杆菌

The ferment product of strains JF and Al with a broad spectrum of antimicrobial activity, against one or more sensitive microbes, such as Staphylococcus epidermidis, Enterobacter aerogenes, Escherichia coli and Typhoid bacillus etc, were screened out using the filter paper assay, strain C6 have inhibiting effects on VSMC proliferation.

结果：从槲寄生中分离到JF、A1、G6等11株内生真菌；G6对牛主动脉平滑肌细胞增殖有抑制作用；JF、A1发酵产物具有较广语的抗菌活性，对革兰氏阳性细菌如表皮葡萄球菌，革兰氏阴性细菌如产气肠杆菌、大肠杆菌、伤寒杆菌等均具有较强的抑制作用。

The aim of this research is to find out the experimental parameters that affecting the results when analyzing bacteria by MALDI TOF MS, and to set up a general sample preparation protocol for establishing mass database of Bacillus anthracis and Yersinia pestis. It will play an important role in rapid identification of bacteria, and tracing its origin in the scenario of bioterrorism and outbreak of infectious disease for microbial forensic analysis. Methods:As for the universalness of sample preparation protocol for bacterial analysis by MALDI TOF MS, bacteria being investigated included: gram-positive bacteria including B.

本研究通过对MALDI TOF MS分析细菌的方法进行系统的研究，阐明影响MALDI分析的重要实验因素的作用，并建立MALDI TOF MS分析细菌的标准实验方法，从而实现不同菌种和同一种内不同菌株的鉴定，为建立炭疽芽孢杆菌和鼠疫耶尔森氏菌的质量图谱数据库奠定基础，从而能够快速准确地检测和鉴定我国不同来源的炭疽芽孢杆菌和鼠疫耶尔森氏菌，在生物恐怖或传染性疾病爆发时，作为微生物法医学的一种重要技术，快速、准确地鉴定细菌，并追踪其来源。

Except for verdigris pseudomonas,Gram-negative bacilli were less resistant to levofloxacin than to the other three ones.Klebsiellas,verdigris pseu-domonas and acinetobacters were30.76%～33.33%resistant to ciprofloxacin.

除铜绿假单胞菌外革兰阴性杆菌对左氧氟沙星的耐药率均低于其他3种药物，克雷伯菌、铜绿假单胞菌、不动杆菌对环丙沙星耐药率在30.76%～33.33%。

Compared with 11 other isolates reported in GenBank, there was slightly variation in the nucleotide sequence of Omp25 gene of wapiti's isolate. The identities of nucleotide sequence and deduced amino acid sequences among 11 strains were 99.1%-99.4% could 99.2%-99.5% respectively.

与GenBank中已经登录的布氏杆菌其他11个分离株相比，马鹿布氏杆菌分离株Omp25基因变异较小，以散在的点突变为主，Omp25基因核苷酸和推导氨基酸序列的同源性分别为99.1%-99.4%和99.2%-99.5%之间。

The recorded genera are as follows: Pseudomonas, Flavobacterium, Alcaligens, Achromobacler, Zoogloea, Bacillus, Micrococcus and Arthrobacter.

经鉴定，所获菌株归纳为八个属，其中数量最多的优势属是假单胞杆菌属和黄杆菌属。

The sludge in SBMBR1 presented granular characteristics and settled very well. The sludge in SBMBR1was brown and the sludge flocs was smashed by the shear force due to high intensity of aeration. The sludge size decreased and the sludge flocs blended with water forming ropy solutions, and there was no distinguish interface between water and sludge. Low sludge age and high loading operation enabled the sludge in SBMBR1 keeping higher sludge activity and the MLVSS/MLSS ratio and SOUR values of SBMBR1 were higher than SBMBR2. Both in SBMBR1 and SBMBR2, there were large amount of zoogloea, bacillus, coryneform of bacteria, and pediococcus. However, in SBMBR1 there were also a lot of Protozoa like epistylis and etc. A certain amount of filamentous bacteria can also be found in SBMBR1. The biological species in SBMBR1 were much more abundant than that in SBMBR2.3. Membrane fouling on flat-sheets membrane was observed and analyzed with AFM, SEM and TEM. After the measurement of membrane surface EPS and membrane surface ETX analysis, the pollutants composition on the surface of flat-sheets membrane was calculated.

从外观观察SBMBR1反应器中的活性污泥呈浅黄色，随着运行时间的增加，污泥粒径逐步增加，SV、SVI值逐步下降，呈现出较强的颗粒特征，沉降性能良好；SBMBR2污泥呈土黄色，污泥絮体被高强度的曝气所产生的切应力所打碎，污泥粒径迅速降低，絮体较为细碎，和水相混合成均一粘稠的溶液，看不到泥水分界面；低泥龄、高负荷运行，使得SBMBR1可以保持较高的微生物活性，MLVSS/MLSS值、SOUR值均高于SBMBR2；两级反应器活性污泥中均有大量的菌胶团，长杆菌、短杆菌和球菌是构成主体，但SBMBR1活性污泥中含有大量固着型原生动物如累枝虫等，还存在着一定数量的丝状菌，生物相要比SBMBR2丰富。3、借助原子力显微镜、扫描电镜、透射电镜等手段对平板膜污染现象进行观测和分析，并通过膜表面污染物EPS测定、污染膜表面能谱分析，得出平板膜表面污染物的组成。

The production of Feeding Bacillus Microbiological- Candy Peptide Element is a kind of additive with high-quality , high-efficient , and without public nuisance. It is consist of some active substance as feeding bacillus and so on, and it is refined by the technology of contemporary cell, gene and the regulation of zymolysis.

项目简介：饲用芽孢杆菌微生态复合制剂-"糖肽素"产品是一种优质高效无公害添加剂预混合饲料，是由饲用芽胞杆菌等活性物质组成的微生态复合制剂，经现代细胞和基因工程及代谢调控发酵技术精制而成。

32 U/mL enzyme activity can be detected in medium after fermentation. By optimizing the zymotic medium and fermentation conditions, recombination B. subtilis can express as high as 110.02 U/mL of Alkalineβ-mannanase. Cycle of fermentation needs 5 days(4 days is needed in induced expression ), then enzyme activity maintained at the highest level, less than 5 % enzyme activity was lost in next 2 days.

为了继续对碱性β-甘露聚糖酶进行高表达研究，试验采用了芽孢杆菌发酵培养基来进行产酶研究，初步诱导酶活达到29.32 U/mL，通过对发酵条件的优化，该重组枯草芽孢杆菌的产酶水平有了大幅度的提高，最高可达110.02 U/mL。

Abjective to express the Hepatitis A virus genome cDNA attenuated salmonella Typhimurium and to find the immunity to hepatitis A virus after administrated that salmonella Typhimurium with hepatitis A virus gemome to the mousehepatitis A virus coding region cDNA were inserted expressive plasmid PBV221 and then were transform into E coli DH5α.

使甲型肝炎病毒基因组cDNA能够在减毒伤寒杆菌中表达，从而通过口服此种减毒伤寒杆菌达到对甲肝免疫的作用。将甲肝病毒编码区cDNA插入高效表达质粒PBV221，转化大肠杆菌DH5α，筛选并鉴定阳性克隆。

Of them were Gram-negative rod, 3.8% were Gram-positive. The dominant genera were Vibrio(28 strains). The genera were also composed of Flavobocterium(6), Pseudomonas(5), Achromobacter(5), Aeromonas(3), Enterobacteriaceae(2), Bacillus(2). 1 strain isolated from the oyster died in reproduction.

优势菌为弧菌属28株，其次是黄杆菌属6株、假单胞菌属5株、无色菌属5株、气单胞菌属3株，此外还有肠杆菌科2株、杆状菌属2株，另有1株细菌在传代中失去了生长能力。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。