杂交植物

Key words: ornamental plants and horticulture; molecular breeding; transgenic chrysanthemum; genetic stability; cross breeding; chalcone synthase gene

关键 词：园林植物与观赏园艺；分子育种；转基因菊花；遗传稳定性；杂交育种；查尔酮合成酶基因

Nutrition solution culture, ICP-AES, the differential centrifugation technique and sequential chemical extraction method, suppression subtractive hybridization , RAPD were used in this thesis to study the mechanisms of copper tolerance and accumulation by Commelina communis grown in copper mine deposit which was reported to be a Cu hyperaccumulator, compared with C.

论文以本实验室报道的生长于矿山上的铜超富集植物鸭跖草和生长于正常土壤中的鸭跖草为试验材料，分别运用溶液培养法、ICP-AES元素测定技术、差速离心法和化学试剂逐步提取法、抑制消减杂交技术、RAPD技术研究了矿山和非矿山两种生境鸭跖草对铜的耐性和吸收积累的差异，两种生境鸭跖草在铜胁迫下养分吸收和积累的差异，两种生境鸭跖草在铜胁迫下生理反应的差异，以及铜在鸭跖草细胞内的分布及结合形态研究，矿山鸭跖草差异表达的cDNA克隆，铜对鸭跖草种群遗传分化的影响，从这几个方面探讨了矿山鸭跖草对铜的耐性机理。

Plant protoplasts from several species of plant were fused successfully by electrofusion method, and a good procedure for electrofusion of plant protoplasts in wideinterval chamber was established according to the results.

在植物原生质体的电融合研究中，着重进行了甘蓝与大白菜原生质体的杂交融合和异核体培养，并建立了实用的电融合方法和操作程序

False positive were lowered to an acceptable rate by this protocol, therefore, a big amount of work on fingerprint to get rid of false positive clones can be avoided. When whole BAC clone containing one locus of a multi-loci probe were applied for library screening, no matter homogenic BAC or heterogenic BAC, site-specificity is higher than short DNA fragment probes.

对于无法通过小基因组填补间隙的区段，可以本物种BAC克隆直接进行染色体步移，采用抑制重复顺序杂交法能够有效抑制大量存在于植物基因组中的重复顺序的干扰，把假阳性率降低到可以接受的范围，从而避免了用大量指纹分析剔除假阳性克隆。

The affinity among hybridizable species can also be speculated by the extent of homology of their repetitive DNA.

高等植物DNA的很大部分是由重复顺序所构成，本文讨论了检测亲本及子代基因组内重复顺序的异同程度作为远缘杂交的分子水平指标是简便可行的。

A full-length cDNA encoding sucrose: sucrose 1-fructosyltransferase from Lactuca sativa was inserted into pCAMBIA1300-als under the control of the CaMV 35S promoter. This plasmid was used for Agrobacteriummediated tobacco leaf disc transformation. Transgenic plants were analyzed by PCR and Southern blotting. 1-SST gene expression was confirmed by RT-PCR.

将从莴苣中克隆的1-SST基因重组到pCAMBIA1300-als中，构建了在CaMV 35S启动子调控下的植物表达载体，利用农杆菌介导的叶盘转化法将1-SST基因导入烟草中，PCR和Southern杂交检测表明获得了转基因植株，RT-PCR结果表明该基因在烟草中正常表达。

Fast-growing and tightly branched hybrid of Ligustrum ovalifolium and Ligustrum obtusifolium.

长速快的枝条紧密的由加州女贞和钝叶水蜡树杂交而成的植物。

Interspecies hybridization and backcrossing is a means to transfer desirable genes from one species to another in breeding programs of higher plants. Monosomic alien addition lines can be produced via addition of single chromosome of an alien donor species to the entire chromosome complement of the recipient species.

在高等植物中，以种间杂交和回交把有益基因从一个物种转移到另一个物种为目的育种项目中，单个外源染色体常常被附加到含有受体细胞完整一套染色体中，形成异源单体附加系。

This paper indicatesthat the Triticum-Agropyron octaploid species and the alien substitute line containAgropyron DNA sequence in different proportion. This result is consistent with thecytological and genetic analysis.

高等植物DNA 的很大部分是由重复顺序所构成，本文讨论了检测亲本及子代基因组内重复顺序的异同程度作为远缘杂交的分子水平指标是简便可行的。

This paper reports on our observation that progenies derived from rice plants pollinated by pollens of Oenothera biennis exhibited distinct variations in numerous morphological and developmental traits.

本文报道通过水稻（品种通育211）与柳叶菜科植物—-月见草进行远缘杂交，其后代出现了大量、明显的性状变异。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。