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"You dirty bastard," his friend said, and tried to kick me in the g roin.

&你这个杂种&,他的朋友一边骂一边用脚踢我的下身。

Seven restorers were selected out of 15 resource materials, based on their complete restoring ability to both of the ms lines.

利用这7个恢复系与湘油66A所配的组合在品种比较试验中表现出不同程度的杂种优势,6个组合均比对照增产,增产幅度为2.28%~19.21%,其中有3个组合的增产幅度在10%以上。

Methods: Blood supply of composite mandibular grafts with different blood supply pedicles was evaluated in canines and rabbits by using angiography and a three-phase technique of radionuclide skeletal imaging.

在成年杂种犬和家兔利用血管造影术和放射性同位素骨三相显像技术进行观察和测定并比较不同血管蒂下颌区复合组织瓣的血供。

Americans are chillingly scorned as miscegenated "bastards", in contrast to pure-blooded Koreans.

朝鲜人为自己纯粹的血统而骄傲,嘲弄美国人的种族混杂,令人震惊地将他们称为&杂种&。

Male sterility of wheat inducing by Chemical hybridization agents is one of the main ways to produce hybrid wheat seed.

应用化学杂交剂诱导雄性不育是小麦杂种优势利用的重要途径之一。

Three wheat spindle streak mosaic viruses resistant cultivars ('Yining Xiaomai', 'Xu87-633', and 'Xifeng') and one susceptible cultivar ('Zhen9523') were used as parents of 3 crosses in this experiment. WSSMV resistance of the parents, F1, and F2 was evaluated under field condition.

选用3个抗梭条花叶病的小麦品种&仪宁小麦&、&徐87-633&和&西风&为抗病亲本、以感病品种&镇9523&为感病亲本配制了三个杂交组合,对四个亲本及其杂种后代(F1及F2代)单株的田间抗病鉴定表明,三个抗病亲本的抗性均由核基因控制,为显性遗传方式。

After purification and quantification,1 ugof total RNAs were reverse transcribed and amplified by PCR with5'upstream 10 base random primers and one-base or two-base anchored 3'primer respectively,and the amplified products were displayed on asequencing gel side by side.

本文以父本黄早4,母本U8112和杂种一代掖单4号的玉米幼苗为材料,提取总RNA,用5'端随机引物和3'端单锚定或双锚定引物,进行了mRNA的差异展示研究。

The seed set frequencies ranged from 0.001% to 0.184%.The chromosome number of F2 plant root tip varied from 39 to 56.4 In the Fa progeny of wheat *AR7l, Some plants showed higher alien translocation frenquencies.

在小麦与黑麦AR71属间杂种的F_2中,个别植株发生了较高的异源易位频率,其中有1株(229-3植株)产生了多达3个异源易位。

The result would be used in tobacco breeding.4 SRAP technology is used to study the genetic differences among different types of tobacco, including flue-cured tobacco, oriental tobacco, maryland tobacco, burley tobacco, and air/sun cured tobacco, five pairs of primer combinations which can amplify characteristic bands has been screened. Among the five combinations, two of them (SF25*SR2 and SF3*SR26) can distinguish air-cured tobacco including maryland tobacco and Burley tobacco from the other cultivated forms; SF20*SR14 can distinguish sun cured tobacco including oriental tobacco and sun-cured tobacco; SF16*SR14 can distinguish flue-cured tobacco and air-cured tobacco, and SF22*SR14 can distinguish DBJ599 with specisl aroma and the other flue-cured tobacco.5 8 varieties , which represent the different types of tobacco, are divided into 3 groups. I: G-28, II:DBJ599、MD40、By21, III: Basma、Sumsun、XNS、QCT. The cluster analysis result es consistant with experience evatuation.6 Anorthogonal design was used to optimize a ILP-PCR system with 5 factors (DNA, Mg2+, dNTP, primer and Taq polymerase) at 4 levels.The result showed the optimized ILP-PCR system for Nicotiana tabacum L. was: 2uL10×PCRbuffer, 20ng template DNA, Mg2+ 2.0mmol/L, dNTP 150umol/L, primer 0.3umol/L, Taq DNA polymerase 1 U in a total of 20uL reactionsolution.

研究结果可作为烤烟与香料烟类型间的杂种鉴定利用的实验与理论依据。4、利用SRAP标记技术,研究和分析了烤烟、晒烟、晾烟、白肋烟、香料烟等不同栽培类型间的基因型差异,初步筛选出了能够在分子水平上反映不同烟草类型差异的特征性标记带:晾烟MD40、Ky17、Burley21、TN90基因型共有的特征带有两对引物组合,分别为SF25*SR2和SF3*SR26;晒烟小牛舌、青梗、小花青和香料烟Sumsun、komotini Basma、Xanthi Basma共有的特征带引物组合为SF20*SR14;NC89、K326、G-28、大白筋和MD40、Ky17、Burley21、TN90共有的特征带引物组合为SF16*SR14,烤烟中大白筋599的特征带引物组合SF22*SR14.5、利用61对SRAP引物组合,研究了普通烟草不同类型间的亲缘关系,将普通烟草类型间8个不同供试材料划分为3类别。I:烤烟类型G-28II:白肋烟类型(MD40、By21、大白筋599III:香料烟类型(Basma、Sumsun、小牛舌、千层塔)。

However, crossing fertility between them was lower than that of mating in and in, and fertilized egg of reciprocal crossing can be incubated and produced F〓 without prezygotic isolation. Among all combinations of reciprocal crossing, crossing of wild male quail×female small-type domestic quail are proved to produce the best effect.

野生日本鸣鹑与家鹑群体之间的杂交能育性低于同种群交配水平,但可以实现正、反交,产生受精卵、孵化出杂种一代,不存在合子前生殖隔离;在所有正、反交组合中,以野雄鹑×小型雌家鹑的效果最好。

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