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Commonly a sporadic disease, actinobacillosis occasionally occurs in high incidence.

放线杆菌病通常是一种散发性疾病,但偶尔也有高的发病率。

Commonly a sporadic disease, actinobacillosis occasionally occurs in high incidence

放线杆菌病通常是一种散发性疾病,但偶尔也有高的发病

Actinobacillosis develops insidiously in soft tissues.

放线杆菌病是在软组织中呈隐袭性发生的。

Cloning, expression and antigenic analysis of heat shock protein A gene of human Helicobacter pylori.

人幽门螺杆菌热休克蛋白A编码基因的克隆、表达及抗原性研究。

Results In 105 cases with PU,2 cases were complicated with RE and 74 cases were positive in Hp testing in mucosa of gastric pyloric antrum.

国内外对幽门螺杆菌和反流性食管炎的关系一直存在着分歧。

In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

为了研制副结核病敏感、特异的诊断试剂和新型、高效的预防制剂,尤其是DNA疫苗,本研究筛选了M.paratuberculosis主要保护性抗原SOD基因,以M.paratuberculosis C-2染色体DNA为模板,以SOD基因的特异性引物进行PCR扩增,获得了624bp的SOD基因,通过T-A克隆技术,将PCR产物克隆至pMD18-T Vector中,以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆,成功地构建出克隆质粒pMD18-T-SOD,序列测定及DNASTAR分析表明,所获得的M.paratuberculosis C-2 SOD基因与Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致,两者核苷酸序列的同源性为99%,氨基酸序列的同源性为99.5%,表明该基因在副结核分枝杆菌中是高度保守的。

AIM: To observe the eradicative effect of dioctahedral smectite combined with omeprazole on Helicobacter pylori and its efficacy in treatment of peptic ulcer.

目的:探索十六角蒙脱石和奥美拉唑合用的抗幽门螺旋杆菌与抗消化性溃疡的疗效。方法:将85例经胃镜及HP检查证实的HP阳性消化性溃疡病人随机分为3组。A组32例口服十六角蒙脱石3g,tid,加奥美拉唑20mg,qd.B组28例服十六角蒙脱石3g,tid.C组25例服奥美拉唑20mg,qd。

Results indicate that the number of bacteria grown in media LB, CSEA and WSA was 14.84×10^7, 10.27×10^7 and 6.91×10^7 CFU per gram dry soil, respectively, after 192 h of incubation. The diversity index of soil bacteria was the highest in WSA, while the lowest in LB. Certain similarity was discovered in bacteria community between the three media, i. e. the Jaccard index was 57.69% between LB and CSEA, 53.13% between LB and WSA and 66.67% between CSEA and WSA. Phylogenetic analysis suggests that the dominant bacterial groups in the soil belong to β-Proteobacteria,γ-Proteobacteria and Actinobacteria. The sequence of 16S rDNA of some OTUs was found to have high homology with Burkholderiaceae bacterium, Rhodococcus and Mycobacterium, so it is presumed that the cells of these bacteria can secrete Resuscitation-Promoting Factors, which may effectively improve culturability of soil microorganisms.

结果表明,LB、CSEA、WSA培养基192h后每g干土获得的细菌数量分别为14.84×10^7、10.27×10^7和6.91×10^7CFU,但微生物多样性指数以WSA为最高,LB多样性指数最低;三种培养基培养的细菌菌群有一定的相似性,LB和CSEA培养基间的Jaccard指数为57.69%,LB和WSA培养基之间为53.13%,而CSEA和WSA培养基的相似性指数达66.67%;16SrDNA测序结果表明,所获得的土壤细菌优势种群在分类方面主要属于γ-和β-变形杆菌以及放线菌亚门,其中某些OTUs中的16SrDNA序列与Burkholderiaceae bacterium、Rhodococcus和Mycobacterium属具有较高的同源性,推测其细胞能够分泌复苏促进因子,有效地提高土壤细菌的可培养性。

Progressive atrophic rhinitis is an important respiratory disease in swine industry, caused by toxigenic Pasteurella multocida. The OmpH of Pasteurella mumtocida is a major outer membrane protein involved in the pathogenesis and immunity. In this study the ompH gene of P.

传染性萎缩性鼻炎是养猪业中的一种重要的呼吸道传染病,产毒素多杀性巴氏杆菌是其重要的病原之一,外膜蛋白H在该菌的致病和免疫中发挥重要作用。

In this study, we have cloned and identified two full-length cDNAs of OsBIMK2 and OsBISERK1, which encoding MAPK and SERK, respectively, and associated with benzothiadiazole induced resistance.

本文克隆和鉴定了水稻中与苯并噻二唑(benzothiadiazole,BTH)诱导抗病性相关的编码MAPK的OsBIMK2基因和编码SERK的OsBISERK1基因全长cDNA;研究了OsBIMK2基因和OsBISERK1基因在水稻—稻瘟病菌亲和与非亲和互作过程中的表达模式;原核表达了OsBIMK2基因,获得重组OsBIMK2蛋白,并研究了重组OsBIMK2蛋白的生化特性;克隆了OsBIMK2基因的启动子序列,并运用农杆菌介导的瞬间表达系统分析鉴定了启动子中参与基因表达调控的可能顺式元件;将水稻OsBIMK2基因导入烟草,获得过量表达OsBIMK2基因的转基因植株,这些转基因植株中防卫反应基因PR-1组成型表达,并提高了抗病性。

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