杆菌性的

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

Compared with 11 other isolates reported in GenBank, there was slightly variation in the nucleotide sequence of Omp25 gene of wapiti's isolate. The identities of nucleotide sequence and deduced amino acid sequences among 11 strains were 99.1%-99.4% could 99.2%-99.5% respectively.

与GenBank中已经登录的布氏杆菌其他11个分离株相比，马鹿布氏杆菌分离株Omp25基因变异较小，以散在的点突变为主，Omp25基因核苷酸和推导氨基酸序列的同源性分别为99.1%-99.4%和99.2%-99.5%之间。

To understand the role of the mucosa-associated microbiota in pathogenicity of ulcerative colitis, paired biopsies were obtained during colonoscopy from the ulcerated and nonulcerated gut mucosa of 24 patients with UC. The dominant bacteria and four important groups lactic acid bacteria, Clostridium leptum subgroup, Bacteroides spp.

通过PCR-DGGE技术比较24例溃疡性结肠炎患者肠道内溃疡粘膜以及与溃疡粘膜相距5cm的非溃疡粘膜所黏附的优势菌以及拟杆菌、柔嫩梭菌、乳酸菌和双歧杆菌的组成情况，结果表明同一肠段两个部位的优势菌组成很相似，而柔嫩梭菌及乳酸菌的组成存在很大的差异。

Different plant expression vectors had been respectively transferred into upland cotton cultivars Jinman12, Jinmian7, Xinluzao1 and Jihe321 via Agrobacterium tumfaciens transformation. These vectors carried cryIAc3 gene, which encodes Bacillus thuringiensis δ-endotoxin, under the control of chimeric OM promoter, CaMV 35S promoter, and cotton leaf curl virus RP promoter respectively; snowdrop lectin gene (Galanthus nivals agglutinin, gna) trans-regulated by rolC promoter controlled TGAla factor; and multivalent expression construct of both cryIAc3-cpti fusion gene and gna gene. A large number of transgenic plants and their progenies had been obtained.

为了提高外源基因的表达量，延缓害虫产生耐受性，本文通过将携带有分别在高效复合OM启动子，CaMV35S启动子及棉花曲叶病毒RP启动子控制下的苏云金杆菌杀虫毒蛋白基因cryIAc3；反式调节因子增强韧皮部表达的雪花莲外源凝集素(Galanthus nivals agglutinin，GNA)基因；以及同时含有cryIAc3、豇豆胰蛋白酶抑制剂基因和gna三价抗虫基因的植物表达载体，以根农杆菌介导法分别将这些表达载体导入了陆地棉新陆早1号、晋棉7号、晋棉12号和冀合321等我国西北棉花主要栽培品种，经体细胞胚再生获得了大批转基因再生植株。

We concluded that sputum cultures in the Taipei Hospital Branches for Communicable Disease Control revealed that 4.06% were multi-drug resistant strains of Mycobacterium tuberculosis and a very high percentage of multi-drug resistance in Nontuberculous Mycobacterium, which requires the attention of health workers.

研究分析结果显示台北市立联合医院疾病管制院区所培养分离的菌株中结核分枝杆菌多重性抗药菌比例达4.06%，但非典型结核分枝杆菌抗药性之菌株比例却非常的高，值得密切的注意。

The results showed that the main pathogenic bacteria ledlocal cows to endometritis were streptococcus,the next,Escherichia coli,staphylococcus and pseudomonas aeruginosa.NFPL could quickly reduce thenumber of the bacteria in uterus,and strongerly inhibit these bacteria in vitro.NFPL could excit the uterus,which occurred in enlarging contractionamplitude.NFPL could lower abnormal high content of progesterone and raiseabnormal low oestradiol.

结果表明，引起本地奶牛子宫内膜炎的主要致病菌是链球菌，其次是大肠埃希氏杆菌，葡萄球菌，绿脓杆菌；新促孕液能能迅速减少病牛子宫内细菌的数量，在体外对这些细菌也有较强的抑制作用；新促孕液对兔子宫有明显的兴奋性作用，主要表现在收缩波幅的加大；新促孕液能降低病牛血清异常升高的孕酮含量，升高异常降低的雌二醇含量。

The results showed that the 16S rDNA copies of Bacteroides spp. decreased in rotavirus-infected children by 2 orders of magnitudes. 665 clones in Group H and 284 clones in Group R were sequenced and 34 OTUs were obtained according to 98% sequence homology. Partial least squares discriminant analysis of the clone library profiling data revealed significant structural shifts of gut microbiota.

利用偏最小二乘判别法(Partial least squares discriminant analysis，PLS-DA)对这34个OTU进行分析，结果表明轮状病毒感染导致肠道内拟杆菌组成结构发生了变化，通过拟杆菌的组成可以将健康个体与轮状病毒感染个体分开，而对于区分两组个体起关键作用的OTU分别与Bacteroides vulgatus ，Bacteroides stercoris和Bacteroides fragilis有很高的同源性。

Coli. The antimicrobial activity of the recombinant fusion protein was evaluated in vitro, which exhibited high antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, and Pasteurella multocida, but low antimicrobial activity against Escherichia coil, and no antimicrobial activity against Salmonell choleraesuis.

对该重组蛋白进行纯化与体外抗菌活性的测定。结果表明，重组鸡AvBD6融合蛋白对多杀性巴氏杆菌、金黄色葡萄球菌、枯草芽胞杆菌有较高抗菌活性，对大肠杆菌的抗菌活性较弱，对猪霍乱沙门氏菌无抗菌活性。

The results indicate that (1) After acclimation and induction, Phosphorus Accumulating Organism not only has the characteristics of denitrification, but also has the characteristics of takeup phosphate in aerobic;(2) Denitrifying phosphate accumulating bacteria using NO3- as electron acceptor indeed existed in the conventional enhanced biological phosphorus removal system;(3) The facultative anaerobe seperated during the process of acclimation are Corynebacterium, Pseudodiphtheria corynebacterium, Staphylococcus and Moraxella; all of which can be functioned as denitrifying phosphorus accumulating to various degree.

试验结果表明：(1)好氧聚磷菌经驯化诱导后不但具有反硝化产氮特性，而且具有好氧聚磷特性；(2)在传统的A2O 工艺中存在有以NO3-为电子受体的反硝化聚磷菌；(3)在驯化过程中分离到的兼性厌氧微生物有棒状杆菌属、假白喉棒杆菌、葡萄球菌属及莫拉氏菌属，且都有不同程度的反硝化聚磷功能。

Pathogeny of northeast tiger was separated and identified in Jilin City Jiangnan Park. In order to carry out the epidemiological investigation and provide the accuracy diagnose and treatment measures, toxA gene of pastuerella was researched in the experiment.

通过对吉林市江南公园送检东北虎病例的病原分离鉴定，分离出多杀性巴氏杆菌，为进一步加强对该病的流行病学调查，并为准确诊断和治疗该病提供依据，实验对该株巴氏杆菌毒力基因toxA进行了研究。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。