杆菌

Fluorescent instruments.the colonies of the specimens yielding growth were identified by the vitek32 instruments and kb method was used for the drug sensitivity tests.results three hundred and thirtyone bacteria strains were isolated from a total of 3104 blood culture specimens,with a positive rate of 10.7%.of the identified bacteria,g+ cocci accounted for 50.3%,g-bacilli accounted for 44.7%,fungi accounted for 3.0%,and anaerobian accounted for 1.1%.the susceptibility rates of g-bacilli to imipenem,amikacin and cefoperazone/sulbactam were high.the susceptibility rate of g+ cocci to vancomycin and imipenem were also high.conclusion g+ cocci prevail over g-bacilli in the blood specimens.the staphylococci are the chief bacteria in the childrens blood specimens.both g+ cocci and g-bacilli are sensitive to imipenem.

结果 在3 104份血液培养标本中分离出病原菌331株，阳性检出率为10.7%。病原菌以革兰阳性需氧球菌居首位（50.3%），革兰阴性需氧杆菌次之（44.7%），真菌占3.0%，厌氧菌占1.1%。血液培养中的g+球菌对万古霉素和亚胺培南较为敏感，g-杆菌对亚胺培南、舒普深、丁胺卡那较为敏感。结论血液培养病原菌以g+球菌为主，g-杆菌次之；儿童血液培养病原菌以葡萄球菌属为主；亚胺培南对g+球菌和g-杆菌均具有较高的敏感率。

Responsible for constructional planning of clinical application research, and its implementation, along with the examination studies on intestinal tract micro ecology and hematological attributes of AIDS patients, which including the quantitative tests of Bifidobacterium, Lactobacillus, Bacteroides, Enterobacter, Escherichia coli and cl.perfringen in feces.

负责临床应用总体设计和临床应用研究，负责艾滋病人血液指标及肠道微生态学检测研究，其中包括：粪便中双歧杆菌、乳杆菌、拟杆菌、肠杆菌、肠球菌、产气荚膜梭菌数量的检测。

After avilamycin 8mg/kg was fed to Cyprinus carpio, the quantity of the intestinal aerobic and the facultative anaerobic bacteria, the percent of Enterobacteriaceae, and the quantity of Lactobacillus decreased significantly on the 14~ d, at the same time , the percent of Flavobacterium increased evidently, the quantity and percent of Bacteriodes decreased evidently during the first 28 d of the experiment.3 After Cyprinus carpio was fed with narasin, the percent of Corynebacterium increased.

投喂阿维拉霉素8mg／kg在试验第14d使鲤肠道好氧及兼性厌氧菌总数、肠杆菌科所占百分比、乳杆菌属数量显著降低，黄杆菌属所占百分比显著升高；在试验的前28d使拟杆菌属数量及其百分比显著下降。

The detection rates of methicillin-resistant S. aureus and penicillin-nonsusceptible S. pneumoniae were 15.9% and 84.3%, respectively. The prevalence of ESBLs-producing strains in Klebsiella pneumoniae, Escherichia coli, Serratia spp and Enterobacter cloacae were 31.2%, 46.2%, 94.8% and 16.8% respectively. The resistance rates of Hemophilus influenzae and Hemophilus parainfluenzae to ampicilin were 40% and 36% respectively.

耐甲氧西林金黄色葡萄球菌检出率为15.9%；对青霉素不敏感的肺炎链球菌（包括PISP和PRSP）检出率为84.3%；肺炎克雷伯菌、大肠埃希菌、粘质沙雷菌和阴沟肠杆菌产ESBLs的检出率分别为31.2%、46.2%、94.8%和16.8%；流感嗜血杆菌和副流感嗜血杆菌对氨苄西林的耐药率为36%和40%；铜绿假单胞菌和鲍曼复合不动杆菌对亚胺培南的耐药率分别为10.7%和13.2%。

The results showed that the lactic acid bacteria were belongs to those special homotype fermention types of Lactobacillus.9 strains belong to Lactobacillus gasseri, one does Lactobacillus.salivarius and the other 9strains do Lactobacillus.crispatus.

其中1-1、1-2、1-3、1-7、51-1、51-4、52-1-1、52-1-2、52-3 共9 株为格氏乳杆菌， 23-2-1 为唾液乳杆菌(Lactobacillus.salivarius)，23-2-2、23-4、23-5、23-8、32-3、46-1、46-2、46-3、50-3 共9 株为卷曲乳杆菌(Lactobacillus.crispatus)。这19 株乳杆菌发酵过程中都产生细菌素，除了32-3，51-1，51-4 这3 株菌外，都产生过氧化氢。

The biotransformations of isoeugenol to vanillin with various strains available in our lab were tested. It was found that several baterica including Bacillus subtilus 7658, Alcaligenes AS1.7617, Brevibacterium flavum 1.7a and Brevibacterium flavum AS1.013 could convert isoeugenol to vanillin.

对实验室所保藏的细菌、放线菌、真菌进行初步筛选发现细菌中枯草芽孢杆菌7658、粪产碱杆菌AS1.7617、黄色短杆菌1.7a、黄色短杆菌AS1.013能产生少量香草醛，最高浓度为0.31 g/L；放线菌中诺卡氏菌Nocardia sp。

Unsurprisely,These three microorganisms decreased as the storage time increases.84 strains lactobacillus were isolated during the detection period in the pickled gingers stored in low temperature.By physiology and biochemistry methods,32 strains L.plantarums,6 strains L.brevis,2 strains Lacto...

低温保存泡姜在整个检测期内，共分离到84株典型乳酸菌，经生理生化实验鉴定出植物乳杆菌32株、弯曲乳杆菌7株、短乳杆菌6株、肠膜明串珠菌属5株、乳球菌属2株、乳杆菌属32株；酵母菌分离鉴定出3个属，主要为酵母属以及少量的假丝酵母属和汉逊氏酵母属；只在保存的前2d检测到个别的青霉属和毛霉属；在整个检测期内，没有分离到大肠杆菌。

Multiplex-PCR detection method for Pasteurella multocida and Haemophilus parasuis had been developed based on published single PCR for these pathogens. The PCR reagents, annealing temperature and the PCR procedure were optimized and the primers were screened, the PCR products including 457bp of PM and 1090bp of HPS were obtained just by one test.

在已经建立的多杀性巴氏杆菌、副猪嗜血杆菌的单项PCR诊断方法的基础上，通过对扩增条件和引物的筛选，成功地建立了PM、HPS复合PCR诊断方法，利用一次PCR反应，即可同时扩增多杀性巴氏杆菌的457 bp和副猪嗜血杆菌的1090 bp的特异性片段。

These are foundation stones of biological classification of the genus Bifidobacteria. The designation of species and biotypes is on the basis of carbohydrate fermentation physiological characteristics and DNA-DNA homology studies.

在婴儿和成人的肠道、阴道和口腔以及许多动物的消化器官中都可检出双岐杆菌，它们可分为人类种群、非人类种群，并具有不同的生物型。1双岐杆菌的形态双岐杆菌是无运动性不产芽孢的杆菌，在不同条件下呈现不同的形态。

The fusion DNA fragments of ag85b-mpb64 and ag85b-mpb64-esat-6 were obtained by PCR andSOE technique. Various DNA vaccines were constructed with the pcDNA3.1: fusion of two genes, and of three genes, bivalent combinations and trivalent combinations(pCA+pCM+pCE6). BALB/c mice were vaccinated with this DNA vaccines.The mice injected withBCG were positive control and the mice injected with pCDNA3.1 and PBS were negative control.The mice were immunized 3 times with 2-wk intervals. The animals in group BCG were only inoculatedsubcutaneously with 1×10~6 CFU BCG at initial vaccination. The serum IgG titers and IgG isotype weredetermined using iELISA coated with M. bovis PPD and rMAE protein expressed and depurated inprokaryotic expression system every week.

同样，利用PCR和SOE技术，获得牛分枝杆菌mpb64-ag85b和mpb64-ag85b-esat-6融合基因，以pCDNA3.1为载体构建了牛分枝杆菌多价组合和多基因融合DNA疫苗：二基因融合(pCDNA3.1-MPB64-Ag85B，简称pCMA)和三基因融合(pCDNA3.1-MPB64-Ag85B-ESAT-6，简称pCMAE)DNA疫苗；二价组合和三价组合(pCA+pCM+pCE6)DNA疫苗，免疫BALB／c小鼠，以牛分枝杆菌BCG免疫组为阳性对照，以pCDNA3.1及PBS免疫组为阴性对照，共免疫3次，每次间隔2周，BCG组仅初免时皮下免疫1次。1免后每周，以原核表达纯化的重组MPB64-Ag85B-ESAT-6蛋白和牛分枝杆菌PPD为包被抗原，以间接ELISA方法检测血清IgG水平及lgG亚类。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。