杆菌的

Sequences analysis revealsthat these 54 strains have as high as a 99% similarity in 16S rRNA gene level. Allthese 54 strains could be classified into six subgroups according to six differentregions on full-length 16S rRNA gene. Even if the regional differences are not evidentto distinguish B. thuringiensis from B. cereus, however the data of the 16S rRNA genesequences will serve to broaden the database for Bacillus, and provide someinformation for the classification of genus of Bacillus. Additionally, seven B.

本研究利用通用引物，对54株苏云金芽胞杆菌和蜡状芽胞杆菌的16S rRNA基因进行测序，并对所得到的序列进行简单的分析，发现苏云金芽胞杆菌和蜡状芽胞杆菌的16S rRNA基因的相似性很大，大约为99％，在其全长含有6个区域的不同，根据这6个区域将Bt和Bc分成6个亚群，而Bt和Bc在不同亚群之间交替存在，说明16S rRNA基因上的差异不足以区分Bt和Bc。

According to their individual shapes, they are Gram positive, catenulate rod-shaped bacteria that can produce elliptical endospore in the middle position. These characteristics are similar to Bacillus cereus' described in Bergey's Manual of Systematic Bacteriology. And the colony States, physiological and biochemical characteristics of the two strains also accord with Bacillus cereus', which suggests that they might be identified as Bacillus cereus.

通过菌株的个体形态观察，发现这两株菌是可以在细胞中间产生不膨胀的椭圆形芽孢的革兰氏阳性杆菌，且相互成链，这与伯杰氏手册中对蜡状芽孢杆菌的描述相似，另外对这两株菌的培养特征、生理生化特征的研究，也表明这两株菌符合蜡状芽孢杆菌的特性，故初步鉴定这两株菌为蜡状芽孢杆菌。

Adopting duple diluent method and stiletto method and external antibacterial test on escherichia coli, solmonella paratyhpi , staphylicoccus aureus, streptococcus,, pseudomonas aeruginosa , Erysipelothrix rhusiopathiae, pasteurellosis to get the MIC and MBC, we studied antibacterial effect of the Copmounds Solatium Iyratum Thunb injection.

本研究采用试管二倍稀释法和打孔法对大肠杆菌、沙门氏菌、金黄色葡萄球菌、链球菌、绿脓杆菌、丹毒杆菌、巴氏杆菌的最小抑菌浓度和最小杀菌浓度及体外抑菌试验，研究复方白毛藤注射液抗菌作用。

In this study we build a Mycobacterium marinum random mutant libratory with MycoMarT7 transposon phage, found two cell wall virulence genes with colony morphology and cording structure strategy and build up virulence detection system in cell and animal model. We concluded that PDIM is critical for the virulence of Mycobacterium marinum.

本课题利用MycoMarT7噬菌体建立了海分枝杆菌随机突变库，以海分枝杆菌为模型筛选出两个分枝杆菌细胞壁毒力相关基因，并建立了海分枝杆菌的毒力检测系统，发现PDIM在海分枝杆菌的致病性中起到重要重要的作用。

Multiplex-PCR detection method for Pasteurella multocida and Haemophilus parasuis had been developed based on published single PCR for these pathogens. The PCR reagents, annealing temperature and the PCR procedure were optimized and the primers were screened, the PCR products including 457bp of PM and 1090bp of HPS were obtained just by one test.

在已经建立的多杀性巴氏杆菌、副猪嗜血杆菌的单项PCR诊断方法的基础上，通过对扩增条件和引物的筛选，成功地建立了PM、HPS复合PCR诊断方法，利用一次PCR反应，即可同时扩增多杀性巴氏杆菌的457 bp和副猪嗜血杆菌的1090 bp的特异性片段。

For example, it is a pathogenic bacterium and has endotoxins; it secretes protein into the periplasm and often into inclusion bodies. Bacillus subtilis is an attractive host for the production of heterologous secretory proteins. Many genes has succeed expressed in Bacillus subtilis, and the plasmid vectors for Bacillus subtilis are now taken from other genus bacterium, such as Staphylococcus aureus or lactococcus lactis and so on. As it is clear to known that these plasmids are far from optimal for Bacillus subtilis, because of low efficiencies of cloning and high levels of replicating instability.

枯草芽胞杆菌是目前原核表达系统中分泌表达外源蛋白较理想的宿主，近几年已在枯草芽胞杆菌中成功表达了多种基因，但大多都应用来源于金黄色葡萄球菌、乳酸杆菌等的载体来表达外源基因，由于其在枯草芽胞杆菌中存在分离复制的不稳定及外源基因的克隆表达效率低的现象，故都不是枯草芽胞杆菌的最佳选择，还有待改进。

It was found that 0.25%o potassium sorbate produced a positive inhibition against short G+ spore bacillus with a concentration less than 5xl04cfu/ml. A concentration less than %o potassium sorbate hardly exerted a complete control towards short G- plump bacillus having a population density of 5x104cfu/ml. It was proved that use of l% potassium sorbute never controlled the growth of G+ coccus, G~ spirilla and enterobacter with a population density of 104cfu/ml. 1mmol EDTA completely controlled the growth of G+ short spore bacillus and G+ coccus whose cell density was 5xl04cfu/ml. A level of lmmol EDTA showed a limited inhibition against the growth of G- spirilla with a population density of 105 cfu/ml. However, a level of 10mmol EDTA completely controlled the growth of the G- spiral bacteria having a population density of 105cfu/ml. lOmmol EDTA produced a very significant control towards the growth of G"" plump short bacillus with 105cfu/ml. 20mmol EDTA showed a remarkable inhibition against the enterobacter with a population density of 105cfu/ml. Different concentrations of nisin including 25mg/mL, 50mg/mL, 75mg/mL and 100mg/mL were used as bio-preservative to examine its effects against the growth of all strains leading to the spoilage of fresh mutton meat. It was seen that there was a big difference in nisin's concentrations in inhibiting the spoiling bacteria. Generally speaking, as more as 75mg/mL of nisin significantly inhibited the growth of G+ short spore bacillus, G-plump short bacillus, enterobacter, G'spiral bacteria and G+ coccus having a population density of 105cfu/ml.

分别运用山梨酸钾、EDTA和Nisin对7种主要引起羊肉腐败的微生物进行了抑菌实验，结果显示，0.25‰以上山梨酸钾能够有效抑制5×10~4 cfu／mL以下的革兰氏阳性短芽孢杆菌的生长；1‰以下的山梨酸钾不能完全抑制5×10~4 cfu／mL革兰氏阴性粗短杆菌的生长，对10~4 cfu／mL革兰氏阳性球菌菌株、革兰氏阴性螺旋菌菌株和肠杆菌菌株抑制效果不太明显。1mmoL EDTA能完全抑制住小于10~5 cfu／mL革兰氏阳性短芽孢杆菌菌株、革兰氏阳性球菌菌株的生长，能明显的抑制10~5 cfu／mL的革兰氏阴性粗短杆菌菌株生长，对10~5 cfu／mL的革兰氏阴性螺旋菌菌株有一定的抑制作用。10mmoL EDTA能完全抑制住10~5 cfu／mL革兰氏阴性螺旋菌菌株的生长；能明显抑制10~5 cfu／mL的革兰氏阴性粗短杆菌菌株的生长，而20 mmoL EDTA能很明显抑制10~5 cfu／mL肠杆菌菌株的生长。25mg／mL、50 mg／mL、75 mg／mL和100 mg／mL的Nisin几乎对所有引起羊肉的腐败菌有抑制作用，但抑制程度不同，抑菌活性有一定的变化。

Provided as a public service by Office of the Assistant Secretary of Defense-Public Affairs and the Anthrax Vaccine Immunization Program Agency, the AVIP web site reports information on anthrax including: the threat from anthrax, anthrax infection, anthrax vaccine and vaccination protection.

这是炭疽杆菌疫苗免疫项目的网站。它是由公共防御事件辅助办公室和美国炭疽杆菌疫苗免疫项目局共同创建并服务于大众，提供有关炭疽杆菌的信息，如：来自炭疽杆菌的威胁、炭疽杆菌感染、炭疽杆菌疫苗以及疫苗保护。

These are foundation stones of biological classification of the genus Bifidobacteria. The designation of species and biotypes is on the basis of carbohydrate fermentation physiological characteristics and DNA-DNA homology studies.

在婴儿和成人的肠道、阴道和口腔以及许多动物的消化器官中都可检出双岐杆菌，它们可分为人类种群、非人类种群，并具有不同的生物型。1双岐杆菌的形态双岐杆菌是无运动性不产芽孢的杆菌，在不同条件下呈现不同的形态。

The probe of chip includes the entire genomic DNA of at least ten thiobacillus ferrooxidans, at least two thiobacillus acidophilus sulfur oxides, at least one eosinophils thiobacillus, at least one acidithiobacillus albertensis bacteria, at least six thiobacillus oxidation of ferrous - Lo, at least eight heterotrophic bacteria acidophilus, at least one metal leaf sulfur bacteria, at least one Acidianus tengchongensis, at least a Metallosphaera sedula and at least two of thermophilic bacillus curing.

该芯片的探针包括至少10株氧化亚铁硫杆菌、至少2株嗜酸氧化硫硫杆菌、至少1株喜温嗜酸硫杆菌、至少1株Acidithiobacillus albertensis菌、至少6株氧化亚铁微螺菌、至少8株异养嗜酸菌、至少1株金属硫叶菌、至少1株嗜酸两面菌、至少1株勤奋金属球菌和至少2株嗜热硫化杆菌的全基因组DNA。这种芯片，能够检测酸性环境中丰度较低的微生物，具有高通量、同时、快速、准确、灵敏地分析酸性环境中微生物群落组成的优点。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。