杂交

With in situ DNA hybridization, the EBV genomes have been demonstrated in the labial glands of the Sjogren′s syndrome patients (16/28), moreover the elevated content of EBV DNA has been identified in those with more severe destruction of labial glands. It is concluded that EBV, vhich has a normal site of latency at salivary glands in a nonpathogenic state, may be reactivated in Sjogren′s syndrome patients and play a role in the pathogenesis of this disease.

另外，我们用原位杂交方法观察了EB病毒DNA在干燥综合征病人唇腺组织的存在状况，结果发现28例干燥综合征病人中16例为阳性，其中腺泡组织破坏严重者，EB病毒DNA的含量亦高，并且EB病毒主要分布于大量浸润的淋巴细胞中，其次位于腺泡上皮细胞内，少量见于腺泡腔中，以上结果提示，在干燥综合征病人EB病毒处于活跃的复制状态，干燥综合征病人靶器官受损与EB病毒的活跃复制及其诱导的免疫反应密切相关。

Methods:Immunohistochemistry and nonradioactive in situ hybridization methods were used to detect the expression of p27mRNA and protein in 58 cases of osteoplastic tumors.

采用免疫组化和非放射性核酸原位杂交技术检测成骨性肿瘤中p27蛋白和mRNA的表达状况。

Sensitive and reproducible techniques of radioactive, nonradioactive and double-label in situ hybri...

原位杂交以其能原位探测基因及其表达功能，近年来得到了广泛的重视。

We detected the expression of OCY-66, OCY-189 and OCY-142 in 28 ovarian cancers and 30 normal ovarian tissues by RT-PCR, and studied the expression of OCY-142 in these ovarian cancers and normal ovarian tissues by nonradioactive hybridization in situ.

采用RT-PCR和原位杂交的方法研究卵巢癌相关肿瘤抗原新基因在卵巢癌组织和正常卵巢组织中的表达，分析其与卵巢癌临床指标的关系。

mdm2 gene expression atmrna and protein levels,p53 protein and eb virus dna were detected by nonradioactive in situ hybridization ,immunohistochemistry and polymerase chain reaction separately in 46 cases of npc tissuesand 12 cases of chronic inflammation of nasopharyngeal epithelium.

运用非同位素原位杂交、免疫组化和多聚酶链反应技术对46例npc、12例慢性鼻咽炎症粘膜分别进行mdm2 mrna、mdm2蛋白、p53蛋白及ebv-dna的检测。

Methods The effects of estrogen and progestin on the expression of NMDAR1 mRNA were investigated in rat′s cerebral cortex and hippocampus by using digoxigenin labled cDNA probe, nonradioactive in situ hybridization technique.

采用原位杂交技术，研究雌、孕激素对大鼠大脑皮层、海马N-甲基-D-天冬氨酸受体亚单位1(NMDAR1)mRNA表达的影响。

Methods We investigated the alterations of N-methyl-D-aspartate receptor 1 (NMDAR1) mRNA in rat cerebral cortex and hippocampus by using digoxigenin labled cDNA probe,nonradioactive in situ hybridization technique.

采用原位杂交技术，研究了马桑内酯致痫大鼠大脑皮层、海马N-甲基-D-天门冬氨酸受体亚单位1(NMDAR1)mRNA表达的变化。

The effect of the elastic-plastic matrix of friction soil with nonrelevant flow rule on the numerical convergence of finite element method is analyzed in this paper; and the effect of elastic modular, poisson ratio and earth pressure coefficient on numerical results are studied in this paper. At the same time, the hybrid element with a mixture of displacement and stress variables and the conventional element with solely displacement variable are compared when used in geomaterials.

在ABAQUS有限元分析软件的基础上，本文研究了服从非关联流动法则摩擦性土体的弹塑性矩阵对有限元数值算法的影响，并研究了弹性模量、泊松比以及地基土压、力系数对数值计算结果的影响，同时探讨了数值分析过程中常规位移元与杂交元在岩土工程中的应用。

The spectra, which were pretreated with the method of first derivative combined standard normal variate, were analyzed by principal component analysis.

将经一阶导数结合标准归一化处理后的光谱进行PCA降维，以前20个主成份（含原始光谱86.09%的特征信息）为输入变量，建立杂交稻种纯度鉴定的BP-ANN模型。

However, the ubiquitous expression of AmphiLysC in non-digestive tissues such as ovaries, testes, notochord, gill and muscle suggests that it may also play a non-digestive role like antibacterial defense.

但是，在文昌鱼成体其他组织如卵巢、精巢、脊索、鳃和肌肉中也检测到了AmphiLysC的表达，同时原位杂交结果也表明，AmphiLysC在文昌鱼胚胎发育的不同阶段（从2个细胞到2天幼虫）都有广泛的表达。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！