杆菌

Probiotics is a living microbial ecological agent composed by lactobacillus,bifidobacterium,streptococcus sporolactobacillus and saccharomycetes through special technology.

益生菌是由乳杆菌、双歧杆菌、革兰氏阳性菌、酵母菌等经过特殊工艺复合而成的活体微生物制剂，是近年来新兴的高科技热门产品，风靡全世界。

The antimicrobial activities were determined with paper disc diffusion method. The result showed that of Sapindus mukorossi extracts only inhibited Candida albicans and no antimicrobial activities to Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Pityrosporum ovale and Propionibacterium acnes.

配制无患子溶液进行抗菌试验，以圆盘滤纸扩散法分析，结果显示甲、乙、丙、丁醇溶液样品，唯对白色念珠菌具抑制效果，而对另外5种菌种(大肠杆菌、金黄色葡萄球菌、绿脓杆菌、皮屑芽孢菌、痤疮杆菌)无明显抗菌效果。

For further understanding the effect of the strain on K-bearing silicate mineral, an experiment with KO2 strain cultured in nitrogen-containing and nitrogen-free medium was conducted. By putting mineral powders into the medium and measuring the concentration of capsular polysaccharides, we concluded that the strain can accelerate the weathering rate of silicate minerals. The results showed that the polysaccharides secreted by the strain in the growth process could help the bacterial to adhere to the mineral surface effectively, and created the bacterium-mineral complex, which formed a mircro-enviorment avail the release of potassium. Another result was that there was higher level of Carbonic Anhydrase activity, which revealed that some exo-protein or enzyme produced by the bacterial had postive impact on the process of releasing potassium ion. We carried out the bacterial fermentation for larger scale production of the bacterial secretion, which was used to sperate, and identify the small molecules related to the mineral-bacterial interaction. After the analysis, it was found that the strain can produce many kinds of small molecules, such as 2-Hydroxybenzoic acid, 4-Hydroxyphenylacetic acid, methyl-4-hydroxybenzoate, etc.

分别将菌株接入含有不同矿粉的培养基中培养，检测其在有氮、无氮培养基中荚膜多糖含量的变化，并在以钾长石和黑云母为矿源的情况下，比较研究了菌株在有氮、无氮培养基中对矿物的风化能力，通过一系列的实验，证实胶质芽孢杆菌在生长过程中所分泌的粘稠胞外聚糖可帮助细菌有效黏附在矿物表面，形成细菌-矿物复合体，并改变及维持该复合体内部的微环境，有助于该菌的解钾作用，而且细菌分泌的胞外蛋白质在该菌对含钾硅酸盐矿物的解钾作用过程中发挥重要作用；将胶质芽孢杆菌接入以钾长石和黑云母为矿源的有氮、无氮培养基中培养，检测到该菌碳酸酐酶活性的变化，并进行批量发酵后小分子酸性分泌产物的提取、分离、纯化与鉴定等方面的研究，结果表明，该菌可产生2-羟基苯甲酸，4-羟基苯乙酸，4-羟基苯甲酸甲酯等小分子物质。

OBJECTIVE To investigate the antiseptic resistance and Sulphonamide Compounds gene qacE△1-sul in multidrug resistance of Gram negative bacteria caused nosocomial infection,which aim to provides the basis to control nosocomial infection better.METHODS Multidrug resistant Gram negative bacteria were isolated from clinical.

目的了解多重耐药革兰阴性杆菌耐药现状和耐消毒剂磺胺复合物基因qacE△1-sul的携带率及其阳性菌株临床分布状况，为多重耐药革兰阴性杆菌医院感染预防控制提供依据。

Bioleaching, by means of natural populations of Thiobacillus ferrooxidans and related Thiobacilli, for example, allows recovery of up to 70% of the copper in low-grade ores.

生物萃取，例如应用天然的氧化亚铁硫杆菌菌群和相关的硫杆菌，使低品级的矿石中铜的收率达到70％。

According to pastuerella toxA gene rank, a pair of primer was designed and synthesized. The rank of toxA was amplified by pastuerella toxA in clinical.

根据巴氏杆菌毒力基因toxA序列设计合成1对引物，以临床分离的虎源巴氏杆菌为模板，通过PCR技术，扩增出toxA基因片段。

Pathogeny of northeast tiger was separated and identified in Jilin City Jiangnan Park. In order to carry out the epidemiological investigation and provide the accuracy diagnose and treatment measures, toxA gene of pastuerella was researched in the experiment.

通过对吉林市江南公园送检东北虎病例的病原分离鉴定，分离出多杀性巴氏杆菌，为进一步加强对该病的流行病学调查，并为准确诊断和治疗该病提供依据，实验对该株巴氏杆菌毒力基因toxA进行了研究。

A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果：1、用PCR方法检测A组链球菌：以A组链球菌致热性外毒素基因speB为靶序列，设计的扩增引物对全部对照菌株的扩增结果为阴性，而全部A组链球菌参考株均能扩增出特异的345bp片段，其中包括三株猩红热链球菌，检测敏感性为6.5pg／μl DNA.2、用PCR方法检测白喉杆菌：以白喉外毒素基因toxB为靶序列，设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段，而全部对照株的扩增结果为阴性，检测敏感性为850fg／μl DNA.3、用PCR方法检测嗜肺军团菌：以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因，设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段，而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

The results showed as follows: the optimum concentration of Tribulus Terrestri for the culture of L.

研究了不同浓度的蒺藜药液及药液离心与否对嗜酸乳杆菌和保加利亚乳杆菌生长的影响。

According to the nutritional needs of B.bifidum,based culture and stimulated factors were optimized by quadratic design.The optimum based culture was as following: yeast extract was 0.9%,tryptone was 0.3%,soya peptone was 0.3%,glucose was 2.0%,bifidus was 0.8%,growth factor was 10%.

针对两歧双歧杆菌的营养需要，采用正交试验设计优化增殖培养基，结果表明，酵母膏是影响短双歧杆菌的显著因素，最佳的优化培养基配比是：酵母膏0.9%，胰蛋白胨0.3%，大豆蛋白胨0.3%，葡萄糖2.0%，双歧因子0.8%，生长因子10%。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。