerella anatipestifer serotype 1 to 19 with that of Escherichia coli,Salmonella,Pasteurella and Staphylococci,a pair of specific primers was designed.

根据 GenBank中的鸭疫里默氏杆菌1～19型16S rRNA基因序列，分别与大肠杆菌、沙门氏菌、葡萄球菌、巴氏杆菌的16S rRNA基因序列对比，设计特异性引物，建立了基于鸭疫里默氏杆菌16S rRNA的PCR检测方法。

Secondly, the rate of infection of the Pasteurella multocida reached to 16%(12/75), Riemerella anatipestifer bacilliis also high, the ratio was 12%(9 / 75). More over, hemolytic bus bacteria, Salmonella and Staphylococcus aureus were also showed in the poultry tested.

结果表明：大肠杆菌仍是该部分地区危害家禽的主要致病菌，它的个体感染率高达41.3%（31/75）；其次是多杀性巴氏杆菌，个体感染率为16%(12/75)；鸭疫里默氏杆菌的个体感染率也较高，为12%（9/75）；此外还分离到溶血性巴氏杆菌、沙门氏菌和葡萄球菌。

Additionally, 75 volatile chemical constituents have been identified from the petroleum ether extracted part by capillary GC-MS, and the relative percentages of them are determined by GC method. The result showed that the volatile constituents of Musella lasiocarpa mainly includes advanced alkane, aliphatic acid and ester, aldehyde, ketone, aromatic and its heterocyclic compounds. With the compounds that have been isolated and identified from Musella lasiocarpa, the biogenensis pathway of new compound MT-3 is approached. Furthermore, the bacteriostasis of the 6 compounds to Escherichia coil, Pseudomonas aerusinosa, Bacillus megaterium, Sarcina lutea and Bacillus subtilic are determined by flat dilution method.

此外，从其石油醚萃取部分用毛细管色谱法分析鉴定出75种挥发性化学成分，并测定了各组分的相对百分含量，结果表明，地涌金莲挥发性化学成分以高级烷烃、高级脂肪酸酯及醛、酮、芳香和芳香杂环化合物为主；根据从地涌金莲中鉴出和分离得到的化合物，探讨了新结构化合物MT-3的生源代谢途径；采用平板稀释法测定了地涌金莲中六种化学成分对大肠杆菌、绿脓杆菌、巨大芽孢杆菌、藤黄八叠球菌和枯草芽孢杆菌的抑菌作用。

In view of these facts, We tried to find something in the L-form induction, plasmid extraction and their function, and the cloning and expression of promotors from BCG in Bacillus subtilis. Our aim is to provide a basis for the expression of HBsAg gene in BCG and to add something to the molecuiar biology of mycobacteria.

正是有鉴于此，我们试图在分枝杆菌在液体培养基中的L型实验诱导、质粒的提取及其功能分析、卡介苗启动子在枯草杆菌的克隆和表达等方面有所发现，为利用卡介苗表达乙型肝炎表面抗原基因提供基础，也为我国分枝杆菌的分子生物学研究增加内容。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。