杂交

The expression of Cain in myocardium was determined by Western blot.

以蛋白印迹杂交的方法，测定心肌组织中Cain表达的变化。

Results There are 13 positive cases in 30 in situ Hybridization tissues of NPC, the p16 gene mRNA positive rate is 43.3%, and the p16 gene mRNA are positive in all of 20 cases of chronic Nasopharyngitis.

结果 30例NPC标本原位杂交阳性13例，p16 mRNA阳性表达为43.3%，3个高发家系的NPC标本无阳性表达，20例对照全部有p16 mRNA阳性表达。

Methods: The expression of EOMES gene, ETS-1 gene and MEF (myeloid Elf-1 like factor) gene in 25 cases of N-NK/T-L and 25 control cases including 11 cases of B cell lymphoma (BCL, 6 cases of T cell lymphoma, 3 cases of normal spleen, 5 cases of chronic nasopharyngitis were detected by tissue microarray and in situ hybridization.

通过对EOMES，ETS-1和MEF基因进行克隆、探针制备和组织芯片制备，对25例N-NK/T-L以及同部位11例B细胞淋巴瘤、6例T细胞淋巴瘤、3例正常脾组织和5例慢性炎性鼻黏膜组织进行了原位杂交检测。

Methods The expression level of UPA/UPAR mRNA were detected by using in stiu hybridization in the tissues of the nasopharyngnal carcinoma and the chronic nasopharyngitis.

方法用mRNA—DNA原位杂交技术检测鼻咽癌组织和炎症组织UPA和UPAR的基因表达水平。

By in situ hybridization, 53BP2 mRNA expression is revealed in tumor cell nests of NPC paraffin sections. The strong positive particles localize in the nuclei of tumor cells; but slight positive signals are also found in tissue cells of chronic nasopharyngitis and connective tissue, such as lymphocytes and smooth muscle cells; There are also positive signals in the nuclei of hyperplastic squamous epithelial cells. However, 53BP1 mRNA expression is not found in nuclei of tumor cells, tissues of chronic nasopharyngitis and connective tissue.

原位杂交结果显示，石蜡切片的鼻咽癌癌巢内，可见53BP2的强阳性表达信号，信号呈紫色颗粒状分布于胞核，核仁清晰可见；而在相同条件下，在慢性鼻咽炎组织和癌旁上皮的结缔组织，也可见弱表达信号，如淋巴细胞和平滑肌细胞；在增生的鳞状上皮细胞的核内，可找到强阳性信号。53BP1在鼻咽癌、慢性鼻咽炎组织和癌旁上皮的结缔组织切片上，均无表达信号。

This paper deals with the fertilizer rate for high—yield hybrid rice. By using the regression analysis, a hyperbola equation between soil natural nutrientsupplying rate and available nutrient content and a power functional equation between hybrid rice yield and nutrient uptake have been set up.

本文研究了高产杂交水稻的施肥量，通过回归分析建立的土壤养分自然供给量与土壤速效养分含量之间的双曲线方程和水稻产量与养分吸收量之间的幂函数方程，阐明了土壤养分自然供给量随土壤养分含量的变化而变化的规律和水稻产量与养分吸收量之间的关系。

Mab 2A3 against all haemocytes and Mab 1H11 specific to cytoplasmic granules in granular haemocytes were generated and employed to study the ontogenesis of haemocytes by IFAT in Chinese shrimp embryos of blastula, gastrula, limb bud stage, embryonic-nauplius and nauplius I stages.

1利用杂交瘤技术，论文制备了特异性识别中国对虾所有三类血细胞的单抗2A3和特异性识别中国对虾大颗粒血细胞中细胞质颗粒的单抗1H11。

Methods ①In situ hybridization was applied to observe the changes ofhypothalamic CRHmRNA positive cells after PX and melatonin supplementation;②Immunohistochemistry was used to observe the changes of CRH positive cellsin hypothalamus and CRH staining in median eminence of neurohypophysis after PXand melatonin supplementation;③ELISA was applied to detect the changes of CRHconcentration in serum after PX and melatonin supplementation.

①运用CRHmRNA 原位杂交的方法来观察松果体摘除及补充外源性褪黑素后下丘脑室旁核CRHmRNA 阳性细胞的变化；②应用免疫组织化学染色的方法观察松果体摘除及补充外源性褪黑素后下丘脑室旁核CRH 阳性细胞和神经垂体正中隆起CRH 阳性染色的变化；③运用ELISA 法检测松果体摘除及补充褪黑素后血清CRH 浓度的变化。

Methods Lymphocytes were obtained from healthy donors of different ages, both sexes, and were treated with cytochalasin B. Binucleated cells were hybridized with chromosome 21 specific probe, and simultaneously, chromosome 21 loss and nondisjunction were detected.

用细胞松弛素B处理不同年龄及性别正常人外周血淋巴细胞获得的双核细胞，以21号染色体近着丝粒特异性探针进行荧光原位杂交，同时检测21号染色体丢失及不分离。

RNAs were obtained from noninfected and Pr4- and Pr4△35-infected macrophages at 3, 8, and 24 hpi and hybridized to a radiolabeled swine IFN-α cDNA probe.

RNAs 是获得从非受传染的和 Pr4-而且 Pr4 △ 35-受传染的 macrophages 在 3 点， 8，和 24 hpi 而且对 radiolabeled 猪 IFN 使杂交-α cDNA 探针。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！