杂交

Methods Retrospective analysis of the hospital from 2005 to 2008 to cross vascular surgery in 20 cases of lower extremity arterial occlusive disease.

回顾性的分析我院2005年至2008年来血管杂交手术治疗20例下肢动脉硬化闭塞症的资料。

Morphological and Cytogenetics of octoploid trititrigias-Maicao No. 9, distant bred from the progeny of common wheat and thinopyrum intermedium were studied.

对普通小麦与中间偃麦草远缘杂交后代中选育的八倍体小偃麦－麦草9号进行了形态学和细胞学鉴定。

In the progenies of octoploid Triticale Jinsong49 crossed with octoploid Tritileymus, a stable germplasm line named Shannong030-1 was obtained.

利用细胞学、染色体C分带和原位杂交方法，对八倍体小黑麦劲松49与八倍体小滨麦杂种后代选育的稳定遗传材料山农030-1进行了鉴定。

The trigerieric hybrid involving Triticum, Leymus and Thinopyrum has been created by the cross between Octoploid Tritileymus and Octoploid Tritithinopyrum, and the morphology, fertility and cytology of the F1 hybrids was studied.

利用八倍体小滨麦和八倍体小偃麦杂交，创制出小麦－滨麦－偃麦草三属杂种，对杂种F1进行了形态学、育性和细胞学分析。

GISH analysis showed Triticale have 2 items chromosomes presented yellow and others chromosomes presented chrysoidine, because yellow chromosomes combined the Thinopyrum intermedium DNA, others chromosomes dye by PI. 4. Analysis of resistance gene: CH-9916 is the hybrid progenies of Octoploid tritielytrigia TAI-7045 X common wheat "Jinmai33".

GISH分析结果表明，小偃麦衍生系CH-9916的42条染色体中有2条外源染色体因结合有探针DNA而呈现出较强的黄色杂交信号，而没有结合上探针DNA的普通小麦染色体经过PI染色呈现出桔红色，初步推断小偃麦衍生系CH-9916为一个"小麦一中间偃麦草"的代换系。

In this study, we investigated the gene expression pattern of Cbfal in odontoblast as well as tooth development by gene cloning and in situ hybridization. The results revealed the essential role cbfal plays in tooth development.

本研究采用基因克隆与原位杂交的方法，研究了cbfal在牙乳头细胞及牙胚发育中的表达，初步证实了cbfal在牙胚发育中的作用，同时，作为整体实验设计的一部分，本研究所构建的cbfal cDNA探针及反义核酸cbfal真核表达载体为对cbfal进一步的研究奠定了基础。

II METHOD1 chose one of 3A level hospitals dentistry to carry through sanitation quality investigation of the dental diagnosis and treat instruments. Examined appliance include air turbine dental handpieces, punch-pin, odontoscope, bougie, tweezer, flex-tray that were so common used by dentists and daubed the inside or outside surface of the dental appliance and collected it; Examined targets were HBsAg, HBeAg and HBV-DNA (detected it with molecule spot cross method).2 make good use of the air turbine dental handpieces bracket jack which was all available in every china hospital dentistry and design outside shape and inside structure of the binary disinfectant-box for the air turbine dentalhandpieces.

二 方法 1 选取本市某所三甲医院口腔科使用的牙钻手机、车针、口镜、探针、镊子及弯盘6种常用诊疗器具行涂抹采样；检测指标为HBsAg、HBeAg、HBV-DNA；检测方法为HBsAg和HBeAg采用ELISA法，HBV-DNA为斑点杂交法； 2 充分考虑、利用现有牙钻手机支架插孔，设计牙钻手机二元消毒盒外形及内部构造；选定高效消毒剂戊二醛为消毒盒消毒腔内应用消毒液，其消毒效果及毒性鉴定试验参照卫生部消毒技术规范执行。

The goal of the program is to develop and promote the use of a high-yielding hybrid variety of oil palm.

这个项目的目标是发展和推动种植高产量的杂交油棕榈。

And 101 days after chamber implant, the chambers were coated by omentum or mesenterium in all the animals of both strain groups.

再将含杂交细胞的微孔膜扩散匣分别植入Balb/c和B6小鼠腹腔，观察匣内细胞生存情况。

This method can be used in PCR and RAPD amplification oncondition that the samples are in large quantities. Also can be used for detection ofbenzimdazol fungicide resistance in F. graminearum on DNA level.

用此方法所获得的产物可进一步用于PCR、RAPD以及Southern杂交等分子生物学研究，也为用分子生物学方法进行抗药性快速检测提供了理想的实验手段。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！