杆状

Any of various gram-negative, rod-shaped, often pathogenic bacteria of the genus Streptobacillus, occurring in chains, especially S.

链杆菌一种革兰氏阴性的杆状，经常为单性生殖细菌链杆菌属，呈链形，尤指引起一种''。

The morphological characteristic of streptococci is their tendency to grow in chains .

链球菌的形态学特征是：外形似卵圆形或短杆状，呈链状生长，在分裂之前生长时平行延伸成链。

We established baculovirus expression vectors of 49ku ES structural protein gene and obtained recombinant protein.

本实验将本地毛形线虫ES抗原的49ku结构基因构建到杆状病毒表达载体上，并在昆虫细胞中得到表达。

Objective In order to make a foundation for molecular diagnosis and gene engineering studies of Avian encephalomyelitis virus, the structural protein VP3 gene of AEV and expression of its recombinant protein were cloned and sequenced.

目的对VP3进行克隆和测序后，在杆状病毒系统中表达获得重组蛋白，为AEV的分子诊断以及更深一层次的分子和基因工程研究打下基础。

Methods The HPV16E7 gene was cut to three parts, amplified by PCR, and connected individually with BPVL1 on plasmid PUC. With PVL1393 as a transfect vector, the BPVL1/HPV16E7 recombinants were transfected to baculovirus which subsequently expressed the chimeric BPVL1/HPV16E7 protein in the SF－9 cells. The recombinant proteins were then purified by centrifuge, sonication, sucrose and CsCl ultracentrifuge, and were identified by SDS-PAGE, Western blot, ECL and TEM.

HPV16E7基因分3段经PCR扩增后分别克隆入连有BPVL1的质粒PUC形成BPVL1-HPV16E7；以质粒PVL1393为载体将BPVL1-HPV16E7基因转染杆状病毒并在昆虫细胞中进行表达；用超声粉碎和蔗糖超离、氯化铯梯度离心等方法纯化以及免疫印迹、ECL、透射电镜等方法鉴定表达产物。

Morphological and physiological identification showed that NTG-01 was Gram-negative, short rod shape, with flagella around the bacteria, V-P test positive and Methyl red test negative. The bacterium can use glucose as carbon suource and produce gases. No H2S produced on TTS medium. Lysine decarboxylase and orthinine decarboxylase, catalase and gelatine test are positive. Arginine decarboxylase, dehydrolase, oxidase and urase test are negative. Sequencing and alignment of 16S rDNA showed that it shared a 99% homologous sequence with Enterobacter aerogenes.

结果显示菌株NTG-01为细菌，革兰氏染色阴性，短杆状，鞭毛周生，V-P试验阳性，甲基红实验阴性，利用葡萄糖产酸产气，不能在TIS培养基上产生H_2S，赖氨酸和鸟氨酸脱羧酶试验呈阳性，精氨酸脱羧酶和双水解酶试验呈阴性，氧化酶和脲酶试验阴性，接触酶和明胶液化试验阳性，但后者反应迟缓，一周后才观测到结果。

Veneupis variegate ; Rod-shaped virus ; BMNV ; Spheroid Viruses

杂色花蛤；杆状病毒； BMNV ；球状病毒

Baculovirus is a kind of double-stranded circle large DNA virus, which has been developed as an environment sound pesticide and a powerful vector of foreign protein expression, as well as a potential vector of gene therapy.

杆状病毒是一类大型环状双链DNA病毒，作为一种高效的生物杀虫剂、真核表达系统以及基因治疗载体的研究和应用，其分子生物学和侵染机制的研究也取得了很大进展。

Visual purple pigment found in the outer segments of rod cells.

视觉的紫色色素，存在於杆状细胞外部。

Of them were Gram-negative rod, 3.8% were Gram-positive. The dominant genera were Vibrio(28 strains). The genera were also composed of Flavobocterium(6), Pseudomonas(5), Achromobacter(5), Aeromonas(3), Enterobacteriaceae(2), Bacillus(2). 1 strain isolated from the oyster died in reproduction.

优势菌为弧菌属28株，其次是黄杆菌属6株、假单胞菌属5株、无色菌属5株、气单胞菌属3株，此外还有肠杆菌科2株、杆状菌属2株，另有1株细菌在传代中失去了生长能力。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。