杆菌

In order to investigate new methods to the detection of Mycobacterium tuberculosis and diagnostic value of FQ-PCR in the detection of DNA in Mycobacterium tuberculosis, FQ-PCR was used to detect DNA of Mycobacterium tuberculosis in body fluid from 68 patients with tuberculosis and 68 patients without tuberculosis and it was also compared with the two methods of smear acid-fast stainning and antituberculotic antibody.

为探讨结核分枝杆菌检测的新途径及其FQ-PCR技术在结核分枝杆菌DNA诊断中的实用价值，运用FQ-PCR技术对68例结核病患者和68例非结核病患者体液进行结核分枝杆菌DNA测定，并与涂片抗酸染色及ELISA—抗结核抗体法进行比较。

Result: In blood culture, Pseudomonas aeruginosa accounted for 23.5％ remained in the first place, while Staphylococcus epidermidis fell to the third place. In group 2, Gram negative bacillus accounted for 58.8％ and Gram positive coccus 29.4％. In wound culture, Pseudomonas aeruginosa ranked the top (19.5％) while Staphylococcus aureous fell to the sixth, Gram negative bacillus accounted for 73.5％, Gram positive coccus 22.2％.

结果：两组比较，血培养显示铜绿假单孢菌继续保持第一位，占23.5％，表皮葡萄球菌退居第三位，其中第二组G－杆菌占58.8％，G＋球菌占29.4％；创面培养，铜绿假单孢菌上升至第一位，占19.5％，金黄色葡萄球菌退至第6位，其中第二组G－杆菌占73.5％，G＋球菌占22.2％。5年内重要菌群G－杆菌居首位，近3年常用抗菌药物如头孢他啶等耐药性较前2年明显增加。

The 19 strains of bacilliform bacteria were identified by biochemical tests, part of their 16S ribosomal DNA were amplified, sequenced, and phylogenetically placed.

分离得到得19 株乳杆菌通过菌落、菌株形态观察以及多项生理生化等常规鉴定，并且通过16S rRNA 基因序列测序鉴定，确定这19 株乳杆菌分属于乳杆菌属内专性同型发酵类型中三个不同的种。

In order to analyze the specificity of multiple PCR, multiple PCR detection system was used to detect Blastomyces albicans, Chlamydiae trachomatis, Escherichia coli, hemophilies influenzae, Klebsiella pneumoniae, Staphylococcus aureus, Streptococcus pneumoniae, Human cytomegalovirus, duovirus, adenovirus and Henrietta Lacks strain of cancer cells.

用此多重PCR体系分别检测分离的白色念珠菌、沙眼衣原体、大肠埃希氏杆菌、流感嗜血杆菌、肺炎克雷伯杆菌、金黄色葡萄球菌、肺炎链球菌、人巨细胞病毒、轮状病毒、腺病毒和Hela细胞，分析此多重PCR体系的特异性。

When the mixture of bacteria strain MH and fungi strain Cladosporium is used, the extent of diesel biodegradation is significantly increased by 80% after five days; Diesel pollutants in aqueous solution can be completely removed by synergistic use of these MH and Cladosporium; The observed synergistic effect is closely related to the aromatics-resistance of the fungi strain Cladosporium, which in turn favors the growth of the MH.

当分支杆菌菌株和枝孢属菌株混合使用时，柴油在五天后的生物降解率高达80%；通过分支杆菌菌株和枝孢属菌株的协同降解作用可完全除去水体中的柴油污染物；协同作用主要涉及到枝孢属菌株对芳香烃的降解而促进了分支杆菌菌株的生长。

Results Totally 494 strains of bacteria were isolated, 307 strains of Gram negative bacilli and 187 strains of Gram positive cocci account for 62 1% and 37 9% r...

革兰氏阴性杆菌中铜绿假单胞菌 12 2株，肺炎克雷伯氏菌和大肠埃希氏菌 5 2株，鲍氏不动杆菌 4 4株，嗜麦芽寡养单胞菌 2 7株，阴沟肠杆菌 2 3株。

Strains YL001 and YL002 formed a monophyletic clade with strains of X. nematophilus with sequence homology outweighing 99% and the sequence homology to genus Photorhabdus outweighing 94%. The lag, logarithmic, stationary and contabescence phase of the two bacteria were 0-6, 6-18, 18-66 and 66 h respectively; pH of strains YL001 and YL002 reduced to 5.70 and 5.56 at 12 h, respectively, and then rose gradually to 7.74 and 8.07 respectively when culture finished. Glucose content reduced quickly during 0-18 h, and then kept stable. Amido nitrogen content reached the lowest at 12 h and then rose slowly. YL001 exhibited the highest inhibitory effects at 54 h and YL002 exhibited highest inhibitory effects at 42 and 66 h respectively on B. cirerea and B. subtilis. Strains YL001 and YL002 belonged to X.

YL001和YL002菌株与嗜线虫致病杆菌种内菌株形成一个类群，序列同源性大于99%，与发光杆菌属内菌株的序列同源性大于94%。2菌株的延缓期、对数生长期、稳定期和衰亡期分别为0~6，6~18，18~66和66 h；培养12 h后，YL001和YL002菌株发酵液的pH值分别降低至5.70和5.56，此后逐渐上升，至发酵结束时其pH值分别为7.74和8.07；培养0~18 h时2菌株发酵液中还原糖含量迅速降低，此后保持稳定；12 h时氨基氮含量达到最低，此后开始缓慢上升；YL001菌株培养54 h后及YL002菌株培养42和66 h后，其对番茄灰霉病菌和枯草芽孢杆菌的抑制作用最强。

The main results are illustrated as following: 1. With the probe of the argB gene from Corynebacterium glutacium ATCC13032, it was made sure that the argB genes between C. crenatum A. S 1. 542 and C.

主要研究结果如下： 1 通过Southern Blotting实验，以谷氨酸棒杆菌ATCC 13032的argB基因为探针，确定钝齿棒杆菌A.S.1.542与谷氨酸棒杆菌ATCC 13032有较高的同源性。

Difficile is not as strong as that between the infection and antibiotics.

然而，他强调，胃酸分泌抑制剂与难治梭胞杆菌之间的关系并不像感染与抗生素之间那样紧密；必须要重视的是，感染难治梭胞杆菌的最大风险是使用抗生素，而且住院老年病患的风险最高，我们必须了解难治梭胞杆菌引起的感染越来越常见，且越来越严重，因为新的抗药性菌种已经被发现，因为感染这些高抗药性细菌的病患对治疗反应不佳，这样的课题已经受到重视。

Strains of endophytic bacteria were isolated from four cotton varieties after a strict surface-sterilization among which, 102 strains were subordinate to Pseudomonas, Erwinia, Xanthomoas and Bacillus genera.

从4个不同棉花品种体内分离到内生菌503株，鉴定了102株，分别属于假单胞杆菌属、黄单胞杆菌属、芽胞杆菌属和欧文氏菌属。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。