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In this study, we developed the plant regeneration system of island cotton Gossypium barbadense L.

本研究通过诱导海岛棉下胚轴的体细胞胚胎发生建立了海岛棉的植株再生体系;并以新海16胚性愈伤组织为受体,通过农杆菌介导法进行了SATI基因的遗传转化,获得了转基因再生植株。

The experimntal results showed that the recombinant plasmid could be expressed in the COS-7 cells with a typical T-helper 1-dominanted immune response as demonstrated by IgG isotype analysis.

结果 扩增的BCSP31保护性抗原基因片断在牛、羊和猪布氏杆菌株中具有高度保守性,构建的BCSP31/pVAX1重组表达质粒在COS7细胞有目的蛋白抗原的表达。

Moisture level is critical at 50%~65%, thus ensuring that the osmotolerant lactobacilli are active and dominant.

含水量保持在50%~65%是至关重要的,只有这样的含水量下才能确保耐高渗透性乳酸杆菌的活跃性和主导地位。

According to one conserved sequences of phage GIL01 from Bacillus thuringiensis,a pair of primers was designed.With overnight cultivations of 35 B.t standard strains and one fermentative strain MZ1 as templates,PCR results showed that 16 strains,which may be lysogenic strains,produced the product with the size about 750 bp as anticipated.

根据苏云金杆菌(Bacillus thuringiensis, B.t)溶原性噬菌体GIL01(GenBank Accession Number: AJ536703)的同源序列设计一对引物,用35株B.t标准菌株和生产菌株MZ1(血清型H3a3b)的过夜培养物作模板,结果有16株菌株包括MZ1能扩增出大小约为750 bp的预期产物,推测这16株菌为溶原菌。

Compared with those in control group,the number of positive immunoreactivity cell in RSR and intestine increased and its intensity of immunoreactivity enhanced obviously after rabbit was infected with Pasteurella multocida.

免疫组织化学观察发现,产抗菌肽细胞在兔圆小囊的黏膜上皮、圆顶上皮以及淋巴组织的滤泡生发中心、圆顶区和帽区均有分布;在兔感染多杀性巴氏杆菌后,圆小囊及其他肠道组织中的抗菌肽免疫组织化学阳性细胞数量明显增多,阳性反应增强。

Objective: To investigate the possible role of spasmolytic polypeptide and extracellular signal regulated protein kinase in the pathogenesis of helicobacter pylori relaed gastritis.

目的:探讨幽门螺杆菌在Hp相关性胃炎的发病机制中对胃黏膜上皮细胞解痉多肽以及细胞外信息相关蛋白激酶表达的影响。方法:检测68例浅表性胃炎患者的活检组织标本。

From its the depart in splanchnic organization arrives bacterium of 2 individual plant, pass bacteriological method appraisal, one individual plant is deputy pig bloodsucking bacili, another individual plant is a pig streptococcic; will send check sample book have an inoculation to marc-145 cell, can observe cellular pathological changes, the pcr of virus fluid classics of results detects, for positive of blue ear ill viru

从其内脏组织中分离到2株细菌,通过细菌学方法鉴定,一株为副猪嗜血杆菌,另一株为猪链球菌;将送检样本接种到marc-145细胞,能观察到细胞病变,收获的病毒液经pcr检测,为蓝耳病病毒阳性;将送检的样本接种spf鸡胚,收获的鸡胚尿囊液有血凝性,血凝试验和血凝抑制试验鉴定为猪副黏病毒。

Resistance of Bacillus subtilis var. niger Spores Occluded in Water-insoluble Crystals to Three Sterilization AgentsJOHN E. DOYLE AND ROBERT R. ERNSTResearch Laboratory, Castle Company, Rochester, New York 14623The resistance to destruction of spores of Bacillus subtilis var. niger occluded in crystals of calcium carbonate and exposed to ethylene oxide and moist and dry heat was determined and compared with the destruction of unoccluded spores.

检测了封闭在碳酸钙晶体中的枯草杆菌黑色变种芽孢暴露于环氧乙烷、湿热和干热中的耐受性,并与未封闭孢子的破坏相比。

Microbial players include yeasts, molds, acetic acid bacteria, and bacilli with lactic acid bacteria and clostridia as the principal anaerobes.

微生物的活动包括酵母菌、霉菌、乙酸菌和杆菌的活动,乳酸菌和梭菌是主要的厌氧性微生物。

The present invention discloses a strain of broad spectrum bacillus thuringiensis, Hcy08-02, preserved in the Chinese typical culture article preserving center in Wuhan University with the CCTCC No.M202025. It has excellent fermentation property, contains several pesticidal protein encoding genes and has effective killing function to cotton bollworm, cabbage moth, small mottid willow moth, cabbage worm and other pests.

本发明公开了一株广谱苏云金芽孢杆菌Hcy08-02,在武汉大学中国典型培养物保藏中心保藏,编号:CCTCC NO:M202025,该菌株有优良的发酵性,含有多个杀虫蛋白质编码基因,对鳞翅目的棉铃虫、小菜蛾、甜菜夜蛾、菜青虫等害虫具有高效的毒杀作用。

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