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There was no epitrochlear or axillary adenopathy.

对一个结节进行针吸活检,抽出了黏稠的化脓性液体,该液体的抗酸杆菌检查阳性,根据细菌培养确定为海分枝杆菌。

objective to study the curative effect of fulguration combining mycobacterium injection in treat ment of condyloma acuminatum.methods the patients with ca were treated by fulguration in large range and local mycobacterium injection under fulguration wound.results the recurrence rates were 20.59% and 58.82% respectively in the observation group and in the control group.

目的 探讨电灼联合局部注射草分枝杆菌治疗尖锐湿疣的效果。方法治疗组应用电灼大范围炭化疣体,并在创面即疣体的基底部注射草分枝杆菌。对照组单纯采用高频电灼治疗。结果两组愈后复发率分别为20.59%、58.82%,前者明显较后者低,两组比较差异有非常显著性( p <0.01)。

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能,本研究选择了MAP的主要保护性抗原Hsp65蛋白,以副结核分枝杆菌C-2株染色体DNA为模板,以hsp65基因的特异性引物进行PCR扩增,获得了1 626bp的hsp65基因,通过T-A克隆技术,将PCR产物克隆至pGEM-T Vector中,以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆,成功地构建出克隆质粒pGEM-T-hsp65,以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列,结果表明,所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致,两者核苷酸序列的同源性为99.1%,氨基酸序列的同源性为99.3%,表明该基因在副结核分枝杆菌中高度保守。

We provide evidence that general O-glycosylation systems are conserved among intestinal Bacteroides species and likely contribute to the predominance of Bacteroides in the human intestine.

我们提供证据表明,完整O-糖基化系统在肠道的拟杆菌属种中具有保守性,并可能有助于确立拟杆菌属在人肠道的数量优势。

We observed marophages pre-treated with ConA spread more rapidly on the well bottom compared with PBS injected controls under phase-contrast microscope. SEM of macrophages treated with ConA showed marked larger size and many surface ridges or ruffles and fine fingerlike processes or filopodia in comparison to controls, surface of which were smooth.

结果表明:①ConA处理后,倒置显微镜下可见Mφ粘附性增强;扫描电子显微镜下见其体积增大,指状突起增多;透射电子显微镜下见Mφ内线粒体等细胞器增多,伸出伪足包绕结核分枝杆菌并内吞,ConA活化的Mφ吞噬结核分枝杆菌数量增多,菌体的完整性受损。

The study of morphological, physiological and biochemical characteristics showed that the strain 32-11-2-2 was a slight halophilic strain; it had the morphological characteristics of a continent Bocillus and could produce amylase, fluidify glutin; and it also showed a positive reaction in the litmus milk test. The 16S rDNA analysis showed the strain 32-11-2-2 had a 98% homology with Bacillus subtilis.

结果:分离得到421株细菌,包括活性菌株54株,其中活性菌株32-11-2-2是一株弱嗜盐菌,具有陆地芽孢杆菌的形态特点,能产生胞外淀粉酶、过氧化氢酶,液化明胶、石蕊牛奶反应阳性,16S rDNA序列分析该菌株与枯草芽孢杆菌同源性达到98%以上。

The study of relation between hepatogenic peptic ulcer and portal hypertension,transmitter metabolic disturbance,hepatic insufficiency and infection;and the therapeutic principles of complications of peptic ulcer are described.Twenty four of 58 patients with hepatogenic peptic ulcer were examined by color Doppler ultrasound.Portal venous flow volume (24 cases) was 1060.9±96ml/min.

为探讨肝源性消化性溃疡的发病机理及其治疗原则,对58例肝源性消化性溃疡患者采用了彩色多普勒超声仪连续监测其门静脉血流量(24例),测定门静脉压力(8例)、血中组织胺浓度(8例)、溃疡灶旁胃泌素含量(9例)及幽门螺杆菌(58例)。58例中经手术治疗13例,非手术治疗45例。

Objective To establish the PCR method for detection of Helicobacter pylori DNA in tissue embeded by paraffine and to investigate the relationship between Hp and recurrent oral ulcer.

目的 建立检测石蜡切片中幽门螺杆菌的PCR方法,明确复发性口腔溃疡患者口腔中是否存在幽门螺杆菌。

This method could amplify the specific fragment in the reference strains, but not in chicken E. coli, Haemophilus paragallinarum, Salmonella Pullorum and Avian pasteurella multocida, of ORT. A sensitivity test indicated that the assay's lowest detection limit was 90 pg DNA.

该方法能在ORT参考菌株中扩增到特异性片段,而鸡大肠杆菌、副鸡嗜血杆菌、鸡白痢沙门氏菌、禽多杀性巴氏杆菌的扩增结果均为阴性;敏感性试验表明该方法最低检出限量为90 pg DNA。

Re-sults:it was shown that Nano MgO had excellent germicidal efficacy to the Escherichia coli and Staphylococcus aureus.To such spore as Bacillus subtilis var.niger spore,Nano MgO also produced good germicidal effect.It was proposed that abrasiveness,alka-linity,electrostatic attraction,and oxidizing power of nano MgO all combined to promote these biocidal properties.

结果: ①纳米氧化镁能有效杀灭大肠杆菌,对枯草杆菌黑色变种芽孢也有显著的杀灭效果;②纳米氧化镁对金黄色葡萄球菌和枯草杆菌黑色变种芽孢有很好的抑菌作用;③纳米氧化物的磨蚀性、碱性、氧化性以及对微生物的静电吸附使它具有抑菌和杀菌作用。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。