杂录

In short, it has all traces of random miscellanea that made Shanghai simultaneously a fascinating, wonderful, and horrifying place to be.

简而言之，你可以在书中找到上海杂录的所有细节故事，它们集当时上海的雍容华贵，喧嚣繁华与鱼龙混杂于一身。

A collection of various artistic or literary works or musical pieces ;a miscellany.

杂集，杂录各种艺术'文学或音乐作品集；杂集

Fluorescence in situ hybridization was carried out to confirm the integration of HBV DNA into male pronucleus and its replication with cell division in embryonic development.(Reverse transcriptase polymerase chain reaction,RT-PCR) and immunofluoresence assay were performed to observe the expression of the HBV gene in two-cell stage.

材料与方法：成熟雄鼠麻醉后双侧睾丸注射经脂质体DOSPER包裹的HBV质粒，手术后雄鼠与超排雌鼠合笼交配，用荧光原位杂变(Fluorescence in situ hybridization，FISH)分别检测单细胞胚和二细胞胚间期核中HBV DNA的存在与复制，用逆转录聚含酶链反应(Reverse transcriptase-polymerase chain reaction，RT-PCR)和免疫荧光方法检测HBV基因在二细胞胚胎中的表达。

The inhibitor for DNA methyltransferase, 5-azacytidine (5-azaC), can demethylate the methylated DNA and restore transcription of genes. High dose homocystine can mediat DNA methylation and suppress gene expression.

应用甲基转移酶抑制剂5-氮杂胞苷(5-azacytidine， 5-azaC)可以使甲基化的启动子去甲基化，启动基因重新转录；高浓度同型半胱氨酸(homocystine， Hcy)可以介导基因甲基化而抑制其表达。

5-Aza-cdR can elevate the transcription and translation levels of p16 gene probably by its demethylation restoring p16 expression.

5-脱氧杂氮胞苷可使p16基因在转录水平和翻译水平表达增高，其机制可能是通过去甲基化使p16基因表达恢复。

Porcine mature protein IL-18 gene was amplified by RT-PCR from porcine splenocyte total RNA extracts and then was cloned and sequenced.

采集6月龄河南良杂猪的脾脏，分离淋巴细胞后直接提取总RNA，进行反转录－聚合酶链反应扩增，扩增产物进行T-A克隆、测序，获得了河南良杂猪IL-18基因成熟蛋白序列。

The PTZ plasmid vector was changed to plasmid vector pSP72, which contains both T7 and Sp6 promotors for transcription of antisense (T7) and sense (Sp6) RNA probe in vitro. The results revealed the label rate of cRNA probe is high, the hybrids between RNA-RNA are stable, so the background of hybridization is satisfactory.

本实验将质粒载体PTZ转换为带有T7和Sp6两个启动基因的pSP72载体，故可在体外转录cRNA时同时得到意义（Sp6为启动基因）和反意义（T7为启动基因）探针，实验结果表明，cRNA探针标记率高，杂合物稳定，故所获杂交反应背景较为满意。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。