杆菌

Firstly, normal mice was given orally by SM(qd×2d), and the dysbacteriosis were caused in them ,and then they were given orally by STM,these mice were formed experimental salmonellosis models ,finally these salmonellosis models were treated by bifidobacterium ,and count respectively the cfu/g of STM number in the mice excrement during different time of therapy,detect the index numbers of liver and spleen respectively, detect content of TNF-a in blood and in spleen respectively.

先用硫酸链霉素给正常小鼠灌胃2d，出现菌群失调症状，再用STM灌胃造成小鼠STM感染，使用两岐双岐杆菌液干预治疗STM感染小鼠，检测不同治疗时间各组小鼠粪便STM菌落数、肝脾指数、血及脾中TNF-a的含量。采用SPSS12.0统计软件包进行数据处理分析，统计学方法采用双因素重复测量资料的方差分析、单因素随机化设计的方差分析、卡方检验、多组均数比较的非参数秩和检验。结果：随着治疗天数的增加，STM菌落数逐渐减少，未干预组、生理盐水组及双歧杆菌组比较结果均为P＜0.01；组间比较，双歧杆菌组STM数下降最快。

Experiment showed that the kind of ginkgo extracts from outer seed capsule have significantly inhibition to 25 species of fungi, such as bacillus subtilis, E.

实验研究表明，银杏外种皮提取物对枯草芽孢杆菌、大肠杆菌、酵母菌、金黄色葡萄球菌、痢疾杆菌和绿脓杆菌等25种菌类均有明显的抑制作用。

The optimum pH value was 9.5, cane sugar of carbon source, yeast extract of nitrogen source, and sodium nitrate were conducive to enzyme production.

目前用于碱性蛋白酶的生产菌和研究对象主要是枯草芽孢杆菌和地衣芽孢杆菌及短小芽孢杆菌，新的产酶菌株目前

And unrooted trees were established. The accession numbers in GenBank of the sequences of 16S rDNA of the strains were followed by PFK1(DQ295034), PFK2(DQ295035), PFK3(DQ295036), LZCL(DQ295040), ZCS1(DQ295037), MMA1(DQ295041), MMA2(DQ295038), JSQ1(DQ295042), JSQ2(DQ295039), JSQ3(DQ295043). The Blast and phylogenetic tree analysis showed that

3将分离菌株进行形态、生理和糖发酵实验，结果表明这些表型鉴定的结论与16 S rRNA基因序列同源性分析结论一致，分离纯化5种产品的10株菌中仍有相同的3株菌与标签标注的不一致，即其中标签上分别标注为青春双歧杆菌和长双歧杆菌的两株菌鉴定结果均为动物双歧杆菌

Consider to make clear in recent years, second acid is calcic - Bowman does not move bacillus clinical bacterium of single born of the same parents of the detached rate that go up and verdigris holiday comparatives, have the tendency that year after year rises, the fundamental condition pathogenic bacteria that already became the infection inside the courtyard [1] , have to a variety of antibacterial content be able to bear or endure higher property of a medicine [1, 2] , for this, we are monitored and statistic analysed my courtyard to came 1998 2004 7 years a second acid is calcic - the clinical infection condition that Bowman does not change bacillus, to 14 kinds of antibacterial content be able to bear or endure property of a medicine and be able to bear or endure medical tendency, sum up a report to be as follows now. 1 material and method all data all come from 1.1 basic materials to came on January 1, 1998 my courtyard outpatient service was mixed on December 31, 2004 the specimen sending check of inpatient, via clinical microbiology the lab is fostered and classics appraisal is second acerbity calcium - Bowman does not move bacillus to affect case of illness, with clinical and commonly used antibacterial at the same time content makes the kill that medical quick detects. 1.2 bacteria appraisal is mixed medical quick experiment uses live thing VITEK32 of Mei Li dust is full automatic the bacterial appraisal of bacterial analysis system and its form a complete set gets stuck and medical quick card undertakes detecting, 1998~2000 year use GNI appraisal gets stuck, 2001~2004 uses GNI+ card.

近年探究表明，乙酸钙-鲍曼不动杆菌临床上的分离率和铜绿假单胞菌相当，并有逐年上升的趋向，已成为院内感染的重要条件致病菌[1]，对多种抗菌药物有较高耐药性[1，2]，为此，我们监测并统计分析了我院1998年至2004年七年间乙酸钙-鲍曼不动杆菌的临床感染情况，对14种抗菌药物的耐药性及耐药趋向，现总结报道如下。1材料和方法1.1基本资料所有资料均来自于1998年1月1日至2004年12月31日我院门诊和住院病人的送检标本，经临床微生物学实验室培养并经鉴定为乙酸钙-鲍曼不动杆菌感染病例，同时以临床常用抗菌药物作药敏检测的结果。1.2细菌鉴定和药敏试验采用生物梅里埃VITEK32全自动细菌分析系统及其配套的细菌鉴定卡和药敏卡进行检测，1998~2000年使用GNI鉴定卡，2001~2004使用GNI+卡。

In addition to air some of the Central Plains and some micro-organisms (non-pathogenic micro-organisms saprophytic, Bacillus, is Achromobacter, as well as a small number of Streptococcus actinomycetes, yeast and fungi, and so on), there may be from a human body More pathogenic micro-organisms such as Mycobacterium tuberculosis, diphtheria bacteria, Streptococcus, Staphylococcus aureus, meningococcal, influenza virus, measles virus, etc.

除大气中原有的一些微生物（非致病性的腐生微生物、芽胞杆菌属、无色杆菌属、细球菌属以及一些放线菌、酵母菌和真菌等）外，也可能存在来自人体的某些病原微生物（如结核杆菌、白喉杆菌、溶血性链球菌、金黄色葡萄球菌、脑膜炎球菌、感冒病毒、麻疹病毒等）成为空气传播疾病的病原。

Seven major bacterial isolates were obtained and purified. Their 16S rRNA genes were amplified by PCR for sequencing and their identities were determined with homology comparisons. Five of the seven isolates belong to Actinomycetales including Kocuria KD139, Rhodococcus KD140, Rhodococcus KD142, Arthrobacter KD230, and Arthrobacter KD232; one is classified as Bacillus d KD178; and another one as Stenotrophomonas KD237. The phylogenetic tree was also constructed based on the analysis of 16S rRNA gene sequences.

通过对分离菌株的16S rRNA基因序列进行分析，发现其中5株细菌分别属于放线菌目的考克氏菌属(KD139)、红球菌属(KD140和KD142)和节杆菌属(KD230和KD232)， 1株细菌属于杆菌目的芽胞杆菌d属(KD178)，另外1株细菌属于黄色单孢菌目的寡食单胞菌属(KD237)；同时我们构建了系统进化树，确定分离菌株的相对进化地位。

Results CTX-M type ESBLs were detected from Escherichia colt, Klebsiella pneumoniae, Enterobacter cloacae, Enterobacter aerogenes, Citrobacter freundii and Providencia stuartii, this was the first detection of CTX-M-14 in Providencia stuartii.

结果 从大肠埃希菌、肺炎克雷伯菌、阴沟肠杆菌、产气肠杆菌、弗劳地柠檬酸杆菌和斯氏普罗威登斯菌中检出CTX-M型ESBLs，首次从斯氏普罗威登斯菌中检出CTX-M-14型ESBLs。

The results demonstrated that 22 of 50 were positive by PCR-ELISA method while only 7/50 and 18/50 by antiacid staining and culture respectively, and no positive result was found in 20 sputum specimens without Mycobacterium tuberculosis by the methods; Conclusion The pCR-ELISA method can objectively detect Mycobacterium tuberculosis from clinical specimens with high specificity and sensitivity.

对50份来自结核病人高度怀疑有结核分枝杆菌的标本检测表明，PCR-ELISA检出22份阳性，比抗酸染色法(7/50)、培养法(13/50)和PCR-电泳法(18/50)检出率高，而20份证实无结核分枝杆菌的标本，几种方法检查均为阴性。结论该法可敏感、特异和客观地检测临床标本中的结核分枝杆菌。

According to the identification, Bacillus subtilis CGMCC No.1982 is a typical Bacillus subtilis. Compared with other Bacillus subtilises, Bacillus subtilis CGMCC No.1982 has broad-spectrum antipathogen fungal activity; the fermentation broth and spore have sound effect in preservation of fresh fruits such as peach, grapes, etc, and also have sound antiviral effect in production of cucumber, tomato and other greenhouse vegetables.

经鉴定枯草芽孢杆菌CGMCC No.1982属于典型的枯草芽孢杆菌，与其它枯草芽孢杆菌相比其具有广谱抗病原真菌活性，其发酵液和芽孢对鲜桃、葡萄等水果保鲜效果较好；用于黄瓜、番茄等大棚蔬菜的生产抗病效果较好。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。