杆菌

The genus Mycobacterium consists of nonmotile,nonspore - forming aerobic bacilli,(0.2~0.6) m m ×(1~10) m m in size.

分枝杆菌属是一类细长或稍弯的杆菌，因有分枝生长的趋势而得名。

Although nonviable bacilli and metabolite did not improve apparent digestibility of energy in crop, they improved the apparent digestibility of energy in ileum.

高剂量（10〓cfu／g）死菌制剂显著降低嗉囔中乳酸杆菌数量（P.05），低剂量（10〓cfu／g）死菌制剂和口服代谢产物不显著影响嗉囔中乳酸杆菌和大肠杆菌数量。

We also compare the correlation between normal PCR bifidobacteria quantification and traditional diluting bifidobacteria quantification.

并且比较普通PCR定量的双歧杆菌结果和传统稀释滴种法定量的双歧杆菌结果的相关性。

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

Go in all bacteriological examine 94 second, achieve positive result 79 second, rate of positive sending check is amounted to 84.04%, unplug before the canal every week 1 in all 64 bacteriological in examining, electronegative 8 second, occupy 12.5%, pure bacterium affects 52 second, 2 serious infection 1 second, bacterium, fungus mixes infection 3 second, the pathogenic bacteria inside spirit way is phyletic with change bacili of negative of orchid family name is given priority to, bacterium of verdigris holiday single born of the same parents is the first place.

结果首次细菌学检验有23例分离出致病菌，阳性率65.7%，其中革兰氏阴性杆菌17例，占73.9%。35例共行细菌学检验94例次，获得阳性结果79例次，送检阳性率达84.04%，拔管前每周1次共64次细菌学检验中，阴性8例次，占12.5%，单纯细菌感染52例次，二重感染1例次，细菌、真菌混合感染3例次，气道内致病菌种类以革兰氏阴性杆菌为主，铜绿假单胞菌为首位。

This finding does not support a significant role for Mycobacterium avium subspecies paratuberculosis in the pathogenesis of Crohn's disease in the majority of patients.

这个发现也不支持鸟分支杆菌的亚型副结核分支杆菌是大多数克隆氏病患者的病因。

This finding does not support a significant role for Mycobacterium aium subspecies paratuberculosis in the pathogenesis of Crohn's disease in the majority of patients.

这个发现也不支持鸟分支杆菌的亚型副结核分支杆菌是大多数克隆氏病患者的病因。

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能，本研究选择了MAP的主要保护性抗原Hsp65蛋白，以副结核分枝杆菌C-2株染色体DNA为模板，以hsp65基因的特异性引物进行PCR扩增，获得了1 626bp的hsp65基因，通过T-A克隆技术，将PCR产物克隆至pGEM-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pGEM-T-hsp65，以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列，结果表明，所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致，两者核苷酸序列的同源性为99.1％，氨基酸序列的同源性为99.3％，表明该基因在副结核分枝杆菌中高度保守。

Moreover. These results would be helpful for future research on pathogenicity of Shigella spp.

这些结果加深了我们对痢疾杆菌中H-NS蛋白的认识，有助于痢疾杆菌致病机理的研究。

The main obstacles to the global control of the disease are emerging multi-drug resistant strains of Mycobacterium tuberculosis and the recalcitrance of persistent infections to treatment with conventional anti-TB drugs.

目前，结核分枝杆菌的多重耐药，以及在抗结核药物作用过程中结核分枝杆菌的持留状态，已成为全世界结核病控制工作的主要障碍。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。