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To understand whether the GST can protect plant from the damage of ultra-violet radiation,a UV inducible GST gene was isolated from an Arabidopsis cDNA library.The plant expression vector containing the GST cDNA was constructed and transferred into Arabidopsis thaliana plants by the Agrobacterium-mediated vacuum method.Molecular genetic analyses showed that the overexpression of the UV inducible GST could increase the tolerance of the transgenic plants to UV radiation

为了阐明GST在紫外辐射下是否对植物有保护作用,以紫外强烈诱导表达的GST cDNA为探针,筛选拟南芥cDNA文库,获得了这种GST的全长cDNA;利用此cDNA构建植物表达载体,并通过农杆菌介导法转化拟南芥,使其在拟南芥中得到大量表达;通过对转基因植株的紫外辐射耐性分析,证实了该GST的过量表达可明显增强拟南芥对紫外辐射损伤作用的耐受性。

Seven inactivated tetravalent vaccines of Riemerella anatipestifer,of which three (1#,3#,7#) employed oil-emulsion,two(4#,6#) employed propolis,two(8#,10#) employed aluminum hydroxide as their basic adjuvants,were developed and compared by the methods as follows:challenges were performed by homologous virulent RA strains to measure their protective abilities;indirect enzyme-linked immunosorbent assay was used to monitor antibody production;lymphocyte transformation test was performed to study the expression of cell-mediated immunity.

本实验通过对供试鸭的攻毒保护试验、通过用ELISA方法对供试鸭特异性抗体消长规律的研究以及通过用~3H-TdR掺入法对供试鸭T细胞免疫水平的检测三个方面对所研制的七种不同佐剂类型的鸭疫里氏杆菌四价灭活苗的免疫原性进行了比较研究。

OBJECTIVE:Proton pump inhibitor is a new effective drugs for the treatment of peptic ulcer,it alternatively inhibits H+/K+-ATP coenzyme and secreetion of hydrochloric acid , sterilizes helicobacter pylori.This paper summarizes the aspect of clinical usage and evaluate their clinical efficacy and safety.

目的:质子泵抑制剂的问市开辟了药物对抗消化性溃疡的作用途径,其选择性地抑制体内H+/K+-ATP酶活性和酸的分泌,同时对胃幽门螺杆菌的感染也具良好的杀灭作用,本文对其进展和临床应用进一步评价。

Methods: The minimum inhibitory concentrationof Xipayiguyiy on P.gingivalis,P.intermedia and F.nucleatum was observed by agar dilution in vitro.Also,the effects of Xipayiguyinye on oral malodour challenged by cysteine was investigated on 10 volunteers in vivo.Results: It showed in this study the MIC of Xipayiguyiy for Pg and Fn was 1mg/mL,and for Pi was 0.5mg/mL.It was also found the Xipayiguyinye achieved significant effects on oral malodour challenged by cysteine.

采用琼脂稀释法,测定西帕依固龈液对牙龈卟啉单胞菌(Pg、中间普雷沃菌、具核梭杆菌的最低抑菌浓度;对10例志愿者采用自身前后对照试验,观察四种干预措施(无干预、清水、西帕依固龈液、口泰)对半胱氨酸激发性口臭的最大值水平、各时间点下降百分比及口气值的下降曲线的影响,评价西帕依固龈液防治口臭的效果。

The apxⅢA gene of Actinobacillus pleuropneumonie was amplified by PCR. The amplified DNA fragment 3 466bp was cloned into pMD18-T. After R.E. analysis and sequencing, the apxⅢA gene in pMD18-T was ligated into pBluescripⅡSK, the recombinant expression plasmid pET-28b/apxⅢA was constructed and analysed with R.E., the protein of apxⅢA gene expressed in E.coli BL21 was detected by Western blotting.

因胸膜肺炎放线杆菌的致病性主要是由毒素决定的,故参照猪胸膜肺炎放线杆血清2型菌株的序列(GenBankL1 2 1 4 5)设计了一对特异性引物,用PCR的方法扩增apxⅢA基因并得到了长 3 466bp的片段,然后将其克隆到pMD 1 8T中,经酶切鉴定和序列分析表明克隆是成功的;再将apxⅢA插入到原核表达载体pET 2 8b后,转化BL2 1 (DE3),在IPTG诱导下获得高效表达,经Westernblotting检测证实表达产物有活性。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。