杆状

Bovine Ephemeral Fever is a bovine viral disease that is transmitted by culicoides, its outbreak in Taiwan and Southeast Asian countries is periodical.

牛流行热(bovine ephemeral fever； BEF)是由杆状病毒经库蠓媒介传染的牛病毒性疾病，本病在台湾及亚洲国家中呈周期性流行。

Objective To express and locate a fusion gene consisting of c-Myb DNA-binding domain and Myeloid elflike factor AML1-binding region by using baculovirus vector.

目的 将C-Myb的DNA结合域与MEF的AML1结合区组合成的MM融合基因在杆状病毒中进行表达和细胞定位。

The mice inoculated with Coxiella burnetii intrana- sally developed interstitial pneumonia,while the primary pathological changes of mice inoculated intraperitoneally are granulomas in spleen and liver.2.The pathological changes became more severe followed the dosage increasing.3.Coxiella burnetii can be detected in spleen and liver at day 2 after inoculation.the lesion became more and more serious from day 2 to day 12.The characteristic changes were observed at day 7,and recovered at day 14. 4.The reticuloendothelial system are main target of Coxiella burnetii.The pathogen was detected in cytoplasm of monocyte -macrophages of spleen, liver, lung, and endothelioid cells of blood vessel. 5. Coxiella burnetii can be found in macrophages lysosomes by electron microscopy. Most of them are round or rod, and polymorphic shape can also be observed in different size.

结果：1、通过不同感染途径的实验证实，滴鼻感染的小鼠主要表现为间质性肺炎，而腹腔注射感染小鼠则以脾脏、肝脏肉芽肿为主要病变。2、通过不同剂量的感染实验发现，随着感染Q热立克次体剂量的加大，动物病变愈加严重。3、通过感染后不同时间的动态病理学观察发现，在腹腔注射后第2d的脾和肝脏即可发现病原体，主要脏器的病理变化从第2d到第12d逐渐加重，第7d动物的病变最典型，至感染后14d动物的受损器官已开始出现修复性变化。4、 Q热立克次体主要侵害机体的网状内皮系统，在感染小鼠的肝、脾、肺和外周血管单核巨噬细胞以及血管内皮细胞胞浆中查见病原体。5、透射电镜观察可见Q热立克次体主要位于巨噬细胞吞噬溶酶体内，呈多形性，多见圆形和杆状，大小不一。

Any of various gram-negative, rod-shaped, mostly aerobic flagellated bacteria of the ''.

假单胞''。'菌'。''任一种革兰氏阳性杆状的假单''。

Legionellae are aerobic, non-spore forming, rod- shaped , typically flagellated, gram-negative bacteria.

军团菌群是一种好氧的、非孢子繁殖的、杆状的、一般带有绒毛的格兰氏阴性菌。

Any of various gram-negative, rod-shaped, mostly aerobic flagellated bacteria of the phylum Pseudomonad, commonly found in soil, water, and decaying matter and including some plant and animal pathogens.

假单胞菌任一种革兰氏阳性杆状的假单菌门雷氧鞭毛虫细菌，一般见于土壤、水、腐败物，还有一些植物和动物的病原体中

An aerobic culture utilizing 4-CNB as the sole source of carbon, nitrogen, and energy, named as A1 has been isolated from the acclimated aerobic sludge, it was Grame positive, the optimal condition for 4-CNB degradation were pH 7.5, the maximum specific substrate degradation rate of 4-CNB was 0.505mg.

从驯化的好氧污泥中分离得到小＊＊B好氧降解菌川，杆状，G＼降解试验发现最适叫7.5，最佳接种量580m扒100mL；该菌株降解4七NB的最大比降解速率为0.505＊g。

In order to improve the viral detection method in shrimp, a more rapid and convenient multiplex reverse transcription-polymerase chain reaction was established in the present study. The new method was used for detection of shrimp viruses, including Yellow-head virus, white spot syndrome virus, Taura syndrome virus, hepatopancreatic parvovirus, infectious hypodermal and hematopoietic necrosis virus and monodon baculovirus.

为了提高对虾病毒检测的快速性和简便性，建立了一种可同时检测6种对虾病毒的RT-PCR方法，并利用该方法对来自广西沿海地区的130份病虾样品中的黄头病毒、白斑综合征病毒、桃拉病毒、肝胰腺坏死病毒、传染性皮下组织坏死病毒以及杆状病毒进行了检测。

PCR ; Hypodermal and hematopoietic necrosis baculovirus ; Penaeid shrimp ; Explosive epidemic disease

PCR法；皮下和造血器官坏死杆状病毒；对虾；暴发性流行病

In order to control the explosive epidemic disease of Penaeid shrimp in China, the one tube multiplex polymerase chain reaction were developed for detection of hypodermal and hematopoietic necrosis baculovirus.

根据对虾皮下和造血器官坏死杆状病毒运动HHNBV－XIA靶基因序列设计了4个多重PCR法用引物（P1、P2、P3、P4）。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。