杂种的

To study the molecular mechanism of Larix somatic embryogenesis, a differentially expressed cDNA library of Larix somatic embryo in the period of maturation was constructed using suppression subtractive hybridization. The cDNA from the cultures at the stage of somatic embryo maturation of embryogenic cell line Y35 of L. leptolepis L. princi-pis-rupprechtii was used as the tester and the cDNA from its subcultured callus was used as the driver. Eight hundreds ran-domly selected positive clones were sequenced, and 468 UniGenes were obtained finally.

为研究落叶松体细胞胚胎发生的分子机理，文章以日本落叶松华北落叶松杂种无性系胚性细胞系Y35体细胞胚成熟阶段培养物的cDNA为实验组，继代培养阶段胚性愈伤组织的cDNA为对照组，利用抑制性消减杂交技术(Suppression subtractive hybridization， SSH)构建了体细胞胚成熟阶段的差异表达基因文库。

To study the molecular mechanism of Larix somatic embryogenesis, a differentially expressed cDNA library of Larix somatic embryo in the period of maturation was constructed using suppression subtractive hybridization. The cDNA from the cultures at the stage of somatic embryo maturation of embryogenic cell line Y35 of L. leptolepis × L . princi-pis-rupprechtii was used as the tester and the cDNA from its subcultured callus was used as the driver. Eight hundreds ran-domly selected positive clones were sequenced, and 468 UniGenes were obtained finally.

为研究落叶松体细胞胚胎发生的分子机理，文章以日本落叶松×华北落叶松杂种无性系胚性细胞系Y35体细胞胚成熟阶段培养物的cDNA为实验组，继代培养阶段胚性愈伤组织的cDNA为对照组，利用抑制性消减杂交技术(Suppression subtractive hybridization， SSH)构建了体细胞胚成熟阶段的差异表达基因文库。

Somaclonal variations analysis on mixoploid hybrid plant BI-1 showed that percentage of cells with 2n=25, 26 decreased and percentage of cells with 2n=29 ascended.Chromosome segments of I.

并对混倍体杂种BI-1的无性系变异进行了分析，发现染色体数目为25、26的细胞比例呈明显下降趋势，而染色体数为29的细胞比例明显上升，其他染色体数目的细胞比例处于动态变化之中。

In addition,the proportion of Typical Abortive pollen and Normal pollen acted as the fertility indexs and typical sterile group or normal fertile group was classified in segregative population according to the pollen fertility of parent or F1 hybrid.

在育性指标的划分上，本研究确定以亲本不育系及与恢复系配制的杂种F_1的花粉育性作为划分典型不育株和正常结实株的标准。

In this present study, sterile hybrid progenies derived from optimal combinations guaranteed the protection of intellectual property. Meanwhile, inter and intra-specific hybridization using male sterility and molecular method for hybrid seed identification paved a new way for crossbreeding Tagetes erecta through male sterility.

本研究所选育出的优良组合子代全部是不育类型的杂种，有力地保护了自主知识产权，利用雄性不育进行种内和种间杂交以及分子鉴定杂交种的方法，开创了万寿菊利用雄性不育进行杂交育种的新途径。

Thus it is obvious that the weak homology existedamong genomes A, B, D and 〓. However, the rare unreduced gemetes and near-unreduced ones with the loss of several chromosomes could be produced by the highlyunequtional division of univalents at the first meisis and the normal second division,which gave the F1 hybrid the partial fertility.

但通过第一次减数分裂时单价体的极不对称的分离及正常的第二次分裂，可形成少量未减数配子和丢失了少数几条染色体的近未减数配子，使杂种〓表现部分可育性。

In order to study transmission of 6VS/6AL chromosome through gametes and its genetic stability in different genetic background, the 6VS/6AL translocation line 92R137 (highly resistant to powdery mildew) were respectively crossed to common wheat varieties Bainong 64, Bainong 9310, Han 5310, Xiaoyan 54, Huaimai 20 and Xumai 856 introduced from different areas of China and the hybrids F1 were backcrossed as male and female.

为研究小麦－簇毛麦6VS/6AL易位染色体在不同小麦背景中的遗传稳定性及其在配子中的传递，利用高抗白粉病的普通小麦－簇毛麦6VS/6AL易位系92R137与不抗白粉病的栽培品种百农64、百农9310、邯5310、小偃54、淮麦20、徐麦856进行杂交，并用这些品种分别与杂种Fl进行正反回交。

Just to make sure nothing would go wrong, several of Bruno's assistants stood at a discreet distance, each with a pit bull terrier on leash.

为了保证不发生意外，布鲁诺的几个助手站在不显眼的远处，每个人手里都牵着一个拴着狗皮带的好斗的杂种狗。

According to cytology, biology, oil chemical characteristic and castor-oil plant's physical and chemical properties, it surveys both domestic and international research on the progress and development of castor-oil plant hereditary improvement, hybrid vigour utilization and comprehensive utilization.

研究立足于蓖麻遗传多样性及其植物质能的综合利用潜势，根据蓖麻雌雄同株的细胞学、生物学和油脂化学特性和毒蛋白的物化性质，综述国内外有关蓖麻生物学、遗传育种、杂种优势及综合利用的进展与研发前景。

All cultivars of citrus are susceptible to canker and highly susceptible when being artificially inoculated,implying that no function genes resistant to canker resistance have been identified in citrus,pthA is essential for the pathogen to cause hyperplastic canker symptoms on citrus.

然而，到目前为止，尚无一种能根除柑橘溃疡病菌的最佳方法，柑橘品种资源中亦未发现完全抗病的品种，长期的常规育种，也没有获得抗柑橘溃疡病的优良杂种，柑橘抗溃疡病育种没有取得实质性进展。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。