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杂交

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Recent african origin of modern humans revealed by complete sequences of hominoid mitochondrial DNAs.

同时在本实验中,20个差异显示带经Northern杂交获6个阳性克隆,占30%,这与文献报道〔8,9〕的机率相符。

Cross yellow monokaryons with white monokaryons, the fruitbody color of hybrid will show light yellows. Meanwhile, light yellows are not homochromatism, but a serial of transitional ones.

金针菇黄色单核和白色单核相互杂交,子实体颜色呈浅黄色,且浅黄色间呈现一系列的过渡色。

Gbrga2 and Gbrga8 were mapped in linkage group D02 of homology 11 at the start point, which is the same site of maker pAR286E4C and is 5.6cM away from Unig22d03bE6D marker.

本研究以海陆杂交F2群体作为定位群体,应用RFLP方法将获得的7条RGA序列定位在棉花基因组中。

Although the conservative termed secondary homothallism inherit mode and absence of wild resources, the commercial strains encompass extremely limited genetic variation, in this paper we revealed some variations of phenotypic and genorypic characteristics among the tested strains in mycelium growth rate, field performance, esterase isozyme, soluble protein and AFLP fmgerprint.

双孢蘑菇由于自身保守的次级同宗配合遗传方式以及长期野生种质资源的匮乏,造成现有栽培菌株之间相似性程度较高,尽管这样,本试验采用不同方法,从菌丝生长速度、农艺性状、蛋白质以及基因组各方面系统地反映了双孢蘑菇主要商业栽培菌株间的表型及遗传差异性,为双孢蘑菇种内杂交育种提供了科学依据。

After the horticulturist long-term hybrid cultivation, the world had more than 8000 varieties at present.

经过园艺家长期的杂交栽培,目前全世界已拥有8000多个品种。

Human reproduction needs men and women need male parent plant and plant hybrid mother, and neither is dispensable.

人类的繁殖需要男人和女人,植物的杂交需要父本和母本,缺一不可。

A method for detecting the presence of a target nucleic acid sequence in a sample, said method comprising subjecting said sample to an amplification reaction using a set of nucleotides, at least one of which is fluorescently labelled, contacting amplification product with a probe under conditions in which the probe will hybridise to said target sequence, said probe comprising a reactive molecule which is able to absorb fluorescence from or donate fluorescent energy to said fluorescent labelled nucleotide and monitoring fluorescence of said sample.

一种用于检测在某样品中是否存在目标核酸序列的方法,该方法包括:使用一套核苷酸对所说的样品进行扩增反应,其中至少一个核苷酸是被荧光标记的,在探针与目标序列杂交的条件下使扩增产物与探针混合,所说的探针包括一种活性分子,该活性分子能够从荧光标记核苷酸吸收荧光或向其贡献荧光能量,及检测样品的荧光。

Orthotopic hybridism in situ: To study the expression in situ of MMP-3 and NF-κB in frozen slices.

原位杂交:观察MMP-3和NF-κB p65在对照组和通心络组冰冻切片中的原位表达。

Preparation and collection of specimens: To kill the rabbits at different time and collect the specimens for pathology, electron microscope, RT-PCR and orthotopic hybridism in situ.

标本留取:分别于术后第2、4周处死假手术组大白兔各2只,于术后即刻随机处死对照组和通心络组大白兔各2只,损伤后3天、1周、2周、4周和6周随机处死对照组和通心络组大白兔各4只,分别留取病理、电镜、逆转录-聚合酶链式反应和原位杂交试验标本。

MATERIALS: The donor mice were the male F1 hybridism mice of cleaning inbred strain C57BL/6 and BALB/c mice or the cleaning C57BL/6 mice. The recipient was the cleaning female inbred strain BALB/c mice. These mice were used for establishing leukemia models.

材料:供鼠为清洁级近交系C57BL/6小鼠和C57BL/6小鼠与BALB/c 小鼠杂交的雄性F1小鼠,受鼠为清洁级近交系雌性BALB/c小鼠,用于构建白血病小鼠模型。

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