杆菌性的

Real-time fluorescence quantitative PCR technique has been widely applied, such as mRNA expression, detections of DNA copy number and determination of mononucleotide polymorphism, as well as in the clinical medicine including accurate quantitative examination of mycobacterium tuberculosis, Type B and Type C hepatitis, AIDS virus, gonococcus, and chlamydia trachomatis.

实时荧光定量PCR的应用范围非常广泛，包括mRNA表达的研究、DNA拷贝数的检测、单核苷酸多态性的测定等，已广泛应用于医学临床，它能对结核分枝杆菌、乙型、丙型肝炎、爱滋病病毒、淋球菌、沙眼衣原体等病原体进行准确的定量检测。

Compartmentalization of Na~+ in vacuole is an important mechanism for plant, especially halophyte, to reduce the toxicity of Na~+.

自1983年第一株转基因植物的获得，开始了利用转基因技术改良植物种性的时代；1985年Horsh等人首创了叶盘转化法，使得农杆菌转化过程大为简化，从此植物基因转化研究得到迅速发展。

Bovine paratuberculosis is caused by Mycobacterium paratuberculosis and it is a kind ofchronic wasting disease in ruminants globally, which is called Johnes disease.

副结核病是由副结核分枝杆菌引起的一种全球性以反刍动物为主的慢性、消耗性传染病，也称Johnes。

To construct the recombinant plasmid of protein 38kDa of M. tuberculosis and express, purificate and renaturate fusion protein 38kDa and to evaluate its potential value for TB serodiagnosis.

目的 构建结核分枝杆菌38kDa的重组质粒，在大肠杆菌中表达、纯化和复性重组蛋白，并评价其在结核病血清学诊断方面的价值。

Following purification by Ni affinity chromatography and stepwise renaturation by dialysis, rB7-B5 showed sound antimicrobial activity in porcine actinobacillus pleuropneumoniae and antibiotics-resistant E. coli. The present study provides basis for the research and development of novel antibacterial preparations.

以经Ni亲和层析柱纯化和多步透析法复性的rB7-B5，对猪胸膜肺炎放线杆菌和耐药性大肠杆菌具有很好的抑菌活性，本研究为新型抗菌制剂的研制和开发奠定了基础。

However, this algicidal substance was unstable at different acid and alkali concentration, it could improve algicidal activity at neutral, alkaline, but reduce algicidal activity at high acid concentration;(4) according to the studies on the mophorlogical characters, the physilogical, biochemical characters and the sequence analysis of 16S rDNA of strain W5, it was identified as a Enterobacter aerogenes.

但酸碱稳定性不如热稳定性强，在强酸条件下，溶藻活性有所降低，中碱性条件下可以增强其溶藻活性；此溶藻物质活性成分是水溶性的，不溶于常见的有机溶剂(4)通过形态学特征、生理生化试验以及16S rDNA序列分析，W5菌鉴定为产气肠杆菌。

The research isolated a strain of pantomorphic Gram-negative coccobacillus from the lung and amygdale of a pig, which has serious typical I Porcine Contagious pleuropneumonea, by use the plate of ox brain heart infusion bouillon and chocolate medium.

本研究采用以牛脑心浸液为基础的液体培养基和巧克力平板接种方法，从某猪场典型传染性胸膜肺炎症状猪的肺脏和扁桃体中分离到1株具有多形性的革兰氏阴性球杆菌。

By the partial purification of Arthrobacter nicotinovorus D-97 endocellular enzymes and the results of enzymatic reaction of enzymes of partition collection, it was found that enzymes related to trehalose synthesis were consisted of Trehalose-forming Enzyme and Trehalose-releasing Enzyme . Trehalose-forming Enzyme could convert maltooligosaccharides into non-reducing maltooligosyl trehalose by intramolecular transglycosylation, and Trehalose-releasing Enzyme could hydrolyze α-1, 4 linkage between the trehalose residue and the maltooligosaccharide residue to release trehalose and maltooligosaccharides with two glucose monomer lesser.

将食尼古丁节杆菌D-97胞内酶部分纯化并对各收集组分进行酶反应，发现其海藻糖合成酶系由海藻糖生成酶(Trehalose-forming Enzyme，TFE)和海藻糖释放酶(Trehalose-releasingEnzyme，TRE)组成，前者分子内转糖基将直链麦芽寡糖转化为没有还原性的麦芽寡糖基海藻糖，后者专一切割麦芽寡糖基海藻糖分子上麦芽寡糖基与海藻糖连接的α-1，4糖苷键，从而释放出海藻糖和减少了两个葡萄糖单体的麦芽寡糖。

The genus Bifidobacterium contains irregular, nonsporing, gram-positive rods that may be club-shaped or forked at the end .

双歧杆菌属中包括有不规则的，无芽孢的，G性的，它们可能是短棒或是末端分叉的。

An ELISA(enzyme-linked immunosorbent assay.) was developed for detection of bovine brucellosis by using LPS as antigen purified by Heating - phenol recommended by OIE (office international des epizootics).

采用世界动物卫生组织推荐的热酚法提取的脂多糖作为抗原，建立了牛布氏杆菌病的ELISA方法，对45份血清进行检测，证明本ELISA方法灵敏性较高，经阻断试验和交叉试验，证明特异性较好。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。