杆菌

Objective To establish a new rapid method for the diagnosis of Brucella abortus.

目的建立一种快速诊断牛布鲁氏杆菌病抗原的新方法。

These results indicated that the BP26 protein can be used as the coating antigen in indirect ELISA test for the serological diagnosis of B. abortus infection in cattle.

abortus天然缺失OMP31基因），BP26作为间接ELISA包被抗原用于牛布氏杆菌血清学检测具有良好的特异性及较好的敏感性。

In order to cluone, seauence and express the outer membrane protein 25 (Omp25) gene sequence of wapiti's Brucella abortus strain ML.

目的 克隆马鹿布氏杆菌ML分离株25kDa外膜蛋白(Omp25)基因，并在大肠杆菌中表达。

The Omp25 gene of wapiti's Brucella abortus was amplified by RT-PCR and sequenced, Then the interesting gene was subcloned into pET28a for expression. The results showed that the full length of Omp25 gene was 642bp, which encoded 213 amino acids.

运用RT-PCR技术从马鹿布氏杆菌分离株中扩增出Omp25基因，将其克隆入pMD18-T中进行核苷酸序列测定和遗传变异分析，并将目的基因亚克隆到大肠杆菌表达载体pET28a中诱导表达。

Several antigens of pathogenic microorganisms, such as C subunit of tetanus toxin and the Brucella abortus ribosomal protein L7/L12, were successfully expressed in Lactococcus lactis.

破伤风毒素片段C、布氏杆菌L7／L12蛋白等多种病原微生物抗原已成功在乳酸乳球菌中表达，并已证明部分重组乳酸乳球菌作为黏膜免疫疫苗可以同时刺激局部黏膜免疫应答和系统免疫应答。

In principle, the protocol developed in this study could be used for detecting other foodborne pathogenic bacteria such as E.coli, Staphylococcus, Campylobacter and food toxins such as Ricin, Abrin, or Clostridium Botulinum if the proper antibody is selected for the conjugation with nanorod substrates.

大致上，这项研究兴盛的草案可以通过选择性结合在纳米棒底层的相对性抗体被用于检测另外一些食源性病原菌，比如大肠盖希氏菌，葡萄球菌，弯曲杆菌和食物毒素，诸如蓖麻毒素，红豆因或肉毒梭菌。

Nigra during the experiment. Successfully established the transformation procedure Agrobacterium-mediated of Populus simonii×P nigra: cut vigorous leaves to 2-3 patches through the main vein after growing about 20 days, pre-culture time was 3~4days;centrifuge Agrobacterium when being developped to the logarithm growth timeOD_(600=0.5, then dilute Agrobacterium to the concentration of OD_(600)=0.3-0.4 with MS liquid culture medium or asepsis distilled water for infection;infection time was 6-15 minute;after being co-cultured 3 days, inoculate on the selecting medium to be filtrated in succession during inducing unsureness buds and rooting with Kanamycin(containing Kanamycin 40mg/L and Cefazolin Sodium 700mg/L).

成功摸索出农杆菌介导的小黑杨遗传转化体系：选取生长20d左右的健壮的小黑杨组培苗叶片，通过主叶脉剪2～3下，预培养3～4d；将培养至对数生长期的农杆菌OD_(600=0.5离心后用液体MS培养基或无菌蒸馏水稀释成OD_(600)=0.3～0.4浓度，进行浸染；侵染时间为6～15min；共培养3d后，转入到筛选培养基中进行诱导不定芽及生根阶段的卡那霉素连续筛选(含有40mg/L卡那霉素和700mg/L的头孢唑林钠)。

Of or relating to or produced by the botulinus.

属于、关于肉毒杆菌的，或由肉毒杆菌产生的。

Methods There 2 395 patients from ten counties or cities in Han group in Hainan at gastrointestinal out-patient department of the hospital were detected by rapid urease test of H.pylori and gastroscope.

目的 调查海南汉区在消化内科就诊患者中幽门螺杆菌（Helicobacter pylori，H.pylori）感染患病情况，以及幽门螺杆菌感染与胃黏膜病理的关系。

Methods there 2 395 patients from ten counties or cities in han group in hainan at gastrointestinal out-patient department of the hospital were detected by rapid urease test of h.pylori and gastroscope. the posititives of rapid urease test of h.pylori were determined with 14c-urea breath test (14c-ubt).two positive tests were confirmed to be infected with h.pylori.

选择海南汉区10个县市2 395例具有上消化道症状就诊患者，经胃镜检查并取胃窦黏膜做h.pylori快速尿素酶试验，阳性患者做14c呼气试验，两者阳性确定为幽门螺杆菌感染。1 004例幽门螺杆菌阳性患者取胃窦及病变部位胃黏膜做病理组织检查。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。